通过质子核磁共振量化高密度脂蛋白颗粒数量:不要相信数字。

IF 3.8 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Current opinion in lipidology Pub Date : 2024-10-01 Epub Date: 2024-08-19 DOI:10.1097/MOL.0000000000000948
Tomas Vaisar, Jay Heinecke
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引用次数: 0

摘要

审查目的:质子核磁共振(NMR)可快速评估生物样本中脂蛋白的浓度和大小。它对量化高密度脂蛋白(HDL)尤其有用,因为高密度脂蛋白的颗粒大小和浓度各不相同。我们对核磁共振在量化高密度脂蛋白亚类方面的优势和局限性进行了严格的审查:最近使用 NMR 进行的研究揭示了高密度脂蛋白在各种疾病中的作用,包括残余心血管风险和宿主易感性。然而,准确量化高密度脂蛋白颗粒的数量、大小和浓度(HDL-P)仍然是一项挑战。在估计高密度脂蛋白种类的丰度和脂蛋白 A-I (APOA1) 与高密度脂蛋白颗粒的比率方面,核磁共振与凝胶电泳、离子迁移率分析和大小排阻色谱等其他方法之间存在差异。然而,核磁共振定量 HDL-P 的结果与其他独立方法之间的不一致性妨碍了对核磁共振结果的解释。由于质子 NMR 显然无法准确量化 HDL 的大小和浓度,此类研究与 HDL 生物学的相关性面临挑战。这限制了我们对 NMR 确定的 HDL-P 的病理生理学意义的理解,尤其是在确定心血管疾病(CVD)风险方面。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Quantification of high-density lipoprotein particle number by proton nuclear magnetic resonance: don't believe the numbers.

Purpose of review: Proton nuclear magnetic resonance (NMR) can rapidly assess lipoprotein concentrations and sizes in biological samples. It may be especially useful for quantifying high-density lipoprotein (HDL), which exhibits diverse particle sizes and concentrations. We provide a critical review of the strengths and limitations of NMR for quantifying HDL subclasses.

Recent findings: Recent studies using NMR have shed light on HDL's role in various disorders, ranging from residual cardiovascular risk to host susceptibility to infection. However, accurately quantifying HDL particle number, size, and concentration (HDL-P) remains a challenge. Discrepancies exist between NMR and other methods such as gel electrophoresis, ion mobility analysis and size-exclusion chromatography in estimating the abundance of HDL species and the ratio of apolipoprotein A-I (APOA1) to HDL particles.

Summary: NMR is a low-cost method for quantifying HDL-P that is readily applicable to clinical and translational studies. However, inconsistencies between the results of NMR quantification of HDL-P and other independent methods hinder the interpretation of NMR results. Because proton NMR apparently fails to accurately quantify the sizes and concentrations of HDL, the relevance of such studies to HDL biology poses challenges. This limits our understanding of pathophysiological implications of HDL-P as determined by NMR, particularly in determining cardiovascular disease (CVD) risk.

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来源期刊
Current opinion in lipidology
Current opinion in lipidology 医学-内分泌学与代谢
CiteScore
6.70
自引率
4.50%
发文量
64
审稿时长
6-12 weeks
期刊介绍: With its easy-to-digest reviews on important advances in world literature, Current Opinion in Lipidology offers expert evaluation on a wide range of topics from six key disciplines including nutrition and metabolism, genetics and molecular biology, and hyperlipidaemia and cardiovascular disease. Published bimonthly, each issue covers in detail the most pertinent advances in these fields from the previous year. This is supplemented by a section of Bimonthly Updates, which deliver an insight into new developments at the cutting edge of the disciplines covered in the journal.
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