研究草酸艾司西酞普兰在乳腺癌共培养模型中的免疫学效应。

IF 0.4 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Asian Biomedicine Pub Date : 2024-06-28 eCollection Date: 2024-06-01 DOI:10.2478/abm-2024-0019
Nalan Biriz, Zerrin Canturk
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引用次数: 0

摘要

背景:在乳腺癌治疗期间,约有一半的患者会被处方精神药物,如选择性5-羟色胺再摄取抑制剂(SSRIs)。草酸艾司西酞普兰是一种用作抗抑郁剂的 SSRI:在这项研究中,通过用 THP-1 创造乳腺癌微环境,创建了 MCF-7 和 MDA-MB-231 乳腺癌共培养模型:方法:使用 MCF-7、MDA-MB-231 和 THP-1 细胞系来确定草酸艾司西酞普兰细胞毒性作用的浓度范围。IC50 值由 xCELLigence 实时细胞分析(RTCA)系统确定。通过流式细胞术测定细胞凋亡活性和细胞因子水平:xCELLigence 实时分析结果显示,草酸艾司西酞普兰对 MCF-7 的 IC50 值为 13.7 μM,对 MDA-MB-231 的 IC50 值为 10.9 μM。在与他莫昔芬一起进行的 xCELLigence 分析中,测得 MCF-7 和 MDA-MB-231 的 IC50 值分别为 54.6 μM 和 58.4 μM。根据 MTS 测试结果,他莫昔芬对 THP-1 的 IC50 值为 92.03 μM,对草酸艾司西酞普兰的 IC50 值为 95.32 μM。在共培养模型中,草酸艾司西酞普兰对MCF-7细胞的白细胞介素(IL)-1β、IL-6、IL-8、IL-10和TNF-α的免疫学效应分别为2.8%、11.1%、15.6%、10.6%和12.1%,而对MB-231细胞的MDA效应分别为2.1%、15.9%、16.2%、8.8%和11.8%:根据研究结果,草酸艾司西酞普兰的免疫学效应比他莫昔芬更有效,可作为乳腺癌治疗的辅助药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Investigation of the immunological effects of escitalopram oxalate in the breast cancer co-culture model.

Background: During breast cancer treatment, approximately half of the patients are prescribed psychotropic medication, such as selective serotonin reuptake inhibitors (SSRIs). Escitalopram oxalate is an SSRI used as an antidepressant.

Objectives: In this study, by creating a breast cancer microenvironment with THP-1, MCF-7 and MDA-MB-231 breast cancer co-culture models were created.

Methods: MCF-7, MDA-MB-231, and THP-1 cell lines to determine the concentration range of the cytotoxic effect of escitalopram oxalate MTS and MTT test were used. IC50 values were determined by the xCELLigence real-time cell analysis (RTCA) system. Apoptotic activities and cytokine levels were determined by flow cytometry.

Results: In the xCELLigence real-time analysis made according to the results, the IC50 value of escitalopram oxalate was measured as 13.7 μM for MCF-7 and 10.9 μM for MDA-MB-231. The IC50 value was measured as 54.6 μM for MCF-7 and 58.4 μM for MDA-MB-231 in xCELLigence analysis with tamoxifen. According to the MTS test results, the IC50 value of tamoxifen for THP-1 was 92.03 μM and the IC50 value for escitalopram oxalate was 95.32 μM. In the co-culture model, the immunological effects of escitalopram oxalate on MCF-7 cells were 2.8%, 11.1%, 15.6%, 10.6%, and 12.1% for interleukin (IL)-1β, IL-6, IL-8, IL-10, and TNF-α, respectively, while MDA effects on MB-231 cells, respectively, were 2.1%, 15.9%, 16.2%, 8.8%, and 11.8%.

Conclusions: According to the results obtained, it was concluded that the immunological effects of escitalopram oxalate are more effective than tamoxifen and that it can be used as an adjunctive agent in breast cancer treatment.

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来源期刊
Asian Biomedicine
Asian Biomedicine 医学-医学:研究与实验
CiteScore
1.20
自引率
0.00%
发文量
24
审稿时长
6-12 weeks
期刊介绍: Asian Biomedicine: Research, Reviews and News (ISSN 1905-7415 print; 1875-855X online) is published in one volume (of 6 bimonthly issues) a year since 2007. [...]Asian Biomedicine is an international, general medical and biomedical journal that aims to publish original peer-reviewed contributions dealing with various topics in the biomedical and health sciences from basic experimental to clinical aspects. The work and authorship must be strongly affiliated with a country in Asia, or with specific importance and relevance to the Asian region. The Journal will publish reviews, original experimental studies, observational studies, technical and clinical (case) reports, practice guidelines, historical perspectives of Asian biomedicine, clinicopathological conferences, and commentaries Asian biomedicine is intended for a broad and international audience, primarily those in the health professions including researchers, physician practitioners, basic medical scientists, dentists, educators, administrators, those in the assistive professions, such as nurses, and the many types of allied health professionals in research and health care delivery systems including those in training.
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