用海藻酸盐对牙本质粉进行表面改性,并评估其对牙髓干细胞的活力和增殖(体外)及其生物相容性(体内)的影响。

IF 3.5 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Melika Manzarpour DDS , Mohammad Reza Mousavi DDS , Yas Mahdavinaderi DDS , Mohammadali Najimi DDS , Amin Ghalambor DDS , Sadegh Hasannia PhD , Sarah Rajabi PhD , Mohamad Pezeshki-Modaress PhD , Amir Kamali PhD , Hengameh Bakhtiar DDS, MSc, FRCD
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引用次数: 0

摘要

简介:本研究旨在合成海藻酸盐表面修饰的牙本质粉末(一种潜在的牙髓再生物质),并评估其在体外对人牙髓干细胞(hDPSCs)的活力和增殖的影响及其在体内的生物相容性:在体外阶段,将牙本质粉末合成为三个尺寸组(150-250 μm、250-500 μm、500-1000 μm),然后进行脱矿和羚肽化处理,以去除牙本质胶原端肽并消除宿主免疫反应。用海藻酸盐进行表面改性后,用场发射扫描电子显微镜(FE-SEM)、能量色散 X 射线光谱(EDX)和细胞活力与增殖测试对 hDPSCs 进行了 14 天的研究。在体内阶段,将牙本质粉植入大鼠腓骨缺损处 8 周,并进行组织学分析。所有非参数数据均采用 Kruskal- Wallis 检验进行分析,所有定量数据均采用 SPSS 和 PResults 进行单因素方差分析:各组脱钙和脱肽均成功。各组细胞存活率最佳且相同(P>0.05)。500-1000 μm 组的细胞增殖率明显更高(PC结论:去矿物质和阿特肽的表面改性在所有组中都是成功的:用海藻酸盐对脱矿和阿特肽化的牙本质粉进行表面修饰,可增强表面物理性质和细胞增殖,同时在组织内显示出良好的生物相容性,并降低宿主免疫反应。这些发现为牙本质-牙髓复合体的再生带来了希望。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Surface Modification of Dentin Powder With Alginate and Evaluation of Its Effects on the Viability and Proliferation of Dental Pulp Stem Cells (In Vitro), Its Biocompatibility (In Vivo)

Introduction

This study aimed to synthesize dentin powder surface modified with alginate, a potential substance for dental pulp regeneration, and evaluate its effects on the viability and proliferation of human dental pulp stem cells in vitro and its biocompatibility in vivo.

Methods

In the in vitro phase, dentin powder was synthesized in 3 size groups (150–250 μm, 250–500 μm, and 500–1000 μm) after demineralization and atelopeptidization which is used to remove dentin collagen telopeptides and eliminate host immune response. Surface modification with alginate was performed and followed by field-emission scanning electron microscopy, energy dispersive X-ray spectroscopy, and cell viability and proliferation testing for 14 days with human dental pulp stem cells studied. In the in vivo phase, dentin powders were implanted in rat calvarial defects for 8 weeks, and histologic analysis was conducted. All nonparametric data were analyzed with the Kruskal–Wallis test, and all the quantitative data were analyzed by 1-way analysis of variance using SPSS, and P < .05 was considered statistically significant.

Results

Demineralization and atelopeptidization were successful in all groups. Cell viability was optimal and equal (P > .05) in all groups. The 500- to 1000-μm group exhibited significantly higher cell proliferation (P < .05). Histologic assessment shows acceptable biocompatibility in all groups; the angiogenesis score was significantly greater in both 250–500 and 500–1000, and minimal inflammatory response was noted in the 500- to 1000-μm group, and the amount of newly formed bone in this group was higher than other groups.

Conclusions

Surface modification of demineralized and atelopeptidized dentin powder with alginate enhanced surface physical properties and cell proliferation while showing great biocompatibility within tissue and reducing the host immune response. These findings hold promise for dentin-pulp complex regeneration.
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来源期刊
Journal of endodontics
Journal of endodontics 医学-牙科与口腔外科
CiteScore
8.80
自引率
9.50%
发文量
224
审稿时长
42 days
期刊介绍: The Journal of Endodontics, the official journal of the American Association of Endodontists, publishes scientific articles, case reports and comparison studies evaluating materials and methods of pulp conservation and endodontic treatment. Endodontists and general dentists can learn about new concepts in root canal treatment and the latest advances in techniques and instrumentation in the one journal that helps them keep pace with rapid changes in this field.
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