MAPKKK28 在 MKK1-MPK1 级联的上游发挥作用,调节水稻的脱落酸反应。

IF 6 1区 生物学 Q1 PLANT SCIENCES
Ning Ren, Gang Zhang, Xiaokun Yang, Jing Chen, Lan Ni, Mingyi Jiang
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引用次数: 0

摘要

丝裂原活化蛋白激酶(MAPK)级联(MAPKK-MAPKK-MAPK)在生物和非生物胁迫反应以及脱落酸(ABA)信号传导中起着关键作用。先前的一项研究表明,ABA 激活的 MKK1-MPK1 级联在调节水稻的 ABA 反应和抗逆性方面至关重要。然而,MAPKK1-MPK1 级联在 ABA 信号传导中的上游特异性 MAPKKK 仍然未知。在这里,我们发现 MAPKKK28 是水稻 MEKK 家族中一个之前未表征的成员,它参与调控 ABA 反应,包括种子萌发、根系生长、气孔关闭以及对氧化应激和渗透胁迫的耐受性。我们发现 MAPKKK28 与 MKK1 直接相互作用并使其磷酸化。进一步分析表明,在 ABA 信号传导过程中,MKK1 和 MPK1 的激活都依赖于 MAPKKK28。遗传分析表明,MAPKKK28 在 MKK1-MPK1 级联的上游发挥作用,能积极调节 ABA 反应并增强对氧化和渗透胁迫的耐受性。这些结果不仅揭示了植物中一个新的完整的 MAPK 级联,而且揭示了它在 ABA 信号传导中的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
MAPKKK28 functions upstream of the MKK1-MPK1 cascade to regulate abscisic acid responses in rice.

The mitogen-activated protein kinase (MAPK) cascade (MAPKKK-MAPKK-MAPK) plays a critical role in biotic and abiotic stress responses and abscisic acid (ABA) signalling. A previous study has shown that the ABA-activated MKK1-MPK1 cascade is essential in regulating ABA response and stress tolerance in rice. However, the specific MAPKKK upstream of the MKK1-MPK1 cascade in ABA signalling remains unknown. Here, we identified that MAPKKK28, a previously uncharacterized member of the rice MEKK family, is involved in regulating ABA responses, including seed germination, root growth, stomatal closure, and the tolerance to oxidative stress and osmotic stress. We found that MAPKKK28 directly interacts with and phosphorylates MKK1. Further analysis indicated that the activation of both MKK1 and MPK1 depends on MAPKKK28 in ABA signalling. Genetic analysis revealed that MAPKKK28 functions upstream of the MKK1-MPK1 cascade to positively regulate ABA responses and enhance tolerance to oxidative and osmotic stress. These results not only reveal a new complete MAPK cascade in plants but also uncover its importance in ABA signalling.

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来源期刊
Plant, Cell & Environment
Plant, Cell & Environment 生物-植物科学
CiteScore
13.30
自引率
4.10%
发文量
253
审稿时长
1.8 months
期刊介绍: Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.
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