将循环 microRNA-126-3p 鉴定为冠状动脉钙化的新生物标记物。

IF 2.3 Q2 MEDICINE, GENERAL & INTERNAL
SAGE Open Medicine Pub Date : 2024-08-18 eCollection Date: 2024-01-01 DOI:10.1177/20503121241272646
Xia Zhang, Mengmeng Zhu, Peng Zeng, Mingxiu Guan, Hongyu Zhang, Shaohua Duan, Heli Huang, Yulian Liu, Hongliang Cong, Yuanli Chen
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引用次数: 0

摘要

目的:包括microRNA-126-3p在内的几种循环microRNA已被确定为心血管疾病的诊断和预后生物标志物。然而,microRNA-126-3p 是否是冠状动脉钙化的独立风险预测因子尚不清楚:在这项前瞻性单中心研究中,我们收集了冠状动脉粥样硬化患者(54 人)、冠状动脉钙化患者(33 人)和对照组(56 人)的血液样本。用 TRIzol 试剂从血浆和血细胞中提取总 RNA。微RNA-126-3p水平通过实时定量聚合酶链反应(RT-PCR)测定:结果:与冠状动脉粥样硬化患者或对照组相比,冠状动脉钙化患者的 microRNA-126-3p 水平明显升高。经冠状动脉造影诊断为 2 级钙化的中度钙化患者的 microRNA-126-3p 表达量最高。年龄、静脉中microRNA-126-3p的表达、高血压和糖尿病对冠状动脉钙化的发生有显著影响,其中糖尿病和静脉中microRNA-126-3p的表达是冠状动脉钙化的独立危险因素:综上所述,本研究的数据表明,循环 microRNA-126-3p 可能是冠状动脉钙化的一种新型无创生物标志物。调节microRNA-126-3p的表达可能是诊断和治疗心血管疾病,尤其是冠状动脉钙化的有效且有前景的策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification of circulating microRNA-126-3p as a new biomarker for coronary artery calcification.

Objective: Several circulating microRNAs, including microRNA-126-3p, have been identified as diagnostic and prognostic biomarker of cardiovascular disease. However, whether microRNA-126-3p is an independent risk predictor for coronary artery calcification is unclear.

Methods: In this prospective single-center study, we collected blood samples from coronary artery atherosclerosis patients (n = 54), patients with coronary artery calcification (n = 33) and controls (n = 56). Total RNA was extracted from plasma and blood cells with TRIzol reagents. The microRNA-126-3p level was determined via quantitative real-time polymerase chain reaction (RT-PCR).

Results: MicroRNA-126-3p levels were significantly increased in patients with coronary artery calcification than in coronary artery atherosclerosis patients or controls. The highest expression of microRNA-126-3p was observed in patients with moderate calcification who were diagnosed with Grade 2 calcification by coronary angiography. Age, microRNA-126-3p expression in veins, hypertension and diabetes significantly influence the occurrence of coronary artery calcification, among which diabetes and venous microRNA-126-3p expression were found to be independent risk factors for coronary artery calcification.

Conclusions: Taken together, the data in this study suggest that circulating microRNA-126-3p may be a novel noninvasive biomarker for coronary artery calcification. Regulating microRNA-126-3p expression may be an effective and promising strategy for the diagnosis and treatment of cardiovascular diseases, especially coronary artery calcification.

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来源期刊
SAGE Open Medicine
SAGE Open Medicine MEDICINE, GENERAL & INTERNAL-
CiteScore
3.50
自引率
4.30%
发文量
289
审稿时长
12 weeks
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