Harkirat Singh, M. Padmaja, Aparna M. Thomas, Harsh Panwar, Shaikh I. Nasrul, Jyoti, Nirbhay K. Singh
{"title":"通过多重聚合酶链式反应对印度旁遮普邦犬蜱血寄生虫的分子调查。","authors":"Harkirat Singh, M. Padmaja, Aparna M. Thomas, Harsh Panwar, Shaikh I. Nasrul, Jyoti, Nirbhay K. Singh","doi":"10.1007/s11686-024-00856-5","DOIUrl":null,"url":null,"abstract":"<div><h3>Purpose</h3><p>Tick-transmitted parasites as <i>Babesia gibsoni</i>, <i>Babesia vogeli</i>, <i>Ehrlichia canis</i>, and <i>Hepatozoon canis</i> are major health concern for dogs. Owing to prevalence and infection severity, there is need of sensitive, specific, and affordable test for their simultaneous detection.</p><h3>Methods</h3><p>Prevalence of <i>B. gibsoni</i>, <i>B. vogeli</i>, <i>E. canis</i>, and <i>H. canis</i> infections was assessed on 719 blood samples by microscopy and multiplex PCR assay targeting <i>18S rRNA</i> (<i>B. gibsoni</i> & <i>H. canis</i>), <i>ITS1 & 5.8S rRNA</i> (<i>B. vogeli</i>) and <i>VirB9</i> gene (<i>E. canis</i>). An internal control (canine-<i>actin</i>) was also included to increase the accuracy of assay and effect of associated risk factors with disease prevalence was also studied.</p><h3>Results</h3><p>Microscopic prevalence of <i>B. gibsoni</i>, <i>B. vogeli, E. canis</i> and <i>H. canis</i> was 5.0%, 0.1%, 1.4% and 1.0%, respectively, whereas with multiplex PCR assay, the corresponding values were 8.9%, 1.1%, 2.6% and 5.1% besides concurrent infections of <i>B. gibsoni</i> & <i>H. canis</i> (0.4%), <i>B. gibsoni</i> & <i>E. canis</i> (0.4%), <i>E. canis</i> & <i>H. canis</i> (0.3%) and <i>B. gibsoni</i> & <i>B. vogeli</i> (0.1%). Analytical sensitivity of developed assay was 0.1pg (<i>B. gibsoni</i> & <i>H. canis</i>), 0.01pg (<i>B. vogeli</i>), and 1.0pg (<i>E. canis</i>). A ″fair″ (<i>B. vogeli</i> & <i>H. canis</i>) to ″substantial″ (<i>B. gibsoni</i> & <i>E. canis</i>) agreement between two tests was observed with data as statistically significant. Breed, sex and location were significantly associated with <i>B. gibsoni</i> infection.</p><h3>Conclusion</h3><p>The developed multiplex PCR assay offers a potential solution to detect these pathogens simultaneously, aiding in timely diagnosis and effective disease management in suspected dogs.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 3","pages":"1458 - 1470"},"PeriodicalIF":1.2000,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11686-024-00856-5.pdf","citationCount":"0","resultStr":"{\"title\":\"Molecular Survey of Tick-Borne Haemoparasites of Dogs by Multiplex Polymerase Chain Reaction from Punjab, India\",\"authors\":\"Harkirat Singh, M. Padmaja, Aparna M. Thomas, Harsh Panwar, Shaikh I. Nasrul, Jyoti, Nirbhay K. Singh\",\"doi\":\"10.1007/s11686-024-00856-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Purpose</h3><p>Tick-transmitted parasites as <i>Babesia gibsoni</i>, <i>Babesia vogeli</i>, <i>Ehrlichia canis</i>, and <i>Hepatozoon canis</i> are major health concern for dogs. Owing to prevalence and infection severity, there is need of sensitive, specific, and affordable test for their simultaneous detection.</p><h3>Methods</h3><p>Prevalence of <i>B. gibsoni</i>, <i>B. vogeli</i>, <i>E. canis</i>, and <i>H. canis</i> infections was assessed on 719 blood samples by microscopy and multiplex PCR assay targeting <i>18S rRNA</i> (<i>B. gibsoni</i> & <i>H. canis</i>), <i>ITS1 & 5.8S rRNA</i> (<i>B. vogeli</i>) and <i>VirB9</i> gene (<i>E. canis</i>). An internal control (canine-<i>actin</i>) was also included to increase the accuracy of assay and effect of associated risk factors with disease prevalence was also studied.</p><h3>Results</h3><p>Microscopic prevalence of <i>B. gibsoni</i>, <i>B. vogeli, E. canis</i> and <i>H. canis</i> was 5.0%, 0.1%, 1.4% and 1.0%, respectively, whereas with multiplex PCR assay, the corresponding values were 8.9%, 1.1%, 2.6% and 5.1% besides concurrent infections of <i>B. gibsoni</i> & <i>H. canis</i> (0.4%), <i>B. gibsoni</i> & <i>E. canis</i> (0.4%), <i>E. canis</i> & <i>H. canis</i> (0.3%) and <i>B. gibsoni</i> & <i>B. vogeli</i> (0.1%). Analytical sensitivity of developed assay was 0.1pg (<i>B. gibsoni</i> & <i>H. canis</i>), 0.01pg (<i>B. vogeli</i>), and 1.0pg (<i>E. canis</i>). A ″fair″ (<i>B. vogeli</i> & <i>H. canis</i>) to ″substantial″ (<i>B. gibsoni</i> & <i>E. canis</i>) agreement between two tests was observed with data as statistically significant. Breed, sex and location were significantly associated with <i>B. gibsoni</i> infection.</p><h3>Conclusion</h3><p>The developed multiplex PCR assay offers a potential solution to detect these pathogens simultaneously, aiding in timely diagnosis and effective disease management in suspected dogs.</p></div>\",\"PeriodicalId\":6932,\"journal\":{\"name\":\"Acta Parasitologica\",\"volume\":\"69 3\",\"pages\":\"1458 - 1470\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2024-08-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://link.springer.com/content/pdf/10.1007/s11686-024-00856-5.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Parasitologica\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s11686-024-00856-5\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Parasitologica","FirstCategoryId":"3","ListUrlMain":"https://link.springer.com/article/10.1007/s11686-024-00856-5","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Molecular Survey of Tick-Borne Haemoparasites of Dogs by Multiplex Polymerase Chain Reaction from Punjab, India
Purpose
Tick-transmitted parasites as Babesia gibsoni, Babesia vogeli, Ehrlichia canis, and Hepatozoon canis are major health concern for dogs. Owing to prevalence and infection severity, there is need of sensitive, specific, and affordable test for their simultaneous detection.
Methods
Prevalence of B. gibsoni, B. vogeli, E. canis, and H. canis infections was assessed on 719 blood samples by microscopy and multiplex PCR assay targeting 18S rRNA (B. gibsoni & H. canis), ITS1 & 5.8S rRNA (B. vogeli) and VirB9 gene (E. canis). An internal control (canine-actin) was also included to increase the accuracy of assay and effect of associated risk factors with disease prevalence was also studied.
Results
Microscopic prevalence of B. gibsoni, B. vogeli, E. canis and H. canis was 5.0%, 0.1%, 1.4% and 1.0%, respectively, whereas with multiplex PCR assay, the corresponding values were 8.9%, 1.1%, 2.6% and 5.1% besides concurrent infections of B. gibsoni & H. canis (0.4%), B. gibsoni & E. canis (0.4%), E. canis & H. canis (0.3%) and B. gibsoni & B. vogeli (0.1%). Analytical sensitivity of developed assay was 0.1pg (B. gibsoni & H. canis), 0.01pg (B. vogeli), and 1.0pg (E. canis). A ″fair″ (B. vogeli & H. canis) to ″substantial″ (B. gibsoni & E. canis) agreement between two tests was observed with data as statistically significant. Breed, sex and location were significantly associated with B. gibsoni infection.
Conclusion
The developed multiplex PCR assay offers a potential solution to detect these pathogens simultaneously, aiding in timely diagnosis and effective disease management in suspected dogs.
期刊介绍：
Acta Parasitologica is an international journal covering the latest advances in the subject.
Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews.
The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
