原地保存的大麦品种中预测的有害突变的差异性

IF 2 3区 农林科学 Q2 AGRONOMY
Crop Science Pub Date : 2024-08-20 DOI:10.1002/csc2.21325
Yong-Bi Fu
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引用次数: 0

摘要

要了解全球 1750 个基因库中 700 多万份植物种质长期保存所需的遗传成本,就必须了解保存的种质集合内部和之间有害突变的程度和变化。我们最近的研究揭示了预测对基因功能有害的多种突变,并对大麦、小麦、燕麦、大豆、玉米、芸苔属植物和向日葵种质库中每个有害基因座的突变负担进行了取样平均。在此,我们报告了对大麦(Hordeum vulgare L.)种质资源库中的九个品种进行 RNA-Seq 分析后发现的有害突变的程度和差异。结果发现,被预测为有害突变的单核苷酸多态性(SNPs)在数量和比例上存在显著差异(分别从 242 个到 314 个不等,平均为 270 个;从 0.00073 个到 0.00099 个不等,平均为 0.00086 个)。同样,每个有害基因座的平均样本突变负荷估计值也有很大差异,从 0.541 到 0.747 不等,平均值为 0.681。在预测的有害 SNP 比例和平均样本突变负荷估计值方面,栽培品种的平均值高于陆地栽培品种(分别为 0.00090 对 0.00082 和 0.695 对 0.663)。九个品种的有害碱基替换突变估计值(×10-8)从 0.313 到 0.406 不等,平均值为 0.349。这些收集范围内的研究结果有助于了解保存种质的遗传成本,并对长期种质管理和保护具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Variability in predicted deleterious mutations among barley accessions conserved ex situ

Variability in predicted deleterious mutations among barley accessions conserved ex situ

Understanding the genetic cost for long-term conservation of more than 7 million plant germplasm accessions in 1750 genebanks worldwide requires knowledge about the extent and variation of deleterious mutations within and among conserved germplasm collections. Our recent study revealed a wide range of mutations predicted to be deleterious to gene function and averaged sample-wise mutation burden per deleterious locus across barley, wheat, oat, soybean, maize, rapa, and sunflower germplasm collections. Here we report the extent and variation of predicted deleterious mutations among nine accessions of a barley (Hordeum vulgare L.) collection from an RNA-Seq analysis of 16 individual samples of each accession. The assayed accessions were found to vary significantly in the number and proportion of single nucleotide polymorphisms (SNPs) predicted to be deleterious mutations (ranging from 242 to 314 with a mean of 270 and from 0.00073 to 0.00099 with a mean of 0.00086, respectively). Similarly, a significant variation was also observed in the averaged sample-wise mutation burden estimates per deleterious locus, ranging from 0.541 to 0.747 with a mean of 0.681. Cultivar accessions had higher averages in the proportions of the predicted deleterious SNPs and in the averaged sample-wise mutation burden estimates than landrace accessions (0.00090 vs. 0.00082 and 0.695 vs. 0.663, respectively). The estimates of deleterious base-substitution mutations (×10−8) for the nine accessions varied from 0.313 to 0.406 with a mean of 0.349. These within-collection findings are useful for understanding the genetic cost in conserved germplasm and have implications for long-term germplasm management and conservation.

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来源期刊
Crop Science
Crop Science 农林科学-农艺学
CiteScore
4.50
自引率
8.70%
发文量
197
审稿时长
3 months
期刊介绍: Articles in Crop Science are of interest to researchers, policy makers, educators, and practitioners. The scope of articles in Crop Science includes crop breeding and genetics; crop physiology and metabolism; crop ecology, production, and management; seed physiology, production, and technology; turfgrass science; forage and grazing land ecology and management; genomics, molecular genetics, and biotechnology; germplasm collections and their use; and biomedical, health beneficial, and nutritionally enhanced plants. Crop Science publishes thematic collections of articles across its scope and includes topical Review and Interpretation, and Perspectives articles.
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