TRIM5 通过促进 RIG-I 介导的 I 型干扰素抗病毒反应来抑制塞内卡病毒 A 的复制。

IF 3.7 1区 农林科学 Q1 VETERINARY SCIENCES
Huizi Li, Zhenxin Xie, Xiaoling Lei, Ming Chen, Tingting Zheng, Cunhao Lin, Zhangyong Ning
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引用次数: 0

摘要

塞内卡病毒 A(SVA)是一种新出现的病毒,对全球猪群构成威胁。迄今为止,三方基序 5(TRIM5)在病毒复制中的作用尚未得到评估。据报道,TRIM5 可通过促进由视黄酸诱导基因 I(RIG-I)介导的 I 型干扰素(IFN)抗病毒反应来抑制 SVA 的复制。TRIM5 在 SVA 感染细胞中的表达明显上调,TRIM5 的过表达抑制了病毒复制,促进了 IFN-α、IFN-β、白细胞介素-1β(IL-1β)、IL-6 和 IL-18 的表达。相反,干扰 TRIM5 的表达则会产生相反的效果。病毒吸附和进入试验表明,TRIM5 不影响 SVA 的吸附,但抑制其进入。此外,TRIM5 还能促进 RIG-I 和 RIG-I 介导的 IFNs 以及促炎细胞因子的表达,抑制 TRIM5 的表达也能证明这种作用。这些发现扩大了人们对抑制 SVA 复制的宿主因素的认识范围,并表明靶向 TRIM5 可能有助于开发抗击 SVA 的新药。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
TRIM5 inhibits the replication of Senecavirus A by promoting the RIG-I-mediated type I interferon antiviral response.

Senecavirus A (SVA) is an emerging virus that poses a threat to swine herds worldwide. To date, the role of tripartite motif 5 (TRIM5) in the replication of viruses has not been evaluated. Here, TRIM5 was reported to inhibit SVA replication by promoting the type I interferon (IFN) antiviral response mediated by retinoic acid-inducible gene I (RIG-I). TRIM5 expression was significantly upregulated in SVA-infected cells, and TRIM5 overexpression inhibited viral replication and promoted IFN-α, IFN-β, interleukin-1beta (IL-1β), IL-6, and IL-18 expression. Conversely, interfering with the expression of TRIM5 had the opposite effect. Viral adsorption and entry assays showed that TRIM5 did not affect the adsorption of SVA but inhibited its entry. In addition, TRIM5 promoted the expression of RIG-I and RIG-I-mediated IFNs and proinflammatory cytokines, and this effect was also proven by inhibiting the expression of TRIM5. These findings expand the scope of knowledge on host factors inhibiting the replication of SVA and indicate that targeting TRIM5 may aid in the development of new agents against SVA.

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来源期刊
Veterinary Research
Veterinary Research 农林科学-兽医学
CiteScore
7.00
自引率
4.50%
发文量
92
审稿时长
3 months
期刊介绍: Veterinary Research is an open access journal that publishes high quality and novel research and review articles focusing on all aspects of infectious diseases and host-pathogen interaction in animals.
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