水稻小蛋白 OsS1Fa1 的跨膜结构域负责亚细胞定位和抗旱性。

IF 4.2 3区 生物学 Q1 PLANT SCIENCES
Plant Biology Pub Date : 2024-08-23 DOI:10.1111/plb.13711
J. S. Kwak, K. H. Lee, W. K. Min, S. J. Lee, J. T. Song, H. S. Seo
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引用次数: 0

摘要

OsS1Fa1 是菠菜 S1Fa 的同源物,是水稻中的一种小蛋白,含有四个不同的保守基团,参与抗旱。然而,这些保守基团的生物学功能迄今尚未得到表征。因此,我们研究了这些保守结构域在 OsS1Fa1 的定位和细胞功能中的作用。我们使用共焦激光扫描显微镜(CLSM)分析了粒子轰击和细菌浸润后 OsS1Fa1 的亚细胞定位。我们进行了大肠杆菌体内重组苏木酰化试验,以研究 OsS1Fa1 的苏木酰化。我们利用转基因拟南芥植物鉴定了 OsS1Fa1 跨膜结构域在抗旱中的功能。荧光分析表明,OsS1Fa1定位于核膜和细胞质膜。突变和细胞分馏分析表明,膜定位结构域决定了 OsS1Fa1 的亚细胞定位。水稻同源物 OsS1Fa2 和拟南芥直向同源物 AtS1Fa1、AtS1Fa2 和 AtS1Fa3 也表现出与 OsS1Fa1 相似的定位模式。Sumoylation分析表明,OsS1Fa1与小泛素相关修饰物(SUMO)共轭。转基因分析表明,在拟南芥中过表达 OsS1Fa1 的跨膜结构域突变形式 OsS1Fa1(TMm1)并不能提高拟南芥对干旱胁迫的耐受性,而过表达 OsS1Fa1 则能提高转基因拟南芥的耐旱性。我们的数据表明,水稻和拟南芥的S1Fa1蛋白定位于核膜和细胞质膜,跨膜结构域决定亚细胞定位,并在干旱胁迫耐受性中发挥重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The transmembrane domain of the rice small protein OsS1Fa1 is responsible for subcellular localization and drought tolerance

The transmembrane domain of the rice small protein OsS1Fa1 is responsible for subcellular localization and drought tolerance

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来源期刊
Plant Biology
Plant Biology 生物-植物科学
CiteScore
8.20
自引率
2.60%
发文量
109
审稿时长
3 months
期刊介绍: Plant Biology is an international journal of broad scope bringing together the different subdisciplines, such as physiology, molecular biology, cell biology, development, genetics, systematics, ecology, evolution, ecophysiology, plant-microbe interactions, and mycology. Plant Biology publishes original problem-oriented full-length research papers, short research papers, and review articles. Discussion of hot topics and provocative opinion articles are published under the heading Acute Views. From a multidisciplinary perspective, Plant Biology will provide a platform for publication, information and debate, encompassing all areas which fall within the scope of plant science.
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