家猫在超声波引导下成功拾取卵子(OPU)并随后生产体外胚胎

IF 2.4 2区 农林科学 Q3 REPRODUCTIVE BIOLOGY
Andrea Priego-González , Ana Munoz-Maceda , Joaquín Cerdeira-Lozano , Hernán Fominaya , Manuel Fuertes-Recuero , Gustavo Ortiz-Díez , Manuel Gardoqui Arias , Eduardo R.S. Roldan , María Jesús Sánchez-Calabuig
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引用次数: 0

摘要

取卵术(OPU)是一种微创技术,广泛用于牛和母马的卵母细胞提取,包括超声引导下的卵泡穿刺。实践证明,在同一雌性动物身上重复使用这种技术是安全的,不会影响其生殖健康,因此无论其生殖状况如何,都可以取回卵母细胞。通过 OPU 获得的卵母细胞随后可用于辅助生殖技术(ART)的体外胚胎生产(IVP),或冷冻保存在生物库中,以备将来使用。传统上,在家养猫科动物和野生猫科动物体内实施的微创技术是腹腔镜取卵术(LOPU)。本研究旨在探讨在家猫体内使用超声引导取卵术(OPU)是否安全有效。在最初的一系列体外实验(n = 92 个卵巢,n = 434 个卵母细胞)中,研究人员探讨了不同抽吸压力对卵母细胞采集的影响。这些实验确定 43 mmHg 为最佳抽吸压力,可获得最高的回收率、良好的成熟度和囊胚率。随后,通过 OPU 提取了 16 个 I 级和 II 级卵母细胞,并通过卵巢切除和切片提取了 101 个卵母细胞。每种技术获得的 16 个卵母细胞都进行了体外成熟(IVM)和体外受精(IVF)。从每组(OPU 和切片)中挑选出 14 个推定合子进行体外培养(IVC),两组的卵裂率分别为 57.1 % 和 64.2 %,畸形率分别为 28.5 %,囊胚率分别为 7.14 % 和 14.2 %。激素刺激方案的耐受性良好,未发现任何不良反应。此外,在手术后进行的卵巢切除术中也没有出现并发症。在家猫身上使用这种技术在安全性、可复制性和侵入性方面迈出了一大步,为在野生猫科动物身上应用这种技术提供了宝贵的范例。要验证这些令人鼓舞的发现,还需要进行更多动物参与的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Successful ultrasound-guided ovum pick-up (OPU) and subsequent in vitro embryo production in a domestic cat

Ovum Pick Up (OPU) is a minimally invasive technique widely used in cattle and mares for oocyte retrieval, involving ultrasound-guided puncture of ovarian follicles. It has been demonstrated that this technique is safe for its repeated use in the same female without affecting her reproductive health, allowing for the retrieval of oocytes in individuals regardless of their reproductive status. The oocytes obtained through OPU can subsequently be used for in vitro embryo production (IVP) using assisted reproductive techniques (ARTs) or be cryopreserved in biobanks for their future use. Traditionally, the minimally invasive technique of choice performed in vivo in domestic and wild felines was LOPU (laparoscopic-guided ovum pick up). The present study was designed to explore if ultrasound-guided OPU in the domestic cat is safe and effective. In an initial series of ex vivo experiments (n = 92 ovaries, n = 434 oocytes), the effect of different aspiration pressures for oocyte collection was explored. These experiments identified 43 mmHg as the optimal aspiration pressure, resulting in the highest recovery rate and a favorable maturation and blastocyst rate. Subsequently, 16 grade I and II oocytes were retrieved by OPU and 101 oocytes were retrieved following ovariectomy and slicing. Sixteen oocytes obtained with each technique were subjected to in vitro maturation (IVM) and in vitro fertilization (IVF). A total of 14 presumptive zygotes were selected for in vitro culture (IVC) from each group (OPU and slicing), obtaining a cleavage rate of 57.1 % and 64.2 %, a morula rate of 28.5 % in both groups, and a blastocyst rate of 7.14 % and 14.2 % respectively. The hormonal stimulation protocol was well-tolerated, with no adverse effects observed. Moreover, no complications arose during the ovariectomy performed post-OPU. The use of this technique in domestic cats represents a significant step forward in terms of safety, replicability, and invasiveness, serving as a valuable model for its application in wild felids species. Additional research involving a greater number of animals is required to validate these encouraging findings.

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来源期刊
Theriogenology
Theriogenology 农林科学-生殖生物学
CiteScore
5.50
自引率
14.30%
发文量
387
审稿时长
72 days
期刊介绍: Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.
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