蜂胶通过靶向 MKK4 通路抑制特应性皮炎

IF 5 3区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
BioFactors Pub Date : 2024-08-20 DOI:10.1002/biof.2119
Ye-Ryeong Cho, Eui Jeong Han, Eun Heo, Arachchige Maheshika Kumari Jayasinghe, Jihyun Won, Soohwan Lee, Taegun Kim, Sung-Kuk Kim, Seokwon Lim, Soon Ok Woo, Gyoonhee Han, Wonku Kang, Ginnae Ahn, Sanguine Byun
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引用次数: 0

摘要

蜂胶是蜜蜂通过混合各种植物制成的天然树脂物质。蜂胶具有多种有益的生物活性,已被广泛认为是一种功能性食品。然而,食用蜂胶对特应性皮炎(AD)的治疗效果在很大程度上仍然未知。本研究旨在调查蜂胶对特应性皮炎的潜在疗效,并探索其活性化合物和直接分子靶标。在HaCaT角质细胞中,蜂胶抑制了TNF-α诱导的白细胞介素(IL)-6和IL-8的分泌。蜂胶还能减少趋化因子,如单核细胞趋化蛋白-1(MCP-1)和巨噬细胞衍生趋化因子(MDC),同时恢复屏障蛋白、丝胶蛋白和内卷蛋白的水平。蜂胶对类似AD的人类皮肤也有类似作用,抑制了AD标记物,恢复了屏障蛋白。在DNCB诱导的小鼠中,口服蜂胶可减轻AD症状,改善屏障功能,降低搔抓频率和经表皮失水(TEWL)。此外,蜂胶还能逆转小鼠背侧皮肤中AD相关标记物的mRNA水平。这些作用归因于咖啡酸苯乙酯(CAPE),它是通过比较蜂胶的主要成分而确定的活性化合物。机理研究显示,咖啡酸苯乙酯和蜂胶可直接选择性地靶向MKK4。总之,这些研究结果表明,蜂胶可用作治疗注意力缺失症的功能性食品剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Propolis suppresses atopic dermatitis through targeting the MKK4 pathway.

Propolis suppresses atopic dermatitis through targeting the MKK4 pathway.

Propolis is a natural resinous substance made by bees through mixing various plant sources. Propolis has been widely recognized as a functional food due to its diverse range of beneficial bioactivities. However, the therapeutic effects of consuming propolis against atopic dermatitis (AD) remain largely unknown. The current study aimed to investigate the potential efficacy of propolis against AD and explore the active compound as well as the direct molecular target. In HaCaT keratinocytes, propolis inhibited TNF-α-induced interleukin (IL)-6 and IL-8 secretion. It also led to a reduction in chemokines such as monocyte chemoattractant protein-1 (MCP-1) and macrophage-derived chemokine (MDC), while restoring the levels of barrier proteins, filaggrin and involucrin. Propolis exhibited similar effects in AD-like human skin, leading to the suppression of AD markers and the restoration of barrier proteins. In DNCB-induced mice, oral administration of propolis attenuated AD symptoms, improved barrier function, and reduced scratching frequency and transepidermal water loss (TEWL). In addition, propolis reversed the mRNA levels of AD-related markers in mouse dorsal skin. These effects were attributed to caffeic acid phenethyl ester (CAPE), the active compound identified by comparing major components of propolis. Mechanistic studies revealed that CAPE as well as propolis could directly and selectively target MKK4. Collectively, these findings demonstrate that propolis may be used as a functional food agent for the treatment of AD.

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来源期刊
BioFactors
BioFactors 生物-内分泌学与代谢
CiteScore
11.50
自引率
3.30%
发文量
96
审稿时长
6-12 weeks
期刊介绍: BioFactors, a journal of the International Union of Biochemistry and Molecular Biology, is devoted to the rapid publication of highly significant original research articles and reviews in experimental biology in health and disease. The word “biofactors” refers to the many compounds that regulate biological functions. Biological factors comprise many molecules produced or modified by living organisms, and present in many essential systems like the blood, the nervous or immunological systems. A non-exhaustive list of biological factors includes neurotransmitters, cytokines, chemokines, hormones, coagulation factors, transcription factors, signaling molecules, receptor ligands and many more. In the group of biofactors we can accommodate several classical molecules not synthetized in the body such as vitamins, micronutrients or essential trace elements. In keeping with this unified view of biochemistry, BioFactors publishes research dealing with the identification of new substances and the elucidation of their functions at the biophysical, biochemical, cellular and human level as well as studies revealing novel functions of already known biofactors. The journal encourages the submission of studies that use biochemistry, biophysics, cell and molecular biology and/or cell signaling approaches.
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