Ling Wang, Wei-Qiang Liu, Juan Du, Meng Li, Rui-Feng Wu, Ming Li
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Comparative genomic methylation analysis demonstrated that genes associated with differentially methylated regions were involved in membrane fusion, vesicular formation and trafficking, hemoglobin function, cell cycle regulation, and neuronal differentiation. These results suggest that the high-altitude-related epigenetic modifications are extensive, involving a complete adaptation process from the inhibition of single Ca <sup>2+</sup> channel proteins to multiple proteins collaboratively enhancing vesicular function or inhibiting cell differentiation and proliferation. Functional assays demonstrated that overexpression or down-regulation of candidate genes, such as <i>SNX10</i>, <i>TIMELESS</i>, and <i>CACYBP</i>, influenced cell viability under stress conditions. Overall, this research suggests that comparing DNA methylation across closely related species can identify novel candidate genomic regions and genes associated with local adaptations, thereby deepening our understanding of the mechanisms underlying environmental adaptations.</p>","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":null,"pages":null},"PeriodicalIF":4.0000,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11491775/pdf/","citationCount":"0","resultStr":"{\"title\":\"Comparative DNA methylation reveals epigenetic adaptation to high altitude in snub-nosed monkeys.\",\"authors\":\"Ling Wang, Wei-Qiang Liu, Juan Du, Meng Li, Rui-Feng Wu, Ming Li\",\"doi\":\"10.24272/j.issn.2095-8137.2024.050\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>DNA methylation plays a crucial role in environmental adaptations. 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引用次数: 0
摘要
DNA甲基化在环境适应中起着至关重要的作用。在这里,我们利用全基因组亚硫酸氢盐测序技术,对高海拔的滇金丝猴和近缘的金丝猴进行了全面的全基因组DNA甲基化分析。我们的研究结果表明,与滇金丝猴相比,金丝猴的整体DNA甲基化水平略有上升,这表明前者基因组高甲基化区域的发生率更高。基因组甲基化比较分析表明,与不同甲基化区域相关的基因涉及膜融合、囊泡形成和贩运、血红蛋白功能、细胞周期调控和神经元分化。这些结果表明,与高海拔有关的表观遗传修饰是广泛的,涉及一个完整的适应过程,从抑制单个 Ca 2+ 通道蛋白到多个蛋白协同增强囊泡功能或抑制细胞分化和增殖。功能测试表明,SNX10、TIMELESS 和 CACYBP 等候选基因的过表达或下调会影响应激条件下的细胞活力。总之,这项研究表明,比较近缘物种之间的DNA甲基化可以发现与局部适应相关的新候选基因组区域和基因,从而加深我们对环境适应机制的理解。
Comparative DNA methylation reveals epigenetic adaptation to high altitude in snub-nosed monkeys.
DNA methylation plays a crucial role in environmental adaptations. Here, using whole-genome bisulfite sequencing, we generated comprehensive genome-wide DNA methylation profiles for the high-altitude Yunnan snub-nosed monkey ( Rhinopithecus bieti) and the closely related golden snub-nosed monkey ( R. roxellana). Our findings indicated a slight increase in overall DNA methylation levels in golden snub-nosed monkeys compared to Yunnan snub-nosed monkeys, suggesting a higher prevalence of hypermethylated genomic regions in the former. Comparative genomic methylation analysis demonstrated that genes associated with differentially methylated regions were involved in membrane fusion, vesicular formation and trafficking, hemoglobin function, cell cycle regulation, and neuronal differentiation. These results suggest that the high-altitude-related epigenetic modifications are extensive, involving a complete adaptation process from the inhibition of single Ca 2+ channel proteins to multiple proteins collaboratively enhancing vesicular function or inhibiting cell differentiation and proliferation. Functional assays demonstrated that overexpression or down-regulation of candidate genes, such as SNX10, TIMELESS, and CACYBP, influenced cell viability under stress conditions. Overall, this research suggests that comparing DNA methylation across closely related species can identify novel candidate genomic regions and genes associated with local adaptations, thereby deepening our understanding of the mechanisms underlying environmental adaptations.
期刊介绍:
Established in 1980, Zoological Research (ZR) is a bimonthly publication produced by Kunming Institute of Zoology, the Chinese Academy of Sciences, and the China Zoological Society. It publishes peer-reviewed original research article/review/report/note/letter to the editor/editorial in English on Primates and Animal Models, Conservation and Utilization of Animal Resources, and Animal Diversity and Evolution.