海底真菌 Schizophyllum commune 15R-5-F01 对甲基红的降解:效率、途径和产物毒性。

IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
3 Biotech Pub Date : 2024-09-01 Epub Date: 2024-08-15 DOI:10.1007/s13205-024-04037-z
Hui Xu, Hong-Ye Zheng, Chang-Hong Liu
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引用次数: 0

摘要

合成染料因其复杂的结构和对微生物降解的抗性而对环境构成严重威胁。在含有 100 mg L-1 甲基红的培养基中,S. commune 15R-5-F01 真菌在 3 小时内对甲基红的降解效率超过 96%。该真菌表现出对各种环境条件的适应性,包括不同的 pH 值、温度、氧气浓度、盐度和重金属。S. commune 15R-5-F01 能够反复循环还原甲基红,持续降解时间最少为 6 个循环。它的最大甲基红生物降解能力至少为 558 毫克 g-1 干菌丝体,生物吸附能力为 57 毫克 g-1。气相色谱-质谱分析证实,甲基红被还原成 N,N-二甲基对苯二胺和 2-氨基苯甲酸。酶活性测定表明,木质素过氧化物酶、木质素酶和锰过氧化物酶参与了生物降解过程。对东亚小麦、大米和伞形木葡萄种子进行的植物毒性测试表明,与甲基红相比,降解产物的毒性有所降低。这项研究将 S. commune 15R-5-F01 确定为可持续降解工业废水中合成染料的可行候选物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Methyl Red degradation by a subseafloor fungus <i>Schizophyllum commune</i> 15R-5-F01: efficiency, pathway, and product toxicity.

Methyl Red degradation by a subseafloor fungus Schizophyllum commune 15R-5-F01: efficiency, pathway, and product toxicity.

Synthetic dyes pose a significant environmental threat due to their complex structures and resistance to microbial degradation. S. commune 15R-5-F01 exhibited over 96% degradation efficiency of Methyl Red in a medium with 100 mg L-1 Methyl Red within 3 h. The fungus demonstrated adaptability to various environmental conditions, including different pH levels, temperatures, oxygen concentrations, salinity, and heavy metals. S. commune 15R-5-F01 is capable of achieving repeated cycles of Methyl Red reduction with sustained degradation duration minimum of 6 cycles. It showed a maximum Methyl Red biodegradation capacity of at least 558 mg g-1 dry mycelia and a bioadsorption capacity of 57 mg g-1. Gas chromatography-mass spectrometry analysis confirmed the azo reduction of Methyl Red into N,N-dimethyl-p-phenylenediamine and 2-aminobenzoic acid. Enzymatic activity assays indicated the involvement of lignin peroxidases, laccases, and manganese peroxidase in the biodegradation process. Phytotoxicity tests on Triticum eastivum, Oryza sativa, and Vigna umbellata seeds revealed reduced toxicity of the degradation products compared to Methyl Red. This study identifies S. commune 15R-5-F01 as a viable candidate for the sustainable degradation of synthetic dyes in industrial wastewater.

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来源期刊
3 Biotech
3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
6.00
自引率
0.00%
发文量
314
期刊介绍: 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.
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