Nicolas Dierckxsens, Kosei Watanabe, Yongkai Tan, Aki Masunaga, Michael J Mansfield, Jiashun Miao, Nicholas M Luscombe, Charles Plessy
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引用次数: 0
摘要
Oikopleura dioica 是一种广泛分布于全球海洋水域的浮游鳞栉水母（Appendicularia class）。我们采用单分子 PacBio Hi-Fi 方法对从日本冲绳采集的一只雄性个体的基因组进行了测序，并用 NOVOLoci 进行了组装。有丝分裂基因组长 39,268 bp，其中有一个约 22,000 bp 的大型控制区。我们对蛋白 atp6、cob、cox1、cox2、cox3、nad1、nad4 和 nad5 进行了注释，还发现了一个与任何已知基因都不匹配的开放阅读框。这项研究标志着首次完整地组装了附肢动物的有丝分裂基因组，并揭示了A和T同源聚合体累计占其长度的近一半。该参考序列将成为环境 DNA 和系统发育研究的宝贵财富。
Tracing Homopolymers in Oikopleura dioica's Mitogenome.
Oikopleura dioica is a planktonic tunicate (Appendicularia class) found extensively across the marine waters of the globe. The genome of a single male individual collected from Okinawa, Japan was sequenced using the single-molecule PacBio Hi-Fi method and assembled with NOVOLoci. The mitogenome is 39,268 bp long, featuring a large control region of around 22,000 bp. We annotated the proteins atp6, cob, cox1, cox2, cox3, nad1, nad4, and nad5, and found one more open reading frame that did not match any known gene. This study marks the first complete mitogenome assembly for an appendicularian, and reveals that A and T homopolymers cumulatively account for nearly half of its length. This reference sequence will be an asset for environmental DNA and phylogenetic studies.
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