Eun-Yeong Bok, Seung-Won Yi, Han Gyu Lee, Jae Kyeom Kim, Kangwook Lee, Seungmin Ha, Bumseok Kim, Young-Hun Jung, Sang-Ik Oh
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Functional and pathway enrichment analyses of the differentially expressed genes highlighted the pivotal roles of innate and adaptive immune responses, notably in pathways associated with Toll-like receptors, NIK/NF-κB signaling, cytokine signaling, and T cell proliferation. RT-qPCR-based validation using peripheral blood mononuclear cells provided additional insights into the immune system dynamics in response to ST infection, revealing the marked elevation of the interleukin (<i>IL</i>)<i>-15</i>, <i>IL-27</i>, and <i>CXCL10</i> levels being significantly elevated in ST-infected piglets. 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引用次数: 0
摘要
猪感染伤寒沙门氏菌(ST)对动物健康和食品安全构成了重大威胁;但人们对宿主免疫反应和病原体持续存在的复杂机制仍然知之甚少。为了填补这一知识空白,我们全面分析了感染 ST 的仔猪外周血转录组。我们进行了组织病理学评估、血液参数分析、先进的 RNA 测序技术和基于 RT-qPCR 的定量反转录 PCR(RT-qPCR)验证。感染后 2 天单核细胞计数的增加表明它有可能成为仔猪 ST 感染的血液学标志物。对差异表达基因的功能和通路富集分析强调了先天性免疫反应和适应性免疫反应的关键作用,尤其是在与 Toll 样受体、NIK/NF-κB 信号转导、细胞因子信号转导和 T 细胞增殖相关的通路中。使用外周血单核细胞进行的基于 RT-qPCR 的验证进一步揭示了免疫系统对 ST 感染的动态反应,发现 ST 感染仔猪的白细胞介素 (IL)-15、IL-27 和 CXCL10 水平明显升高。我们的综合分析强调了ST感染对仔猪的多方面影响,并为了解ST感染期间宿主与病原体的相互作用以及宿主免疫系统的作用提供了宝贵的见解。
Comprehensive analysis of a peripheral blood transcriptome signature in piglets infected with Salmonella Typhimurium: insight into immune responses
Salmonella Typhimurium (ST) infection in pigs poses a significant threat to animal health and food safety; the intricate mechanisms underlying host–immune responses and pathogen persistence remain poorly understood. To address this knowledge gap, we comprehensively analyzed the peripheral blood transcriptome in piglets infected with ST. We performed histopathological evaluation, blood parameter analysis, advanced RNA-sequencing techniques, and quantitative reverse transcription PCR (RT-qPCR)-based validation. The increasement in the monocyte counts at 2 days post-infection suggested its potential to serve as a hematological marker for ST infection in piglets. Functional and pathway enrichment analyses of the differentially expressed genes highlighted the pivotal roles of innate and adaptive immune responses, notably in pathways associated with Toll-like receptors, NIK/NF-κB signaling, cytokine signaling, and T cell proliferation. RT-qPCR-based validation using peripheral blood mononuclear cells provided additional insights into the immune system dynamics in response to ST infection, revealing the marked elevation of the interleukin (IL)-15, IL-27, and CXCL10 levels being significantly elevated in ST-infected piglets. Our comprehensive analysis underscores the multifaceted impact of ST infection on piglets and offers valuable insights into the host–pathogen interactions and the role of host immune system during ST infection.
期刊介绍:
Applied Biological Chemistry aims to promote the interchange and dissemination of scientific data among researchers in the field of agricultural and biological chemistry. The journal covers biochemistry and molecular biology, medical and biomaterial science, food science, and environmental science as applied to multidisciplinary agriculture.