幽门螺杆菌脂多糖的免疫生物学效应和质子泵抑制剂兰索拉唑对人类多形核白细胞的影响

IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Folia microbiologica Pub Date : 2024-12-01 Epub Date: 2024-08-17 DOI:10.1007/s12223-024-01188-7
Yoji Koshibu, Tsuneyuki Ubagai, Yusuke Yoshino, Yasuo Ono
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引用次数: 0

摘要

幽门螺杆菌定植于人类一半以上的胃黏膜,具有独特的脂多糖(LPS)结构。LPS 是最主要、最适合通过模式识别受体检测的病原体相关分子模式。虽然幽门螺杆菌 LPS 对 PMN 活性氧(ROS)产生的引物效应低于大肠杆菌 O111:B4 LPS,但幽门螺杆菌释放的 LPS 与抗生素根除疗法相关,可能会激活 PMN 并增加 ROS 的产生。此外,我们还描述了幽门螺杆菌和大肠杆菌 O111:B4 LPS 对人类多形核白细胞基因表达和兰索拉唑(LPZ)抗炎作用的影响。从幽门螺杆菌和大肠杆菌O111:B4中分离出的LPS会改变toll样受体2(TLR)和TLR4的表达。然而,来自大肠杆菌 O111:B4 和幽门螺杆菌的 LPS 会使 CD14 的表达分别增加 1.8 倍和 1.5 倍。所有 LPS 亚型都以浓度依赖的方式上调 TNFα 和 IL6 的表达。虽然大肠杆菌 O111:B4 LPS 会上调 IL8R mRNA 水平,但幽门螺杆菌 LPS 不会(≦ 100 ng/mL)。ITGAM 的基因表达水平在使用两种 LPS 时均无明显变化。这些对 PMN 基因表达的不同影响可能取决于 LPS 结构修饰的变化,这与幽门螺杆菌 LPS 的后天免疫调节特性有关。质子泵抑制剂(即 LPZ)与抗生素联合用于根除幽门螺杆菌的治疗。LPZ及其酸激活的磺胺形式AG-2000以剂量依赖的方式抑制PMN产生ROS。这些结果表明,LPZ与抗生素联合用于根除幽门螺杆菌可通过抑制PMN释放ROS来减轻胃部炎症。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Immunobiological effects of lipopolysaccharide derived from Helicobacter pylori and influence of a proton pump inhibitor lansoprazole on human polymorphonuclear leukocytes.

Immunobiological effects of lipopolysaccharide derived from Helicobacter pylori and influence of a proton pump inhibitor lansoprazole on human polymorphonuclear leukocytes.

Helicobacter pylori colonizes the human gastric mucosa of more than half of the human population and has a unique lipopolysaccharide (LPS) structure. LPS is the most dominant and suitable pathogen-associated molecular pattern that is detected via pattern recognition receptors. Although the priming effect of H. pylori LPS on reactive oxygen species (ROS) production of PMNs is lower than that of Escherichia coli O111:B4 LPS, LPS released from H. pylori associated with antibiotics eradication therapy may activate PMNs and increase ROS production. In addition, we describe the effects of H. pylori and E. coli O111:B4 LPSs on gene expression and the anti-inflammatory effect of lansoprazole (LPZ) in human polymorphonuclear leukocytes. LPS isolated from H. pylori and E. coli O111:B4 alters toll-like receptor 2 (TLR) and TLR4 expressions similarly. However, LPS from E. coli O111:B4 and H. pylori caused a 1.8-fold and 1.5-fold increase, respectively, in CD14 expression. All LPS subtypes upregulated TNFα and IL6 expression in a concentration-dependent manner. Although E. coli O111:B4 LPS upregulated IL8R mRNA levels, H. pylori LPS did not (≦ 100 ng/mL). Gene expression levels of ITGAM demonstrated no significant change on using both LPSs. These different effects on the gene expression in PMNs may depend on variations in LPS structural modifications related to the acquired immunomodulatory properties of H. pylori LPS. Proton pump inhibitors, i.e., LPZ, are used in combination with antibiotics for the eradication therapy of H. pylori. LPZ and its acid-activated sulphenamide form AG-2000 suppress ROS production of PMNs in a dose-dependent manner. These results suggest that LPZ combination with antibiotics for H. pylori eradication reduces gastric inflammation by suppressing ROS release from PMNs.

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来源期刊
Folia microbiologica
Folia microbiologica 工程技术-生物工程与应用微生物
CiteScore
5.80
自引率
0.00%
发文量
82
审稿时长
6-12 weeks
期刊介绍: Unlike journals which specialize ever more narrowly, Folia Microbiologica (FM) takes an open approach that spans general, soil, medical and industrial microbiology, plus some branches of immunology. This English-language journal publishes original papers, reviews and mini-reviews, short communications and book reviews. The coverage includes cutting-edge methods and promising new topics, as well as studies using established methods that exhibit promise in practical applications such as medicine, animal husbandry and more. The coverage of FM is expanding beyond Central and Eastern Europe, with a growing proportion of its contents contributed by international authors.
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