{"title":"通过伽马射线照射打破 Triticum aestivum-Aegilops speltoides 衍生物中杀配子基因与叶锈病抗性基因（LrS2427）之间的关联。","authors":"R Ragini, Niranjana Murukan, Navpreet Kaur Sekhon, Chetna Chugh, Priyanka Agarwal, Prachi Yadav, Niharika Mallick, Shailendra Kumar Jha, Mir Asif Iquebal, Gitanjali Tandon, Aakriti Verma, Bhupinder Singh, Sherry Rachel Jacob, K Raghunandan, Kumble Vinod Prabhu, Shivmangal Singh Tomar, Vinod","doi":"10.1007/s11032-024-01491-8","DOIUrl":null,"url":null,"abstract":"<p><p>Utilization of crop wild relatives of wheat can be very effective in building the genetic diversity to cater to the evolving strains of disease pathogens. <i>Aegilops speltoides</i> is a rich source of rust resistance genes however transferring those to wheat genome can be tedious due to co-transfer and preferential transmission of undesirable genes causing gametocidal activity. Such an unholy association was observed in <i>Triticum aestivum</i>-<i>Ae. speltoides</i> derivative line Sel. 2427 which possess the broad-spectrum leaf rust seedling resistance gene (<i>LrS2427</i>). Molecular analysis based on 35 K wheat breeder's array revealed the maximum percentage of <i>Ae. speltoides</i> genome introgression on homoeologous group 2. In situ hybridization studies revealed the presence of S genome in Sel. 2427, showing six translocations on four chromosomes. Karyotyping using repetitive probe (AAG)<sub>6</sub> revealed that the two chromosomes involved are 2D and 2B. Genic regions causing gametocidal activity were identified by dissecting it into component traits and QTLs on 2D and 2B chromosomes were revealed in case of the trait seed shrivelling index. To break the inadvertent association of <i>LrS2427</i> with gametocidal genes, F<sub>1</sub>(Agra Local X Sel. 2427) seeds were irradiated with gamma rays and stable leaf rust resistant mutants lacking gametocidal activity were developed. These mutants showed resistance to different races of leaf rust pathogen and showed superior agronomic performance as well. These mutants could be a great resource in wheat improvement for utilization of the leaf rust resistance gene <i>LrS2427</i> without any yield penalty.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01491-8.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 8","pages":"54"},"PeriodicalIF":2.6000,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11322474/pdf/","citationCount":"0","resultStr":"{\"title\":\"Breaking the association between gametocidal gene(s) and leaf rust resistance gene (<i>LrS2427</i>) in <i>Triticum aestivum</i>-<i>Aegilops speltoides</i> derivative by gamma irradiation.\",\"authors\":\"R Ragini, Niranjana Murukan, Navpreet Kaur Sekhon, Chetna Chugh, Priyanka Agarwal, Prachi Yadav, Niharika Mallick, Shailendra Kumar Jha, Mir Asif Iquebal, Gitanjali Tandon, Aakriti Verma, Bhupinder Singh, Sherry Rachel Jacob, K Raghunandan, Kumble Vinod Prabhu, Shivmangal Singh Tomar, Vinod\",\"doi\":\"10.1007/s11032-024-01491-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Utilization of crop wild relatives of wheat can be very effective in building the genetic diversity to cater to the evolving strains of disease pathogens. <i>Aegilops speltoides</i> is a rich source of rust resistance genes however transferring those to wheat genome can be tedious due to co-transfer and preferential transmission of undesirable genes causing gametocidal activity. Such an unholy association was observed in <i>Triticum aestivum</i>-<i>Ae. speltoides</i> derivative line Sel. 2427 which possess the broad-spectrum leaf rust seedling resistance gene (<i>LrS2427</i>). Molecular analysis based on 35 K wheat breeder's array revealed the maximum percentage of <i>Ae. speltoides</i> genome introgression on homoeologous group 2. In situ hybridization studies revealed the presence of S genome in Sel. 2427, showing six translocations on four chromosomes. Karyotyping using repetitive probe (AAG)<sub>6</sub> revealed that the two chromosomes involved are 2D and 2B. Genic regions causing gametocidal activity were identified by dissecting it into component traits and QTLs on 2D and 2B chromosomes were revealed in case of the trait seed shrivelling index. To break the inadvertent association of <i>LrS2427</i> with gametocidal genes, F<sub>1</sub>(Agra Local X Sel. 2427) seeds were irradiated with gamma rays and stable leaf rust resistant mutants lacking gametocidal activity were developed. These mutants showed resistance to different races of leaf rust pathogen and showed superior agronomic performance as well. These mutants could be a great resource in wheat improvement for utilization of the leaf rust resistance gene <i>LrS2427</i> without any yield penalty.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01491-8.</p>\",\"PeriodicalId\":18769,\"journal\":{\"name\":\"Molecular Breeding\",\"volume\":\"44 8\",\"pages\":\"54\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-08-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11322474/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular Breeding\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s11032-024-01491-8\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/8/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Breeding","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11032-024-01491-8","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/8/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
Breaking the association between gametocidal gene(s) and leaf rust resistance gene (LrS2427) in Triticum aestivum-Aegilops speltoides derivative by gamma irradiation.
Utilization of crop wild relatives of wheat can be very effective in building the genetic diversity to cater to the evolving strains of disease pathogens. Aegilops speltoides is a rich source of rust resistance genes however transferring those to wheat genome can be tedious due to co-transfer and preferential transmission of undesirable genes causing gametocidal activity. Such an unholy association was observed in Triticum aestivum-Ae. speltoides derivative line Sel. 2427 which possess the broad-spectrum leaf rust seedling resistance gene (LrS2427). Molecular analysis based on 35 K wheat breeder's array revealed the maximum percentage of Ae. speltoides genome introgression on homoeologous group 2. In situ hybridization studies revealed the presence of S genome in Sel. 2427, showing six translocations on four chromosomes. Karyotyping using repetitive probe (AAG)6 revealed that the two chromosomes involved are 2D and 2B. Genic regions causing gametocidal activity were identified by dissecting it into component traits and QTLs on 2D and 2B chromosomes were revealed in case of the trait seed shrivelling index. To break the inadvertent association of LrS2427 with gametocidal genes, F1(Agra Local X Sel. 2427) seeds were irradiated with gamma rays and stable leaf rust resistant mutants lacking gametocidal activity were developed. These mutants showed resistance to different races of leaf rust pathogen and showed superior agronomic performance as well. These mutants could be a great resource in wheat improvement for utilization of the leaf rust resistance gene LrS2427 without any yield penalty.
Supplementary information: The online version contains supplementary material available at 10.1007/s11032-024-01491-8.
期刊介绍：
Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer.
All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others.
Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards.
Molecular Breeding core areas:
Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.
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