评估免疫检查点 TIM-3 和 PD-1、抗炎细胞因子 IL-10 和 TGF-β 以及慢性自发性荨麻疹的疾病活动。

IF 1.5 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Hadi Sadeghi, Javad Ghaffari, Javad Rajabi, Monireh Golpour, Torsten Zuberbier, Sadegh Fattahi, Hossein Asgarian-Omran, Alireza Rafiei
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引用次数: 0

摘要

慢性自发性荨麻疹(CSU)是一种由肥大细胞产生炎症介质引起的皮肤病。免疫检查点受体,如程序死亡-1(PD-1)和T细胞免疫球蛋白和粘蛋白结构域3(TIM-3),对许多自身免疫性和过敏性疾病的病理生理学至关重要。本研究旨在调查 CSU 患者体内 PD-1 和 TIM-3 的表达及其与抗炎细胞因子(TGF-β 和 IL-10)的关系。本研究使用了 CSU 患者和健康人的外周血单核细胞(PBMC),并使用荨麻疹活动评分 7(UAS7)来评估疾病的严重程度。采用基于 TaqMan 的 RT-PCR 技术评估 TIM-3 和 PD-1 以及抗炎细胞因子转化生长因子-β(TGF-β)和 IL-10 的表达。TGF-β和IL-10的蛋白浓度也是通过酶联免疫吸附法测定的。研究了 TIM-3 和 PD-1 以及 TGF- β 和 IL-10 的表达与疾病严重程度之间的关系。结果显示,CSU 患者的 PD-1 mRNA 表达明显增加(P
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of the Immune Checkpoints, TIM-3 and PD-1, as well as Anti-Inflammatory Cytokines IL-10, and TGF-β along with Diseases Activity in Chronic Spontaneous Urticaria.

Chronic spontaneous urticaria (CSU) is a skin disease caused by mast cells that produce inflammatory mediators. Immune checkpoint receptors such as program death-1 (PD-1) and T-cell immunoglobulin and mucin domain 3 (TIM-3) are essential for the pathophysiology of many autoimmune and allergic diseases. The aim of this study was to investigate the expression of PD-1 and TIM-3 in CSU patients and their relationship to the anti-inflammatory cytokines (TGF-β and IL-10). In the current study, peripheral blood mononuclear cells (PBMCs) from CSU patients and healthy individuals were used and the Urticaria Activity Score 7 (UAS7) was used to assess disease severity. TaqMan-based RT-PCR was used to assess the expression of TIM-3 and PD-1 as well as the anti-inflammatory cytokines transforming growth factor-β (TGF-β) and IL-10. The protein concentrations of TGF-β and IL-10 were also measured by ELISA. The relationship between the expression of TIM-3 and PD-1 as well as TGF- β and IL-10 and the severity of the disease was investigated. The results showed that PD-1 mRNA expression was significantly increased in CSU patients (P<0.0001), while TGF- β and IL-10 levels were higher in CSU patients, but this difference was not significant (p=0.638, p= 0.798). The increase in protein level of IL-10 was significant (P<0.0001). There was also a positive correlation between the expression of PD-1 and TGF- β molecules and disease activity (P=0.0043, P=0.0018). In conclusion, the study found that the immune system expresses inhibitory molecules and anti-inflammatory cytokines to control disease severity. The higher expression of PD-1 molecules and IL-10 is associated with disease severity, suggesting that the immune system is trying to control inflammation and reduce disease severity.

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来源期刊
CiteScore
3.60
自引率
0.00%
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0
期刊介绍: The International Journal of Molecular and Cellular Medicine (IJMCM) is a peer-reviewed, quarterly publication of Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran. The journal covers all cellular & molecular biology and medicine disciplines such as the genetic basis of disease, biomarker discovery in diagnosis and treatment, genomics and proteomics, bioinformatics, computer applications in human biology, stem cells and tissue engineering, medical biotechnology, nanomedicine, cellular processes related to growth, death and survival, clinical biochemistry, molecular & cellular immunology, molecular and cellular aspects of infectious disease and cancer research. IJMCM is a free access journal. All open access articles published in IJMCM are distributed under the terms of the Creative Commons Attribution CC BY. The journal doesn''t have any submission and article processing charges (APCs).
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