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引用次数: 0
摘要
诺如病毒（NoV）是引起急性肠胃炎的主要病原体，给全世界带来了沉重的社会经济负担。本研究从马尾藻（Sargassum fusiforme，S. fusiforme）中分离纯化出五种多糖组分，标记为 pSFP-1-5。体外实验表明，pSFP-5 能显著阻止 A、B 和 H 型组织血型抗原（HBGAs）与 NoV GII.4 病毒样颗粒（NoV GII.4 VLPs）的结合。此外，体内实验显示，pSFP-5 能有效减少 NoV 在牡蛎中的积累，这表明 pSFP-5 能降低食用牡蛎感染 NoV 的风险。透射电子显微镜结果显示，经过 pSFP-5 处理后，NoV GII.4 VLPs 的外观发生了变化，这表明 pSFP-5 可能通过改变病毒颗粒的形态结构，使其无法与 HBGAs 结合，从而达到抗病毒的目的。本研究结果表明，pSFP-5 可能是一种有效的抗野病毒物质，可用作潜在的抗野病毒药物成分。
Inhibition of Norovirus GII.4 binding to HBGAs by Sargassum fusiforme polysaccharide.
Norovirus (NoV) is the main pathogen that causes acute gastroenteritis and brings a heavy socio-economic burden worldwide. In this study, five polysaccharide fractions, labeled pSFP-1-5, were isolated and purified from Sargassum fusiforme (S. fusiforme). In vitro experiments demonstrated that pSFP-5 significantly prevented the binding of type A, B and H histo-blood group antigens (HBGAs) to NoV GII.4 virus-like particles (NoV GII.4 VLPs). In addition, in vivo experiments revealed that pSFP-5 was effective in reducing the accumulation of NoV in oysters, indicating that pSFP-5 could reduce the risk of NoV infection from oyster consumption. The results of transmission electron microscopy showed that the appearance of NoV GII.4 VLPs changed after pSFP-5 treatment, indicating that pSFP-5 may achieve antiviral ability by altering the morphological structure of the viral particles so that they could not bind to HBGAs. The results of the present study indicate that pSFP-5 may be an effective anti-NoV substance and can be used as a potential anti-NoV drug component.
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