氨基酸表面改性生物玻璃:骨组织工程的候选生物材料1。

IF 2 3区 工程技术 Q2 ANATOMY & MORPHOLOGY
Yasin Özkabadayı, Mustafa Türk, Ali Kumandaş, Siyami Karahan
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引用次数: 0

摘要

生物玻璃是由不同比例的氧化钠、氧化钙、二氧化硅和磷组成的固体材料,已用于骨组织工程。人们一直在努力改善生物玻璃的表面特性,以提高其生物相容性和性能。本研究的目的是用由精氨酸、天冬氨酸、苯丙氨酸、半胱氨酸、组氨酸和赖氨酸组成的氨基酸混合物改性生物玻璃表面,研究其表面特性,并评估其在体外和体内的性能和生物相容性。未经处理的生物玻璃、在模拟体液(SBF)中保存的生物玻璃和改性生物玻璃被用于进一步评估。通过傅立叶变换红外分析和扫描电镜分析确认表面改性后,扫描电镜还显示了 MC3T3-E1 前成骨细胞在表面的粘附情况。改性后的生物玻璃在比色法中的 ALP 活性、茜素红染色中的钙累积率、Annexin-V/PI 染色中的细胞凋亡率和坏死率都有明显提高。改性生物玻璃在 MTT 试验中的细胞存活率较高,在微核试验(OECD 487)中没有遗传毒性,这进一步证实了它在体外的生物相容性。大鼠胫骨缺损模型的结果显示,与未经处理的阴性对照组相比,所有生物玻璃处理的骨愈合得分都明显更高。然而,与其他生物玻璃处理方法相比,改性生物玻璃的骨愈合效果明显更好,尤其是在第一和第二个月。因此,对生物玻璃进行氨基酸表面改性可改善其表面生物相容性和成骨性能,从而使氨基酸改性生物玻璃成为骨组织工程的更佳候选材料。研究亮点:用氨基酸对生物玻璃进行表面改性有助于生物玻璃与组织的相互作用,改善细胞附着。改性生物玻璃可提高体外 Alp 活性和钙积累,并通过支持细胞适应性对细胞行为产生积极影响。生物玻璃在体内具有成骨潜能,尤其是在骨愈合早期。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Amino acid surface modified bioglass: A candidate biomaterial for bone tissue engineering1.

Bioglasses are solid materials consisted of sodium oxide, calcium oxide, silicon dioxide and phosphorus in various proportions and have used in bone tissue engineering. There have been ongoing efforts to improve the surface properties of bioglasses to increase biocompatibility and performance. The aim of the present study is to modify the bioglass surface with an amino acid mixture consisting of arginine, aspartic acid, phenylalanine, cysteine, histidine and lysine, to characterize the surface, and to evaluate the performance and biocompatibility in vitro and in vivo. The untreated bioglass, bioglass kept in simulated body fluid (SBF), and modified bioglass were used in further evaluation. After confirmation of the surface modification with FT-IR analyses and SEM analyses, MC3T3-E1 preosteoblasts adhesion on the surface was also revealed by SEM. The modified bioglass had significantly higher ALP activity in colorimetric measurement, rate of calcium accumulations in Alizarin red s staining, lower rate of cell death in Annexin-V/PI staining to determine apoptosis and necrosis. Having higher cell viability rate in MTT test and absence of genotoxicity in micronucleus test (OECD 487), the modified bioglass was further confirmed for biocompatibility in vitro. The results of the rat tibial defect model revealed that the all bioglass treatments had a significantly better bone healing score compared to the untreated negative control. However, the modified bioglass exhibited significantly better bone healing efforts especially during the first and the second months compared to the other bioglass treatment treatments. As a result, the amino acid surface modification of bioglasses improves the surface biocompatibility and osteogenic performance that makes the amino acid modified bioglass a better candidate for bone tissue engineering. RESEARCH HIGHLIGHTS: Bioglass surface modification with amino acids contributes to bioglass-tissue interaction with an improved cell attachment. Modified bioglass increases in vitro Alp activity and calcium accumulation, and also positively affects cell behavior by supporting cell adaptation. Bioglass exerts osteogenic potential in vivo especially during early bone healing.

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来源期刊
Microscopy Research and Technique
Microscopy Research and Technique 医学-解剖学与形态学
CiteScore
5.30
自引率
20.00%
发文量
233
审稿时长
4.7 months
期刊介绍: Microscopy Research and Technique (MRT) publishes articles on all aspects of advanced microscopy original architecture and methodologies with applications in the biological, clinical, chemical, and materials sciences. Original basic and applied research as well as technical papers dealing with the various subsets of microscopy are encouraged. MRT is the right form for those developing new microscopy methods or using the microscope to answer key questions in basic and applied research.
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