鳜鱼von Hippel-Lindau蛋白通过与IκB相互作用调节NF-κB信号通路,从而促进鱼类Ranavirus的复制。

IF 4 1区 生物学 Q1 ZOOLOGY
Zhi-Min Li, Xiao-Wei Qin, Qi Zhang, Jian He, Min-Cong Liang, Chuan-Rui Li, Yang Yu, Weng-Hui Liu, Shao-Ping Weng, Jian-Guo He, Chang-Jun Guo
{"title":"鳜鱼von Hippel-Lindau蛋白通过与IκB相互作用调节NF-κB信号通路,从而促进鱼类Ranavirus的复制。","authors":"Zhi-Min Li, Xiao-Wei Qin, Qi Zhang, Jian He, Min-Cong Liang, Chuan-Rui Li, Yang Yu, Weng-Hui Liu, Shao-Ping Weng, Jian-Guo He, Chang-Jun Guo","doi":"10.24272/j.issn.2095-8137.2023.392","DOIUrl":null,"url":null,"abstract":"<p><p>The von Hippel-Lindau tumor suppressor protein (VHL), an E3 ubiquitin ligase, functions as a critical regulator of the oxygen-sensing pathway for targeting hypoxia-inducible factors. Recent evidence suggests that mammalian VHL may also be critical to the NF-κB signaling pathway, although the specific molecular mechanisms remain unclear. Herein, the roles of mandarin fish ( <i>Siniperca chuatsi</i>) VHL ( <i>sc</i>VHL) in the NF-κB signaling pathway and mandarin fish ranavirus (MRV) replication were explored. The transcription of <i>sc</i>VHL was induced by immune stimulation and MRV infection, indicating a potential role in innate immunity. Dual-luciferase reporter gene assays and reverse transcription quantitative PCR (RT-qPCR) results demonstrated that <i>sc</i>VHL evoked and positively regulated the NF-κB signaling pathway. Treatment with NF-κB signaling pathway inhibitors indicated that the role of <i>sc</i>VHL may be mediated through <i>sc</i>IKKα, <i>sc</i>IKKβ, <i>sc</i>IκBα, or <i>sc</i>p65. Co-immunoprecipitation (Co-IP) analysis identified <i>sc</i>IκBα as a novel target protein of <i>sc</i>VHL. Moreover, <i>sc</i>VHL targeted <i>sc</i>IκBα to catalyze the formation of K63-linked polyubiquitin chains to activate the NF-κB signaling pathway. Following MRV infection, NF-κB signaling remained activated, which, in turn, promoted MRV replication. These findings suggest that <i>sc</i>VHL not only positively regulates NF-κB but also significantly enhances MRV replication. This study reveals a novel function of <i>sc</i>VHL in NF-κB signaling and viral infection in fish.</p>","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"45 5","pages":"990-1000"},"PeriodicalIF":4.0000,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11491782/pdf/","citationCount":"0","resultStr":"{\"title\":\"Mandarin fish von Hippel-Lindau protein regulates the NF-κB signaling pathway via interaction with IκB to promote fish ranavirus replication.\",\"authors\":\"Zhi-Min Li, Xiao-Wei Qin, Qi Zhang, Jian He, Min-Cong Liang, Chuan-Rui Li, Yang Yu, Weng-Hui Liu, Shao-Ping Weng, Jian-Guo He, Chang-Jun Guo\",\"doi\":\"10.24272/j.issn.2095-8137.2023.392\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The von Hippel-Lindau tumor suppressor protein (VHL), an E3 ubiquitin ligase, functions as a critical regulator of the oxygen-sensing pathway for targeting hypoxia-inducible factors. Recent evidence suggests that mammalian VHL may also be critical to the NF-κB signaling pathway, although the specific molecular mechanisms remain unclear. Herein, the roles of mandarin fish ( <i>Siniperca chuatsi</i>) VHL ( <i>sc</i>VHL) in the NF-κB signaling pathway and mandarin fish ranavirus (MRV) replication were explored. The transcription of <i>sc</i>VHL was induced by immune stimulation and MRV infection, indicating a potential role in innate immunity. Dual-luciferase reporter gene assays and reverse transcription quantitative PCR (RT-qPCR) results demonstrated that <i>sc</i>VHL evoked and positively regulated the NF-κB signaling pathway. Treatment with NF-κB signaling pathway inhibitors indicated that the role of <i>sc</i>VHL may be mediated through <i>sc</i>IKKα, <i>sc</i>IKKβ, <i>sc</i>IκBα, or <i>sc</i>p65. Co-immunoprecipitation (Co-IP) analysis identified <i>sc</i>IκBα as a novel target protein of <i>sc</i>VHL. Moreover, <i>sc</i>VHL targeted <i>sc</i>IκBα to catalyze the formation of K63-linked polyubiquitin chains to activate the NF-κB signaling pathway. Following MRV infection, NF-κB signaling remained activated, which, in turn, promoted MRV replication. These findings suggest that <i>sc</i>VHL not only positively regulates NF-κB but also significantly enhances MRV replication. This study reveals a novel function of <i>sc</i>VHL in NF-κB signaling and viral infection in fish.</p>\",\"PeriodicalId\":48636,\"journal\":{\"name\":\"Zoological Research\",\"volume\":\"45 5\",\"pages\":\"990-1000\"},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2024-09-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11491782/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zoological Research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.24272/j.issn.2095-8137.2023.392\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ZOOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zoological Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.24272/j.issn.2095-8137.2023.392","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ZOOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

Von Hippel-Lindau肿瘤抑制蛋白(VHL)是一种E3泛素连接酶,是氧传感通路的关键调节因子,可靶向缺氧诱导因子。最近的证据表明,哺乳动物的 VHL 也可能是 NF-κB 信号通路的关键,但具体的分子机制仍不清楚。本文探讨了鳜鱼VHL(scVHL)在NF-κB信号通路和鳜鱼病毒(MRV)复制中的作用。scVHL的转录受免疫刺激和MRV感染的诱导,表明其在先天性免疫中的潜在作用。双荧光素酶报告基因检测和反转录定量 PCR(RT-qPCR)结果表明,scVHL 可诱导并正向调节 NF-κB 信号通路。用NF-κB信号通路抑制剂处理表明,scVHL的作用可能是通过scIKKα、scIKKβ、scIκBα或scp65介导的。共免疫沉淀(Co-IP)分析发现,scIκBα是scVHL的一种新型靶蛋白。此外,scVHL以scIκBα为靶蛋白,催化K63连接的多泛素链的形成,从而激活NF-κB信号通路。MRV感染后,NF-κB信号仍处于激活状态,这反过来又促进了MRV的复制。这些发现表明,scVHL 不仅能正向调节 NF-κB,还能显著增强 MRV 复制。这项研究揭示了scVHL在鱼类NF-κB信号传导和病毒感染中的新功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mandarin fish von Hippel-Lindau protein regulates the NF-κB signaling pathway via interaction with IκB to promote fish ranavirus replication.

The von Hippel-Lindau tumor suppressor protein (VHL), an E3 ubiquitin ligase, functions as a critical regulator of the oxygen-sensing pathway for targeting hypoxia-inducible factors. Recent evidence suggests that mammalian VHL may also be critical to the NF-κB signaling pathway, although the specific molecular mechanisms remain unclear. Herein, the roles of mandarin fish ( Siniperca chuatsi) VHL ( scVHL) in the NF-κB signaling pathway and mandarin fish ranavirus (MRV) replication were explored. The transcription of scVHL was induced by immune stimulation and MRV infection, indicating a potential role in innate immunity. Dual-luciferase reporter gene assays and reverse transcription quantitative PCR (RT-qPCR) results demonstrated that scVHL evoked and positively regulated the NF-κB signaling pathway. Treatment with NF-κB signaling pathway inhibitors indicated that the role of scVHL may be mediated through scIKKα, scIKKβ, scIκBα, or scp65. Co-immunoprecipitation (Co-IP) analysis identified scIκBα as a novel target protein of scVHL. Moreover, scVHL targeted scIκBα to catalyze the formation of K63-linked polyubiquitin chains to activate the NF-κB signaling pathway. Following MRV infection, NF-κB signaling remained activated, which, in turn, promoted MRV replication. These findings suggest that scVHL not only positively regulates NF-κB but also significantly enhances MRV replication. This study reveals a novel function of scVHL in NF-κB signaling and viral infection in fish.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Zoological Research
Zoological Research Medicine-General Medicine
CiteScore
7.60
自引率
10.20%
发文量
1937
审稿时长
8 weeks
期刊介绍: Established in 1980, Zoological Research (ZR) is a bimonthly publication produced by Kunming Institute of Zoology, the Chinese Academy of Sciences, and the China Zoological Society. It publishes peer-reviewed original research article/review/report/note/letter to the editor/editorial in English on Primates and Animal Models, Conservation and Utilization of Animal Resources, and Animal Diversity and Evolution.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信