MAPK3-MYB36-ARF1 模块调控丹参中丹参酮的形成。

Plant signaling & behavior Pub Date : 2024-12-31 Epub Date: 2024-08-15 DOI:10.1080/15592324.2024.2391659
Yongfeng Xie, Hao Liu
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引用次数: 0

摘要

丹参,又名丹参,是一种传统的中药植物,对心血管有显著疗效,这归功于它的次生代谢产物,尤其是丹参酮。尽管丹参酮具有药用价值,但其天然含量较低,因此有必要进行研究以提高其含量。本研究探讨了 ARF 转录因子(SmARF1)在丹参酮积累中的作用。在毛根中过表达 SmARF1 能显著提高丹参酮的含量。EMSA 和 Dual-LUC 分析表明,与 SmMAPK3 相互作用的转录因子 SmMYB36 与 SmARF1 启动子结合并对其进行调控。单独使用 SmMYB36 可抑制 SmARF1 基因的表达,而与 SmMAPK3 相互作用则可增强 SmARF1 启动子的活性。这一 MAPK3-MYB36-ARF1 模块阐明了丹参酮生物合成的复杂调控机制,为通过先进的生物技术方法有针对性地提高丹参酮含量提供了启示。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
MAPK3-MYB36-ARF1 module regulates the tanshinone formation in Salvia miltiorrhiza.

Salvia miltiorrhiza, known as Danshen, is a traditional Chinese medicinal plant with significant cardiovascular benefits, attributed to its secondary metabolites, particularly tanshinones. Despite their medicinal value, tanshinones occur in low natural abundance, necessitating research to increase their content. This study explores the role of the ARF transcription factor (SmARF1) in tanshinone accumulation in Danshen. Overexpressing SmARF1 in hairy roots significantly increased tanshinone levels. EMSA and Dual-LUC assays revealed that SmMYB36, a transcription factor interacting with SmMAPK3, binds to and regulates the SmARF1 promoter. SmMYB36 alone inhibited the expression of SmARF1 gene, while its interaction with SmMAPK3 enhanced SmARF1 promoter activity. This MAPK3-MYB36-ARF1 module elucidates a complex regulatory mechanism for tanshinone biosynthesis, offering insights for targeted enhancement of tanshinone content through advanced biotechnological approaches.

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