{"title":"溶剂和介质对 cranad-2 和 cranad-58 光物理学的影响","authors":"","doi":"10.1016/j.jphotochem.2024.115935","DOIUrl":null,"url":null,"abstract":"<div><p>Alzheimer’s disease (AD) represents the most widespread form of age-related cognitive degeneration, characterized by long-term degenerative damage, cognitive dysfunction, and profound deficits in logical thinking, knowledge acquisition, and interpersonal communication. A principal biomarker of AD involves the emergence and aggregation of β-amyloid (Aβ). Cranad derivatives are fluorescent probes capable of detecting, quantifying, and imaging Aβ aggregates. In this work, the effect of solvent and microheterogeneous environments on the photophysical behavior of CRANAD-2 and CRANAD-58 was investigated employing a large solvent set and several microorganized systems. Both the absorption and fluorescence spectra exhibit a substantial solvatochromic effect, resulting in significant Stokes shifts. Application of linear solvation energy relationships to correlate the fluorescence spectra maxima and the Stokes shift with microscopic solvent parameters suggests significant intramolecular charge transfer during the excitation, as corroborated by the increased dipole moment in the excited state. Generally, fluorescence quantum yields determined for CRANAD-2 exceed those of CRANAD-58 in most solvents, with low values in polar solvents and bigger values in non-polar solvents. Introduction of CRANAD-2 and CRANAD-58 into micellar and vesicular solutions notably augments the fluorescence emission intensity, accompanied by a blue shift in the fluorescence maxima. The fluorescence maxima values observed within microheterogeneous systems closely parallel those reported for interactions between CRANAD derivatives and soluble or aggregated Aβ amyloids which potentially constrains the efficacy of “<em>in vivo</em>” Aβ detection trials, because localization of CRANAD derivatives in non-polar microenvironments present in biological media could interfere with the detection of amyloid fibers.</p></div>","PeriodicalId":16782,"journal":{"name":"Journal of Photochemistry and Photobiology A-chemistry","volume":null,"pages":null},"PeriodicalIF":4.1000,"publicationDate":"2024-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1010603024004799/pdfft?md5=37641c986faa41646e4e5b4acc6c8bbc&pid=1-s2.0-S1010603024004799-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Solvent and media effects on the photophysics of cranad-2 and cranad-58\",\"authors\":\"\",\"doi\":\"10.1016/j.jphotochem.2024.115935\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Alzheimer’s disease (AD) represents the most widespread form of age-related cognitive degeneration, characterized by long-term degenerative damage, cognitive dysfunction, and profound deficits in logical thinking, knowledge acquisition, and interpersonal communication. A principal biomarker of AD involves the emergence and aggregation of β-amyloid (Aβ). Cranad derivatives are fluorescent probes capable of detecting, quantifying, and imaging Aβ aggregates. In this work, the effect of solvent and microheterogeneous environments on the photophysical behavior of CRANAD-2 and CRANAD-58 was investigated employing a large solvent set and several microorganized systems. Both the absorption and fluorescence spectra exhibit a substantial solvatochromic effect, resulting in significant Stokes shifts. Application of linear solvation energy relationships to correlate the fluorescence spectra maxima and the Stokes shift with microscopic solvent parameters suggests significant intramolecular charge transfer during the excitation, as corroborated by the increased dipole moment in the excited state. Generally, fluorescence quantum yields determined for CRANAD-2 exceed those of CRANAD-58 in most solvents, with low values in polar solvents and bigger values in non-polar solvents. Introduction of CRANAD-2 and CRANAD-58 into micellar and vesicular solutions notably augments the fluorescence emission intensity, accompanied by a blue shift in the fluorescence maxima. The fluorescence maxima values observed within microheterogeneous systems closely parallel those reported for interactions between CRANAD derivatives and soluble or aggregated Aβ amyloids which potentially constrains the efficacy of “<em>in vivo</em>” Aβ detection trials, because localization of CRANAD derivatives in non-polar microenvironments present in biological media could interfere with the detection of amyloid fibers.</p></div>\",\"PeriodicalId\":16782,\"journal\":{\"name\":\"Journal of Photochemistry and Photobiology A-chemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2024-08-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1010603024004799/pdfft?md5=37641c986faa41646e4e5b4acc6c8bbc&pid=1-s2.0-S1010603024004799-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Photochemistry and Photobiology A-chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1010603024004799\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, PHYSICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Photochemistry and Photobiology A-chemistry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1010603024004799","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
Solvent and media effects on the photophysics of cranad-2 and cranad-58
Alzheimer’s disease (AD) represents the most widespread form of age-related cognitive degeneration, characterized by long-term degenerative damage, cognitive dysfunction, and profound deficits in logical thinking, knowledge acquisition, and interpersonal communication. A principal biomarker of AD involves the emergence and aggregation of β-amyloid (Aβ). Cranad derivatives are fluorescent probes capable of detecting, quantifying, and imaging Aβ aggregates. In this work, the effect of solvent and microheterogeneous environments on the photophysical behavior of CRANAD-2 and CRANAD-58 was investigated employing a large solvent set and several microorganized systems. Both the absorption and fluorescence spectra exhibit a substantial solvatochromic effect, resulting in significant Stokes shifts. Application of linear solvation energy relationships to correlate the fluorescence spectra maxima and the Stokes shift with microscopic solvent parameters suggests significant intramolecular charge transfer during the excitation, as corroborated by the increased dipole moment in the excited state. Generally, fluorescence quantum yields determined for CRANAD-2 exceed those of CRANAD-58 in most solvents, with low values in polar solvents and bigger values in non-polar solvents. Introduction of CRANAD-2 and CRANAD-58 into micellar and vesicular solutions notably augments the fluorescence emission intensity, accompanied by a blue shift in the fluorescence maxima. The fluorescence maxima values observed within microheterogeneous systems closely parallel those reported for interactions between CRANAD derivatives and soluble or aggregated Aβ amyloids which potentially constrains the efficacy of “in vivo” Aβ detection trials, because localization of CRANAD derivatives in non-polar microenvironments present in biological media could interfere with the detection of amyloid fibers.
期刊介绍:
JPPA publishes the results of fundamental studies on all aspects of chemical phenomena induced by interactions between light and molecules/matter of all kinds.
All systems capable of being described at the molecular or integrated multimolecular level are appropriate for the journal. This includes all molecular chemical species as well as biomolecular, supramolecular, polymer and other macromolecular systems, as well as solid state photochemistry. In addition, the journal publishes studies of semiconductor and other photoactive organic and inorganic materials, photocatalysis (organic, inorganic, supramolecular and superconductor).
The scope includes condensed and gas phase photochemistry, as well as synchrotron radiation chemistry. A broad range of processes and techniques in photochemistry are covered such as light induced energy, electron and proton transfer; nonlinear photochemical behavior; mechanistic investigation of photochemical reactions and identification of the products of photochemical reactions; quantum yield determinations and measurements of rate constants for primary and secondary photochemical processes; steady-state and time-resolved emission, ultrafast spectroscopic methods, single molecule spectroscopy, time resolved X-ray diffraction, luminescence microscopy, and scattering spectroscopy applied to photochemistry. Papers in emerging and applied areas such as luminescent sensors, electroluminescence, solar energy conversion, atmospheric photochemistry, environmental remediation, and related photocatalytic chemistry are also welcome.