Niña Alyssa M Barroga, Van C Nguyen, Yuki Nakamura
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引用次数: 0
摘要
溶血磷脂酸酰基转移酶1（LPAT1）催化叶绿体中甘油脂从头开始生物合成的第二步。然而，基因敲除突变体的胚胎致死表型表明，LPAT1 在非光合生殖器官中的作用尚不清楚。lpat1-1杂合系的互交遗传表明，lpat1-1基因敲除突变体存在雌配子体缺陷。通过在种子发育过程中特异性抑制 LPAT1，我们发现 LPAT1 的抑制影响了颖果的生长和种子的生产。对LPAT1基因敲除株的甘油酯分析表明，成熟的颖果中磷脂酰胆碱（PC）含量明显下降，极性甘油酯的多不饱和度也发生了变化。在种子中，三酰甘油（TAG）的酰基组成发生了变化，但含量没有变化。这些结果表明，质体 LPAT1 在生殖生长和涉及 PC 和 TAG 的质体外甘油脂代谢中发挥着重要作用。
The role of lysophosphatidic acid acyltransferase 1 in reproductive growth of Arabidopsis thaliana.
Lysophosphatidic acid acyltransferase1 (LPAT1) catalyzes the second step of de novo glycerolipid biosynthesis in chloroplasts. However, the embryonic-lethal phenotype of the knockout mutant suggested an unknown role for LPAT1 in non-photosynthetic reproductive organs. Reciprocal genetic crossing of the lpat1-1 heterozygous line suggested a female gametophytic defect of the lpat1-1 knockout mutant. By suppressing LPAT1 specifically during seed development, we showed that LPAT1 suppression affected silique growth and seed production. Glycerolipid analysis of the LPAT1 knockdown lines revealed a pronounced decrease of phosphatidylcholine (PC) content in mature siliques along with an altered polyunsaturation level of the polar glycerolipids. In seeds, the acyl composition of triacylglycerol (TAG) was altered albeit not the content. These results indicate that plastidic LPAT1 plays an important role in reproductive growth and extraplastidic glycerolipid metabolism involving PC and TAG.
期刊介绍：
The Journal of Experimental Botany publishes high-quality primary research and review papers in the plant sciences. These papers cover a range of disciplines from molecular and cellular physiology and biochemistry through whole plant physiology to community physiology.
Full-length primary papers should contribute to our understanding of how plants develop and function, and should provide new insights into biological processes. The journal will not publish purely descriptive papers or papers that report a well-known process in a species in which the process has not been identified previously. Articles should be concise and generally limited to 10 printed pages.
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