揭示最近描述的物种中村芽孢杆菌的次级代谢组和生物防治潜力

IF 6.1 1区 生物学 Q1 MICROBIOLOGY
François Nimbeshaho , Gaspard Nihorimbere , Anthony Argüelles Arias , Charlotte Liénard , Sébastien Steels , Anaclet Nibasumba , Venant Nihorimbere , Anne Legrève , Marc Ongena
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引用次数: 0

摘要

在研究新型潜在生物控制剂的过程中,选取了属于最近描述的中村芽孢杆菌(Bacillus nakamurai)的新菌株 BDI-IS1,因为它对一系列细菌和真菌植物病原体具有很强的体外抗菌活性。通过基因组挖掘和代谢组学研究发现,BDI-IS1 产生多种非核糖体次级代谢产物,包括表面活性素、iturin A、bacillaene、bacillibactin 和 bacilysin,以及一些核糖体合成和翻译后修饰肽(RiPPs),如 plantazolicin,还有可能是 amylocyclicin、bacinapeptin 和 LCI。反向遗传学进一步表明,其中一些化合物在该菌株的拮抗活性中具有特定的参与作用。已测序的五个中村节肢动物菌株之间的比较基因组学研究表明,非核糖体产物构成了该物种的核心代谢组,而RiPPs则更具菌株特异性。虽然次生代谢组缺乏在 B. velezensis 中发现的一些关键生物活性代谢物,但温室实验表明,B. nakamurai BDI-IS1 能够保护番茄和玉米植株免受由 Alternaria solani 和 Exserohilum turcicum 分别引起的早疫病和北方叶枯病的侵害,其保护水平与 B. velezensis QST713 相似或更高。在根部或叶片施用细菌悬浮液后,这些叶面病害都有所减轻,这表明 BDI-IS1 可通过直接抗生素作用和诱导植物防御机制发挥作用。这些研究结果表明,B. nakamurai BDI-IS1 可被视为热带地区植物病害生物防治的理想候选菌种,并鼓励进一步研究其活性范围、要求以及确保其功效所需的条件。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Unravelling the secondary metabolome and biocontrol potential of the recently described species Bacillus nakamurai

In the prospect of novel potential biocontrol agents, a new strain BDI-IS1 belonging to the recently described Bacillus nakamurai was selected for its strong in vitro antimicrobial activities against a range of bacterial and fungal phytopathogens. Genome mining coupled with metabolomics revealed that BDI-IS1 produces multiple non-ribosomal secondary metabolites including surfactin, iturin A, bacillaene, bacillibactin and bacilysin, together with some some ribosomally-synthesized and post-translationally modified peptides (RiPPs) such as plantazolicin, and potentially amylocyclicin, bacinapeptin and LCI. Reverse genetics further showed the specific involvement of some of these compounds in the antagonistic activity of the strain. Comparative genomics between the five already sequenced B. nakamurai strains showed that non-ribosomal products constitute the core metabolome of the species while RiPPs are more strain-specific. Although the secondary metabolome lacks some key bioactive metabolites found in B. velezensis, greenhouse experiments show that B. nakamurai BDI-IS1 is able to protect tomato and maize plants against early blight and northern leaf blight caused by Alternaria solani and Exserohilum turcicum, respectively, at levels similar to or better than B. velezensis QST713. The reduction of these foliar diseases, following root or leaf application of the bacterial suspension demonstrates that BDI-IS1 can act by direct antibiosis and by inducing plant defence mechanisms. These findings indicate that B. nakamurai BDI-IS1 can be considered as a good candidate for biocontrol of plant diseases prevailing in tropical regions, and encourage further research into its spectrum of activity, its requirements and the conditions needed to ensure its efficacy.

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来源期刊
Microbiological research
Microbiological research 生物-微生物学
CiteScore
10.90
自引率
6.00%
发文量
249
审稿时长
29 days
期刊介绍: Microbiological Research is devoted to publishing reports on prokaryotic and eukaryotic microorganisms such as yeasts, fungi, bacteria, archaea, and protozoa. Research on interactions between pathogenic microorganisms and their environment or hosts are also covered.
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