肯尼亚卡贾多县水样、牛粪和鸡粪样本中大肠弯曲杆菌和空肠弯曲杆菌病毒相关标记物的分子检测。

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BioMed Research International Pub Date : 2024-08-13 eCollection Date: 2024-01-01 DOI:10.1155/2024/4631351
Daniel W Wanja, Christine M Mbindyo, Paul G Mbuthia, Lilly C Bebora, Gabriel O Aboge
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引用次数: 0

摘要

弯曲杆菌是一种人畜共患的食源性病原体,通常与人类的肠胃炎和其他肠道外感染有关。本研究旨在确定弯曲杆菌中负责鞭毛蛋白运动蛋白 A (flaA)、弯曲杆菌粘附纤连蛋白 F (cadF)、弯曲杆菌侵袭抗原 B (ciaB) 和细胞致死膨胀毒素 (cdt) A (cdtA) 的毒力编码基因的遗传决定因素。利用聚合酶链式反应(PCR)和扩增片段测序技术,对肯尼亚卡贾多县早期研究中描述的 29 个大肠弯曲杆菌分离物(16 个来自牛、9 个来自鸡、4 个来自水样)和 74 个空肠弯曲杆菌分离物(38 个来自牛、30 个来自鸡、6 个来自水样)进行了检测,以确定是否存在毒力相关基因。毒力基因之间的相关性采用皮尔逊相关系数(R)法进行分析。在筛查出的 103 株弯曲杆菌中,89 株携带一个或多个毒力基因,总患病率为 86.4%。空肠弯曲菌株的多毒力发生率最高,为 64.9%(48/74),而大肠弯曲菌为 58.6%(17/29)。ciaB 和 flaA 基因是空肠弯曲菌(分别为 81.1% [60/74] 和 62.2% [46/74])和大肠杆菌(分别为 62.1%;18/29)中最常见的毒力基因。从鸡肉中分离出的弯曲杆菌含有最多的毒力编码基因。鸡和牛的空肠弯曲菌株分别含有比例最高的 cdtA 和 ciaB 基因。水样中的所有大肠杆菌菌株都含有 cadF 和 flaA 基因。研究结果进一步表明,cadF 和 flaA 之间存在明显的正相关性(R = 0.733)。来自牛、鸡和水的空肠弯曲杆菌和大肠弯曲杆菌菌株含有毒力标记,负责运动/定殖、侵袭、粘附和毒素产生,这表明它们在人类和牲畜弯曲杆菌病的发生中扮演着重要角色。牛、鸡和水样本被确定为毒性弯曲杆菌属的贮藏地,这凸显了它们对人类健康可能造成的风险。这些关于弯曲杆菌某些毒力基因的数据将有助于食品安全和公共卫生官员制定政策声明。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular Detection of Virulence-Associated Markers in Campylobacter coli and Campylobacter jejuni Isolates From Water, Cattle, and Chicken Faecal Samples From Kajiado County, Kenya.

Campylobacter is a zoonotic foodborne pathogen that is often linked with gastroenteritis and other extraintestinal infections in humans. This study is aimed at determining the genetic determinants of virulence-encoding genes responsible for flagellin motility protein A (flaA), Campylobacter adhesion to fibronectin F (cadF), Campylobacter invasion antigen B (ciaB) and cytolethal distending toxin (cdt) A (cdtA) in Campylobacter species. A total of 29 Campylobacter coli isolates (16 from cattle, 9 from chicken, and 4 from water samples) and 74 Campylobacter jejuni isolates (38 from cattle, 30 from chicken, and 6 from water samples) described in an earlier study in Kajiado County, Kenya, were examined for the occurrence of virulence-associated genes using polymerase chain reaction (PCR) and amplicon sequencing. The correlations among virulence genes were analyzed using Pearson's correlation coefficient (R) method. Among the 103 Campylobacter strains screened, 89 were found to harbour a single or multiple virulence gene(s), giving an overall prevalence of 86.4%. C. jejuni strains had the highest prevalence of multivirulence at 64.9% (48/74), compared to C. coli (58.6%, 17/29). The ciaB and flaA genes were the most common virulence genes detected in C. jejuni (81.1% [60/74] and 62.2% [46/74], respectively) and in C. coli (each at 62.1%; 18/29). Campylobacter isolates from chicken harboured the most virulence-encoding genes. C. jejuni strains from chicken and cattle harboured the highest proportions of the cdtA and ciaB genes, respectively. All the C. coli strains from water samples harboured the cadF and flaA genes. The results obtained further revealed a significant positive correlation between cadF and flaA (R = 0.733). C. jejuni and C. coli strains from cattle, chicken, and water harbour virulence markers responsible for motility/colonization, invasion, adherence, and toxin production, evoking their important role in campylobacteriosis development among humans and livestock. The identification of cattle, chicken, and water samples as reservoirs of virulent Campylobacter spp. highlights the possible risk to human health. These data on some virulence genes of Campylobacter will assist food safety and public health officials in formulating policy statements.

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来源期刊
BioMed Research International
BioMed Research International BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MEDICINE, RESEARCH & EXPERIMENTAL
CiteScore
6.70
自引率
0.00%
发文量
1942
审稿时长
19 weeks
期刊介绍: BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject areas.
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