从聚合进化中发现含硒抗氧化剂卵硒醇

IF 19.2 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Chase M. Kayrouz, Kendra A. Ireland, Vanessa Y. Ying, Katherine M. Davis, Mohammad R. Seyedsayamdost
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引用次数: 0

摘要

硒是人体必需的微量元素,但它在生物学中的存在仅限于蛋白质和核酸生物聚合物。最近发现的含硒小分子生物合成途径表明,还有一个更大的硒代谢物家族有待发现。在这里,我们利用生物信息学搜索策略,通过绘制复合活性位点图案,发现了最近进化的、丰富的、未表征的金属酶分支,并预测它们参与了硒代谢物的生物合成。生化研究证实了这一预测,并表明这些酶在组氨酸上形成了不寻常的 C-Se键,从而产生了一种独特的硒代谢物和强效抗氧化剂,我们称之为卵硒醇。我们的工作不仅为了解这类酶的进化和 C-Se 键形成的结构基础提供了见解,还为未来绘制微生物硒代谢组提供了蓝图。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Discovery of the selenium-containing antioxidant ovoselenol derived from convergent evolution

Discovery of the selenium-containing antioxidant ovoselenol derived from convergent evolution

Discovery of the selenium-containing antioxidant ovoselenol derived from convergent evolution
Selenium is an essential micronutrient, but its presence in biology has been limited to protein and nucleic acid biopolymers. The recent identification of a biosynthetic pathway for selenium-containing small molecules suggests that there is a larger family of selenometabolites that remains to be discovered. Here we identify a recently evolved branch of abundant and uncharacterized metalloenzymes that we predict are involved in selenometabolite biosynthesis using a bioinformatic search strategy that relies on the mapping of composite active site motifs. Biochemical studies confirm this prediction and show that these enzymes form an unusual C–Se bond onto histidine, thus giving rise to a distinct selenometabolite and potent antioxidant that we have termed ovoselenol. Aside from providing insights into the evolution of this enzyme class and the structural basis of C–Se bond formation, our work offers a blueprint for charting the microbial selenometabolome in the future. Although biosynthetic pathways of selenium-containing macromolecules have been known for decades, pathways for specific incorporation of selenium into small molecules have only recently begun to be uncovered. Now the selenometabolome is expanded further through the discovery and biosynthetic elucidation of ovoselenol, a selenium-containing antioxidant found in marine microorganisms.
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来源期刊
Nature chemistry
Nature chemistry 化学-化学综合
CiteScore
29.60
自引率
1.40%
发文量
226
审稿时长
1.7 months
期刊介绍: Nature Chemistry is a monthly journal that publishes groundbreaking and significant research in all areas of chemistry. It covers traditional subjects such as analytical, inorganic, organic, and physical chemistry, as well as a wide range of other topics including catalysis, computational and theoretical chemistry, and environmental chemistry. The journal also features interdisciplinary research at the interface of chemistry with biology, materials science, nanotechnology, and physics. Manuscripts detailing such multidisciplinary work are encouraged, as long as the central theme pertains to chemistry. Aside from primary research, Nature Chemistry publishes review articles, news and views, research highlights from other journals, commentaries, book reviews, correspondence, and analysis of the broader chemical landscape. It also addresses crucial issues related to education, funding, policy, intellectual property, and the societal impact of chemistry. Nature Chemistry is dedicated to ensuring the highest standards of original research through a fair and rigorous review process. It offers authors maximum visibility for their papers, access to a broad readership, exceptional copy editing and production standards, rapid publication, and independence from academic societies and other vested interests. Overall, Nature Chemistry aims to be the authoritative voice of the global chemical community.
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