远端肢体缺血预处理通过抑制促炎免疫反应和促进脊髓神经元的存活，减轻了脊髓损伤。

IF 2.1 4区医学 Q3 CLINICAL NEUROLOGY

Spinal cord Pub Date : 2024-08-17 DOI:10.1038/s41393-024-01015-3

Yitong Yan, Zihan Li, Shuangyue Zhang, Fan Bai, Yingli Jing, Fubiao Huang, Yan Yu

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引用次数: 0

摘要

研究设计实验动物研究：研究远端肢体缺血预处理（RLPreC）对创伤性脊髓损伤（SCI）的保护作用，并利用RNA测序探讨其潜在的生物学机制：方法：用0.7 N的力诱导小鼠脊髓损伤。进行 RLPreC 治疗。对小鼠的运动功能、疼痛行为和基因表达进行评估：结果：RLPreC治疗可明显改善SCI小鼠的运动功能，减少疼痛行为。RNA-Seq分析确定了5247个差异表达基因（DEGs）。GO 分析显示，这些 DEGs 富含免疫反应、炎症信号转导和突触传递通路。KEGG分析表明，炎症受到抑制，突触相关通路得到促进：结论：RLPreC 是一种很有前景的治疗策略，可改善创伤性 SCI 后的运动功能并减轻疼痛。RNA-Seq分析有助于深入了解潜在的治疗靶点，值得进一步研究。

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Remote limb ischemic preconditioning alleviated spinal cord injury through inhibiting proinflammatory immune response and promoting the survival of spinal neurons

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Remote limb ischemic preconditioning alleviated spinal cord injury through inhibiting proinflammatory immune response and promoting the survival of spinal neurons

Experimental animal study. To investigate the protective effect of remote limb ischemia preconditioning (RLPreC) on traumatic spinal cord injury (SCI) and explore the underlying biological mechanisms using RNA sequencing. China Rehabilitation Science Institute; Beijing; China. spinal cord injury was induced in mice using a force of 0.7 N. RLPreC treatment was administered. Motor function, pain behavior, and gene expression were assessed. RLPreC treatment significantly improved motor function and reduced pain-like behavior in SCI mice. RNA-Seq analysis identified 5247 differentially expressed genes (DEGs). GO analysis revealed enrichment of immune response, inflammatory signaling, and synaptic transmission pathways among these DEGs. KEGG analysis indicated suppression of inflammation and promotion of synapse-related pathways. RLPreC is a promising therapeutic strategy for improving motor function and alleviating pain after traumatic SCI. RNA-Seq analysis provides insights into potential therapeutic targets and warrants further investigation.

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来源期刊

Spinal cord 医学-临床神经学

CiteScore

4.50

自引率

9.10%

发文量

142

审稿时长

2 months

期刊介绍： Spinal Cord is a specialised, international journal that has been publishing spinal cord related manuscripts since 1963. It appears monthly, online and in print, and accepts contributions on spinal cord anatomy, physiology, management of injury and disease, and the quality of life and life circumstances of people with a spinal cord injury. Spinal Cord is multi-disciplinary and publishes contributions across the entire spectrum of research ranging from basic science to applied clinical research. It focuses on high quality original research, systematic reviews and narrative reviews. Spinal Cord''s sister journal Spinal Cord Series and Cases: Clinical Management in Spinal Cord Disorders publishes high quality case reports, small case series, pilot and retrospective studies perspectives, Pulse survey articles, Point-couterpoint articles, correspondences and book reviews. It specialises in material that addresses all aspects of life for persons with spinal cord injuries or disorders. For more information, please see the aims and scope of Spinal Cord Series and Cases.