包裹在阿贝摩斯多糖颗粒中的纳米粒子作为结肠靶向方法。

IF 3 4区医学 Q2 CHEMISTRY, APPLIED

Journal of microencapsulation Pub Date : 2024-11-01 Epub Date: 2024-08-20 DOI:10.1080/02652048.2024.2390951

Akshita Arora, Anshul Sharma, Shamsher Singh, Rajveer Singh, Amrinder Singh, Dipti Kakkar, Nitin Sharma

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引用次数: 0

摘要

目的本文探讨了美沙拉嗪载体纳米颗粒（MLZ-NPs）包封在阿贝莫斯植物多糖颗粒（MLZ-NPs-Pellets）中治疗溃疡性结肠炎的应用：制备并评估了MLZ-NPs的直径、PDI和包封效率。在 Caco-2 细胞上进行了体外药效研究。将 MLZ-NPs 包裹在多糖中形成 MLZ-NPs-Pellets，并对其在动物体内的疗效和在人类志愿者体内的靶向效率进行表征：优化批次的 MLZ-NPs 的直径、PDI、zeta 电位和包封效率分别为 145.42 ± 6.75 nm、0.214 ± 0.049、-31.63 mV 和 77.65 ± 2.33（%w/w）。用 MLZ-NPs 处理 Caco-2 细胞后，ROS、超氧化物和 NF-kβ 均得到了很好的控制。体内数据显示，口服 MLZ-NPs-Pellets 后，DSS 诱导的小鼠模型的一些参数（体重、结肠长度、脂质过氧化物酶和谷胱甘肽）显著恢复。伽马闪烁成像显示，该制剂可在 600 分钟内有效靶向结肠：结论：MLZ-NPs-颗粒可有效用于微生物触发的结肠靶向疗法，以治疗溃疡性结肠炎。

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查看原文本刊更多论文

Nanoparticles encapsulated in Abelmoschus esculentus polysaccharide-based pellets as colon targeting approach.

Aim(s): This article explores the application of mesalazine-loaded nanoparticles (MLZ-NPs) encapsulated in Abelmoschus esculentus plant polysaccharide-based pellets (MLZ-NPs-Pellets) for ulcerative colitis.

Methods: MLZ-NPs were prepared and evaluated for diameter, PDI, and entrapment efficiency. In-vitro efficacy study was conducted on Caco-2 cells. MLZ-NPs were encapsulated in polysaccharides to form MLZ-NPs-Pellets and characterised for efficacy in animals and targeting efficiency in human volunteers.

Results: Optimised batch of MLZ-NPs were characterised for diameter, PDI, zeta potential and entrapment efficiency which was found to be 145.42 ± 6.75 nm, 0.214 ± 0.049, -31.63 mV and 77.65 ± 2.33(%w/w) respectively. ROS, superoxide and NF-kβ were well controlled in Caco-2 cells when treated with MLZ-NPs. In-vivo data revealed that some parameters (body weight, colon length, lipid peroxidase, and glutathione) recovered significantly in the DSS-induced mice model treated with oral MLZ-NPs-Pellets. Gamma scintigraphy revealed that the formulation can effectively target the colon within 600 min.

Conclusion: MLZ-NPs-Pellets can be effectively used for microbial-triggered colon targeting approach in treating ulcerative colitis.

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来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.