溶剂和介质对 cranad-2 和 cranad-58 光物理学的影响

IF 4.1 3区 化学 Q2 CHEMISTRY, PHYSICAL
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引用次数: 0

摘要

阿尔茨海默病(AD)是与年龄相关的认知退化中最常见的一种,其特征是长期的退行性损伤、认知功能障碍以及逻辑思维、知识获取和人际沟通方面的严重缺陷。老年痴呆症的主要生物标志物是β淀粉样蛋白(Aβ)的出现和聚集。Cranad 衍生物是一种荧光探针,能够对 Aβ 聚集体进行检测、量化和成像。在这项研究中,采用了大量溶剂和多种微生物系统,研究了溶剂和微异构环境对 CRANAD-2 和 CRANAD-58 光物理行为的影响。吸收光谱和荧光光谱都表现出很大的溶解变色效应,导致显著的斯托克斯偏移。应用线性溶解能关系将荧光光谱最大值和斯托克斯位移与微观溶剂参数相关联,表明激发过程中分子内电荷转移显著,激发态偶极矩的增加也证实了这一点。一般来说,在大多数溶剂中,CRANAD-2 的荧光量子产率都超过了 CRANAD-58,在极性溶剂中数值较低,而在非极性溶剂中数值较大。将 CRANAD-2 和 CRANAD-58 引入胶束和囊泡溶液中会显著提高荧光发射强度,并伴随着荧光最大值的蓝移。在微均质系统中观察到的荧光最大值与报道的 CRANAD 衍生物与可溶性或聚集的 Aβ 淀粉样蛋白之间的相互作用的荧光最大值非常接近,这可能会限制 "体内 "Aβ 检测试验的效果,因为在生物介质中的非极性微环境中定位 CRANAD 衍生物可能会干扰淀粉样蛋白纤维的检测。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Solvent and media effects on the photophysics of cranad-2 and cranad-58

Solvent and media effects on the photophysics of cranad-2 and cranad-58

Alzheimer’s disease (AD) represents the most widespread form of age-related cognitive degeneration, characterized by long-term degenerative damage, cognitive dysfunction, and profound deficits in logical thinking, knowledge acquisition, and interpersonal communication. A principal biomarker of AD involves the emergence and aggregation of β-amyloid (Aβ). Cranad derivatives are fluorescent probes capable of detecting, quantifying, and imaging Aβ aggregates. In this work, the effect of solvent and microheterogeneous environments on the photophysical behavior of CRANAD-2 and CRANAD-58 was investigated employing a large solvent set and several microorganized systems. Both the absorption and fluorescence spectra exhibit a substantial solvatochromic effect, resulting in significant Stokes shifts. Application of linear solvation energy relationships to correlate the fluorescence spectra maxima and the Stokes shift with microscopic solvent parameters suggests significant intramolecular charge transfer during the excitation, as corroborated by the increased dipole moment in the excited state. Generally, fluorescence quantum yields determined for CRANAD-2 exceed those of CRANAD-58 in most solvents, with low values in polar solvents and bigger values in non-polar solvents. Introduction of CRANAD-2 and CRANAD-58 into micellar and vesicular solutions notably augments the fluorescence emission intensity, accompanied by a blue shift in the fluorescence maxima. The fluorescence maxima values observed within microheterogeneous systems closely parallel those reported for interactions between CRANAD derivatives and soluble or aggregated Aβ amyloids which potentially constrains the efficacy of “in vivo” Aβ detection trials, because localization of CRANAD derivatives in non-polar microenvironments present in biological media could interfere with the detection of amyloid fibers.

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来源期刊
CiteScore
7.90
自引率
7.00%
发文量
580
审稿时长
48 days
期刊介绍: JPPA publishes the results of fundamental studies on all aspects of chemical phenomena induced by interactions between light and molecules/matter of all kinds. All systems capable of being described at the molecular or integrated multimolecular level are appropriate for the journal. This includes all molecular chemical species as well as biomolecular, supramolecular, polymer and other macromolecular systems, as well as solid state photochemistry. In addition, the journal publishes studies of semiconductor and other photoactive organic and inorganic materials, photocatalysis (organic, inorganic, supramolecular and superconductor). The scope includes condensed and gas phase photochemistry, as well as synchrotron radiation chemistry. A broad range of processes and techniques in photochemistry are covered such as light induced energy, electron and proton transfer; nonlinear photochemical behavior; mechanistic investigation of photochemical reactions and identification of the products of photochemical reactions; quantum yield determinations and measurements of rate constants for primary and secondary photochemical processes; steady-state and time-resolved emission, ultrafast spectroscopic methods, single molecule spectroscopy, time resolved X-ray diffraction, luminescence microscopy, and scattering spectroscopy applied to photochemistry. Papers in emerging and applied areas such as luminescent sensors, electroluminescence, solar energy conversion, atmospheric photochemistry, environmental remediation, and related photocatalytic chemistry are also welcome.
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