A novel electrocatalyst based on NiO@C-dot nanocomposites for sensitive determination of ophthalmic drugs

This study proposed a novel electrochemical sensor for the sensitive measurement of the eye medication ofloxacin (OFX), which is based on a NiO@C-dot nanocomposite. The NiO@C-dot nanocomposite was synthesized using the sol-gel method, which was then applied to alter a glassy carbon electrode (GCE). With regard to OFX, the modified GCE shown outstanding electrochemical activity along with good sensitivity, selectivity, and stability. With OFX concentrations ranging from 5 µM to 975 µM, the NiO@C-dot/GCE showed a linear relationship with a sensitivity of 0.63994 µA/µM. The limit of detection (LOD) was 0.023 µM. The response to 50 µM OFX was measured, and the electrode was stored at 4°C after each test in order to evaluate the sensor's long-term stability and repeatability. After 20 days, the peak current response was still 97.89 % of its initial value. After 40 days of prolonged storage, 93.78 % of the electrode's original responsiveness was retained. The sensor showed a recovery rate of 97.37–99.70 % in all sample types when it was used to detect OFX in human urine, tap water, and food samples. These findings highlight the NiO@C-dot/GCE's outstanding selectivity, dependability, and longevity as solid platforms for OFX detection in complicated samples for a range of applications.