单细胞 RNA 序列分析揭示了人巨细胞病毒感染人源化小鼠体内免疫细胞的深刻反应。

IF 5.5 3区 医学 Q1 Medicine
An Wang , Xiao-Xu Zhu , Yuanyuan Bie , Bowen Zhang , Wenting Ji , Jing Lou , Muhan Huang , Xi Zhou , Yujie Ren
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引用次数: 0

摘要

人类巨细胞病毒(HCMV)是一种常见的疱疹病毒,可持续感染全球大部分人口。尽管宿主具有强大的免疫反应,但 HCMV 仍能复制、逃避宿主的防御,并通过开发多种免疫调节策略在整个生命周期内建立潜伏期,因此对 HCMV 感染与宿主反应之间相互作用的研究尤为重要。HCMV 有严格的宿主特异性,会专门感染人类。因此,对 HCMV 的体内研究大多依赖于临床样本。幸运的是,人源化小鼠模型的建立方便了涉及 HCMV 感染的实验室动物实验。单细胞 RNA 测序可在宿主细胞内的单细胞水平上研究病毒与宿主基因表达之间的关系。本研究在单细胞水平上评估了人源化小鼠感染 HCMV 后 PBMC 的基因表达变化,揭示了人源化小鼠感染 HCMV 时病毒与宿主之间的相互作用,为相关研究提供了宝贵的数据集。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Single-cell RNA-sequencing reveals a profound immune cell response in human cytomegalovirus-infected humanized mice
Human cytomegalovirus (HCMV) is a common herpesvirus that persistently infects a large portion of the world's population. Despite the robust host immune response, HCMV is able to replicate, evade host defenses, and establish latency throughout the lifespan by developing multiple immunomodulatory strategies, making the studies on the interaction between HCMV infection and host response particularly important. HCMV has a strict host specificity that specifically infects humans. Therefore, most of the in vivo researches of HCMV rely on clinical samples. Fortunately, the establishment of humanized mouse models allows for convenient in-lab animal experiments involving HCMV infection. Single-cell RNA sequencing enables the study of the relationship between viral and host gene expressions at the single-cell level within host cells. In this study, we assessed the gene expression alterations of PBMCs at the single-cell level within HCMV-infected humanized mice, which sheds light onto the virus-host interactions in the context of HCMV infection of humanized mice and provides a valuable dataset for the related researches.
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来源期刊
Virologica Sinica
Virologica Sinica Biochemistry, Genetics and Molecular Biology-Molecular Medicine
CiteScore
7.70
自引率
1.80%
发文量
3149
期刊介绍: Virologica Sinica is an international journal which aims at presenting the cutting-edge research on viruses all over the world. The journal publishes peer-reviewed original research articles, reviews, and letters to the editor, to encompass the latest developments in all branches of virology, including research on animal, plant and microbe viruses. The journal welcomes articles on virus discovery and characterization, viral epidemiology, viral pathogenesis, virus-host interaction, vaccine development, antiviral agents and therapies, and virus related bio-techniques. Virologica Sinica, the official journal of Chinese Society for Microbiology, will serve as a platform for the communication and exchange of academic information and ideas in an international context. Electronic ISSN: 1995-820X; Print ISSN: 1674-0769
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