{"title":"NaCl 胁迫下离体培养的 Gynura divaricata (L.) DC. 的生理特征和反应模式转录组分析","authors":"","doi":"10.1016/j.cpb.2024.100379","DOIUrl":null,"url":null,"abstract":"<div><p>Soil salinity, a major environmental stress, restricts agricultural production worldwide. <em>Gynura divaricata</em> (L.) DC. is widely cultivated on tropical islands in China and has both edible and medicinal value. NaCl stress and growth indicators, antioxidant enzyme activity, as well as MDA, proline, and soluble sugar content, were determined. Based on the transcriptomic data of tissue-cultured <em>G. divaricata</em> plantlets grown in control (0 mM NaCl) and salt stress (50 and 200 mM NaCl) conditions, gene expression patterns were examined. KEGG enrichment analysis of differentially expressed genes indicated significant enrichment of plant hormone signaling, MAPK signaling, and starch and sucrose metabolism pathways. These findings allowed key biological pathways and salt stress-responsive genes to be identified, thus providing a molecular basis for breeding salt-tolerant <em>G. divaricata</em> varieties. This transcriptomic analysis revealed a complex tolerance mechanism of <em>G. divaricata</em> in response to NaCl, laying a foundation for screening and cloning key genes related to NaCl tolerance and studying their interactions. These findings would allow the molecular basis of NaCl tolerance to be explored, with the aim of breeding NaCl-tolerant varieties.</p></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":null,"pages":null},"PeriodicalIF":5.4000,"publicationDate":"2024-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214662824000616/pdfft?md5=936a50b7a3cd144f41f85804ff215d9c&pid=1-s2.0-S2214662824000616-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Physiological characteristics and transcriptomic analysis of response patterns of Gynura divaricata (L.) DC. cultured in vitro under NaCl stress\",\"authors\":\"\",\"doi\":\"10.1016/j.cpb.2024.100379\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Soil salinity, a major environmental stress, restricts agricultural production worldwide. <em>Gynura divaricata</em> (L.) DC. is widely cultivated on tropical islands in China and has both edible and medicinal value. NaCl stress and growth indicators, antioxidant enzyme activity, as well as MDA, proline, and soluble sugar content, were determined. Based on the transcriptomic data of tissue-cultured <em>G. divaricata</em> plantlets grown in control (0 mM NaCl) and salt stress (50 and 200 mM NaCl) conditions, gene expression patterns were examined. KEGG enrichment analysis of differentially expressed genes indicated significant enrichment of plant hormone signaling, MAPK signaling, and starch and sucrose metabolism pathways. These findings allowed key biological pathways and salt stress-responsive genes to be identified, thus providing a molecular basis for breeding salt-tolerant <em>G. divaricata</em> varieties. This transcriptomic analysis revealed a complex tolerance mechanism of <em>G. divaricata</em> in response to NaCl, laying a foundation for screening and cloning key genes related to NaCl tolerance and studying their interactions. These findings would allow the molecular basis of NaCl tolerance to be explored, with the aim of breeding NaCl-tolerant varieties.</p></div>\",\"PeriodicalId\":38090,\"journal\":{\"name\":\"Current Plant Biology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.4000,\"publicationDate\":\"2024-08-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2214662824000616/pdfft?md5=936a50b7a3cd144f41f85804ff215d9c&pid=1-s2.0-S2214662824000616-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Plant Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2214662824000616\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Plant Biology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214662824000616","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
土壤盐碱化是一种主要的环境压力,制约着全世界的农业生产。Gynura divaricata (L.) DC. 在中国热带岛屿上广泛种植,具有食用和药用价值。研究测定了NaCl胁迫和生长指标、抗氧化酶活性以及MDA、脯氨酸和可溶性糖含量。根据在对照(0 mM NaCl)和盐胁迫(50 mM NaCl 和 200 mM NaCl)条件下生长的组织培养小株 G. divaricata 的转录组数据,研究了基因表达模式。对差异表达基因的 KEGG 富集分析表明,植物激素信号转导、MAPK 信号转导以及淀粉和蔗糖代谢途径的基因表达明显增加。这些发现确定了关键生物通路和盐胁迫响应基因,从而为培育耐盐 G. divaricata 品种提供了分子基础。该转录组分析揭示了 G. divaricata 对 NaCl 的复杂耐受机制,为筛选和克隆与耐 NaCl 相关的关键基因并研究它们之间的相互作用奠定了基础。这些发现将有助于探索耐NaCl的分子基础,从而培育出耐NaCl的品种。
Physiological characteristics and transcriptomic analysis of response patterns of Gynura divaricata (L.) DC. cultured in vitro under NaCl stress
Soil salinity, a major environmental stress, restricts agricultural production worldwide. Gynura divaricata (L.) DC. is widely cultivated on tropical islands in China and has both edible and medicinal value. NaCl stress and growth indicators, antioxidant enzyme activity, as well as MDA, proline, and soluble sugar content, were determined. Based on the transcriptomic data of tissue-cultured G. divaricata plantlets grown in control (0 mM NaCl) and salt stress (50 and 200 mM NaCl) conditions, gene expression patterns were examined. KEGG enrichment analysis of differentially expressed genes indicated significant enrichment of plant hormone signaling, MAPK signaling, and starch and sucrose metabolism pathways. These findings allowed key biological pathways and salt stress-responsive genes to be identified, thus providing a molecular basis for breeding salt-tolerant G. divaricata varieties. This transcriptomic analysis revealed a complex tolerance mechanism of G. divaricata in response to NaCl, laying a foundation for screening and cloning key genes related to NaCl tolerance and studying their interactions. These findings would allow the molecular basis of NaCl tolerance to be explored, with the aim of breeding NaCl-tolerant varieties.
期刊介绍:
Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.