BdMUTE 在模式草 Brachypodium distachyon 气孔发育过程中的双重作用。

IF 3.7 2区 生物学 Q1 DEVELOPMENTAL BIOLOGY
Development Pub Date : 2024-10-15 Epub Date: 2024-09-26 DOI:10.1242/dev.203011
Roxane P Spiegelhalder, Lea S Berg, Tiago D G Nunes, Melanie Dörr, Barbara Jesenofsky, Heike Lindner, Michael T Raissig
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引用次数: 0

摘要

禾本科植物在形态上形成了四细胞气孔,其中两个哑铃状的保卫细胞(GC)两侧是两个侧向附属细胞(SC)。这种新颖的形式使气孔能够快速打开和关闭,并实现植物与大气的高效气体交换。禾本科植物的 SC 形成需要移动 bHLH 转录因子 MUTE。然而,MUTE 是否以及如何调控 GC 的发育,以及 MUTE 的流动性是否是 SC 招募所必需的,目前还不清楚。在这里,我们通过转基因方法削弱了BdMUTE在新兴模式草Brachypodium distachyon中从GC到SC前体的移动能力。我们的数据表明,BdMUTE 移动性的降低严重影响了 GC 和 SC 发育的时空协调。此外,虽然 BdMUTE 在 GC 分裂定向中起着细胞自主作用,但 GC 的完整哑铃状形态发生需要 SC 招募。最后,叶片水平的气体交换测量表明,四细胞禾本科植物形态的剂量依赖性互补反映在气孔动力学的逐步生理互补上。总之,我们的工作揭示了禾本科植物 MUTE 在调节 GC 分裂方向和 SC 招募方面的双重作用,而 GC 的形态发生和禾本科植物气孔的快速动力学又需要这种作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dual role of BdMUTE during stomatal development in the model grass Brachypodium distachyon.

Grasses form morphologically derived, four-celled stomata, where two dumbbell-shaped guard cells (GCs) are flanked by two lateral subsidiary cells (SCs). This innovative form enables rapid opening and closing kinetics and efficient plant-atmosphere gas exchange. The mobile bHLH transcription factor MUTE is required for SC formation in grasses. Yet whether and how MUTE also regulates GC development and whether MUTE mobility is required for SC recruitment is unclear. Here, we transgenically impaired BdMUTE mobility from GC to SC precursors in the emerging model grass Brachypodium distachyon. Our data indicate that reduced BdMUTE mobility severely affected the spatiotemporal coordination of GC and SC development. Furthermore, although BdMUTE has a cell-autonomous role in GC division orientation, complete dumbbell morphogenesis of GCs required SC recruitment. Finally, leaf-level gas exchange measurements showed that dosage-dependent complementation of the four-celled grass morphology was mirrored in a gradual physiological complementation of stomatal kinetics. Together, our work revealed a dual role of grass MUTE in regulating GC division orientation and SC recruitment, which in turn is required for GC morphogenesis and the rapid kinetics of grass stomata.

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来源期刊
Development
Development 生物-发育生物学
CiteScore
6.70
自引率
4.30%
发文量
433
审稿时长
3 months
期刊介绍: Development’s scope covers all aspects of plant and animal development, including stem cell biology and regeneration. The single most important criterion for acceptance in Development is scientific excellence. Research papers (articles and reports) should therefore pose and test a significant hypothesis or address a significant question, and should provide novel perspectives that advance our understanding of development. We also encourage submission of papers that use computational methods or mathematical models to obtain significant new insights into developmental biology topics. Manuscripts that are descriptive in nature will be considered only when they lay important groundwork for a field and/or provide novel resources for understanding developmental processes of broad interest to the community. Development includes a Techniques and Resources section for the publication of new methods, datasets, and other types of resources. Papers describing new techniques should include a proof-of-principle demonstration that the technique is valuable to the developmental biology community; they need not include in-depth follow-up analysis. The technique must be described in sufficient detail to be easily replicated by other investigators. Development will also consider protocol-type papers of exceptional interest to the community. We welcome submission of Resource papers, for example those reporting new databases, systems-level datasets, or genetic resources of major value to the developmental biology community. For all papers, the data or resource described must be made available to the community with minimal restrictions upon publication. To aid navigability, Development has dedicated sections of the journal to stem cells & regeneration and to human development. The criteria for acceptance into these sections is identical to those outlined above. Authors and editors are encouraged to nominate appropriate manuscripts for inclusion in one of these sections.
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