比较评估 G6PD 缺乏的供体和健康供体制备的红细胞浓缩物（RCC）在 RCC 储存期间的氧化和生化参数。

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-08-13 DOI:10.1016/j.tracli.2024.08.003

Samira Moshkelgosha , Mohammad Reza Deyhim , Ramazan Ali Khavari-Nejad , Mahdieh Meschi

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引用次数: 0

摘要

简介葡萄糖-6-磷酸脱氢酶（G6PD）缺乏症是红细胞（RBC）中最常见的遗传性酶紊乱。鉴于 G6PD 酶在红细胞中作为抗氧化剂的重要性，我们试图研究 G6PD 酶缺乏症供体与健康供体制备的红细胞浓缩物（RCC）中的氧化损伤：这项横断面研究的对象是 20 名男性供体。材料方法：这项横断面研究以 20 名男性供体为对象，其中 10 人患有 G6PD 缺乏症（作为病例），其他人酶活性正常（作为对照）。在 RCC 储存的第 0 天、第 7 天、第 14 天、第 21 天、第 28 天和第 35 天，对两组供体制备的 RCC 的生化指标和氧化损伤指标进行检测；数据比较采用 SPSS 统计软件进行分析：结果显示，与对照组（PC）相比，G6PD 缺乏供体的乳酸浓度在 RCC 储存第 7 天至第 35 天显著增加：我们的研究表明，与健康供体相比，G6PD缺陷供体的氧化变化明显增加，这可能会导致RCC储存病变和输血并发症的增加。鉴于 G6PD 酶缺乏症在大流行地区的高发病率，似乎应将酶筛查纳入献血者筛查计划。

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Comparative evaluation of oxidative and biochemical parameters of red cell concentrates (RCCs) prepared from G6PD deficient donors and healthy donors during RCC storage

Introduction

Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common inherited enzyme disorder in red blood cell (RBC). Due to the importance of G6PD enzyme as an antioxidant in RBC, we tried to investigate the oxidative damage in red cell concentrates (RCCs) prepared from donors with G6PD enzyme deficiency in comparison with healthy donors.

Material method

This cross-sectional study was conducted on 20 male donors. Ten of the donors had G6PD deficiency (as a case) and the others had normal enzyme activity (as a control). Biochemical and oxidative damage parameters were examined in RCCs prepared from two groups on days 0, 7, 14, 21, 28 and 35 of RCCs storage; data comparison was analyzed by SPSS statistical software.

Results

According to the result, lactate concentration increased significantly from the 7th day to the 35th day of RCC storage in G6PD-deficient donors compared to the control (P < 0.05). In addition, malondialdehyde (MDA) concentration in G6PD-deficient RCC showed a significant increase compared to the control in all days of storage (P < 0.05). Among the hematological parameters, mean corpuscular volume (MCV) and mean cell hemoglobin (MCH) increased significantly in all days of RCC storage in G6PD-deficient donors compared to the control (P < 0.05).

Conclusion

Our study showed that oxidative changes in G6PD-deficient donors were significantly increased compared to the healthy donors, which probably leads to RCC storage lesion and an increase in blood transfusion complications. Due to the high prevalence of G6PD enzyme deficiency in pandemic areas, it seems that enzyme screening should be included in donor screening programs.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.