Piotr Ladyzynski, Anna Ciechanowska, Stanislawa Sabalinska, Piotr Foltynski, Agnieszka Wencel, Cezary Wojciechowski, Krzysztof Pluta, Andrzej Chwojnowski
{"title":"血管动力学建模:层流剪切应力下葡萄糖变化对中空纤维生物反应器中 HUVEC 的影响","authors":"Piotr Ladyzynski, Anna Ciechanowska, Stanislawa Sabalinska, Piotr Foltynski, Agnieszka Wencel, Cezary Wojciechowski, Krzysztof Pluta, Andrzej Chwojnowski","doi":"10.1016/j.bbe.2024.08.004","DOIUrl":null,"url":null,"abstract":"<div><p>This study aimed to establish a blood vessel model within a hollow fiber bioreactor to evaluate the impact of high and fluctuating glucose levels on human umbilical vein endothelial cells (HUVECs) under laminar shear stress (LSS). HUVECs were cultured for 48 h in normal (5 mM), high (20 mM), and variable (20 mM / 5 mM alternating every 24 h) glucose concentrations under LSS of 0.66 Pa. An automated medium replacement system was developed. The control cultures remained static. The analysis included cell viability via cytometric analysis, glucose consumption, lactate production via electroenzymatic methods, and the expression of 21 genes via qPCR. The percentage of apoptotic cells did not significantly differ across glucose concentrations under LSS. HUVECs favor glycolysis for energy regardless of LSS. Under LSS, the <em>IL1B</em>, <em>CCL2</em>, and <em>SELE</em> genes were upregulated under high-glucose conditions and downregulated under variable-glucose conditions. A few other genes related to inflammation, oxidative stress, cell adhesion and apoptosis were upregulated under high-glucose conditions. In conclusion, using the blood vessel model we effectively examined the impact of glucose profiles on HUVECs under LSS in a device replicating the cylindrical geometry of blood vessels. LSS and tubular cell arrangement might mitigate the adverse effects of variable glucose on endothelial cells.</p></div>","PeriodicalId":55381,"journal":{"name":"Biocybernetics and Biomedical Engineering","volume":"44 3","pages":"Pages 543-559"},"PeriodicalIF":5.3000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0208521624000585/pdfft?md5=5c0c88b369931ecae917433fef387e38&pid=1-s2.0-S0208521624000585-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Modeling blood vessel dynamics: Effects of glucose variations on HUVECs in a hollow fiber bioreactor under laminar shear stress\",\"authors\":\"Piotr Ladyzynski, Anna Ciechanowska, Stanislawa Sabalinska, Piotr Foltynski, Agnieszka Wencel, Cezary Wojciechowski, Krzysztof Pluta, Andrzej Chwojnowski\",\"doi\":\"10.1016/j.bbe.2024.08.004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>This study aimed to establish a blood vessel model within a hollow fiber bioreactor to evaluate the impact of high and fluctuating glucose levels on human umbilical vein endothelial cells (HUVECs) under laminar shear stress (LSS). HUVECs were cultured for 48 h in normal (5 mM), high (20 mM), and variable (20 mM / 5 mM alternating every 24 h) glucose concentrations under LSS of 0.66 Pa. An automated medium replacement system was developed. The control cultures remained static. The analysis included cell viability via cytometric analysis, glucose consumption, lactate production via electroenzymatic methods, and the expression of 21 genes via qPCR. The percentage of apoptotic cells did not significantly differ across glucose concentrations under LSS. HUVECs favor glycolysis for energy regardless of LSS. Under LSS, the <em>IL1B</em>, <em>CCL2</em>, and <em>SELE</em> genes were upregulated under high-glucose conditions and downregulated under variable-glucose conditions. A few other genes related to inflammation, oxidative stress, cell adhesion and apoptosis were upregulated under high-glucose conditions. In conclusion, using the blood vessel model we effectively examined the impact of glucose profiles on HUVECs under LSS in a device replicating the cylindrical geometry of blood vessels. 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Modeling blood vessel dynamics: Effects of glucose variations on HUVECs in a hollow fiber bioreactor under laminar shear stress
This study aimed to establish a blood vessel model within a hollow fiber bioreactor to evaluate the impact of high and fluctuating glucose levels on human umbilical vein endothelial cells (HUVECs) under laminar shear stress (LSS). HUVECs were cultured for 48 h in normal (5 mM), high (20 mM), and variable (20 mM / 5 mM alternating every 24 h) glucose concentrations under LSS of 0.66 Pa. An automated medium replacement system was developed. The control cultures remained static. The analysis included cell viability via cytometric analysis, glucose consumption, lactate production via electroenzymatic methods, and the expression of 21 genes via qPCR. The percentage of apoptotic cells did not significantly differ across glucose concentrations under LSS. HUVECs favor glycolysis for energy regardless of LSS. Under LSS, the IL1B, CCL2, and SELE genes were upregulated under high-glucose conditions and downregulated under variable-glucose conditions. A few other genes related to inflammation, oxidative stress, cell adhesion and apoptosis were upregulated under high-glucose conditions. In conclusion, using the blood vessel model we effectively examined the impact of glucose profiles on HUVECs under LSS in a device replicating the cylindrical geometry of blood vessels. LSS and tubular cell arrangement might mitigate the adverse effects of variable glucose on endothelial cells.
期刊介绍:
Biocybernetics and Biomedical Engineering is a quarterly journal, founded in 1981, devoted to publishing the results of original, innovative and creative research investigations in the field of Biocybernetics and biomedical engineering, which bridges mathematical, physical, chemical and engineering methods and technology to analyse physiological processes in living organisms as well as to develop methods, devices and systems used in biology and medicine, mainly in medical diagnosis, monitoring systems and therapy. The Journal''s mission is to advance scientific discovery into new or improved standards of care, and promotion a wide-ranging exchange between science and its application to humans.