利用番茄悬浮培养物提高α-生育酚产量的诱导和前体诱导方法

IF 5.4 Q1 PLANT SCIENCES
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引用次数: 0

摘要

诱导和前体饲喂是提高植物细胞悬浮培养物中生物活性化合物合成的有效策略。本研究旨在探索一种高效的诱导和前体喂养方案及其对诱导番茄悬浮细胞培养物中α-生育酚积累的影响。用不同的诱导剂(茉莉酸甲酯、水杨酸和酵母提取物)和前体(高柑橘酸、酪氨酸、羟基丙酮酸和植物醇)处理番茄悬浮细胞培养物,并研究其对α-生育酚产生的影响。在茉莉酸甲酯处理后的第 5 天,观察到 α-生育酚显著增加,与对照组相比增加了 17.7 倍。150 μM Homogentisic acid + 150 μM Phytol 的前体组合处理显示,与未处理的对照组相比,α-生育酚在第 10 天增加了 22 倍。这些结果表明,悬浮培养物与最佳前体喂养和诱导剂相结合,提高了具有重要经济价值的番茄细胞培养物中 α-生育酚的产量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Elicitation and precursor induced approaches for the enhancement of α−tocopherol production using suspension cultures of Solanum lycopersicum

Elicitation and precursor feeding are the effective strategies for enhancing the synthesis of bioactive compounds in plant cell suspension cultures. The present study aimed to explore an efficient elicitation and precursor feeding protocol and its effect on inducing the accumulation of α-tocopherol in Solanum lycopersicum (tomato) suspension cell culture. The tomato cell suspension cultures were treated with different elicitors (Methyl Jasmonate, Salicylic acid and Yeast extract) and precursors (Homogentisic acid, Tyrosine, Hydroxypyruvic acid and Phytol) and the effect of α-tocopherol production was studied. Significant increase in the α-tocopherol was observed on day 5 upon methyl jasmonate treatment which represented 17.7 fold increase in comparison to the control. The treatment of precursor in combination viz., 150 μM Homogentisic acid + 150 μM Phytol showed the maximum enhancement of α-tocopherol up to 22 fold on day 10 compared to the untreated control. These results suggested that the suspension cultures combining with the optimal precursor feeding and elicitors enhanced the production of α-tocopherol in economically important tomato cell cultures.

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来源期刊
Current Plant Biology
Current Plant Biology Agricultural and Biological Sciences-Plant Science
CiteScore
10.90
自引率
1.90%
发文量
32
审稿时长
50 days
期刊介绍: Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.
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