Y染色体基因与间隔时间相互作用,调节雄性黑腹果蝇的交配持续时间

IF 1 Q4 GENETICS & HEREDITY
Yongwen Huang , Alex Kwan , Woo Jae Kim
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引用次数: 0

摘要

我们探讨了 Y 染色体基因在调节雄性果蝇交配行为和间隔时间方面未被充分研究的作用。我们的发现揭示了这些基因对交配持续时间的重要影响,而交配持续时间是性选择和繁殖成功的一个关键方面。通过利用缺乏 Y 染色体的 XO 雄性和 RNA 干扰(RNAi)技术敲除特定的 Y 染色体基因,我们证明 Y 染色体及其基因 WDY 和 CCY 对于产生较长交配持续时间(LMD)和较短交配持续时间(SMD)行为至关重要。值得注意的是,神经元敲除在神经元和神经胶质细胞中高度表达的 Ppr-Y 基因会导致求偶和交配行为的严重破坏,而不会影响生育能力。利用苍蝇 SCope scRNA-seq 数据平台,我们发现包括 kl-3、kl-5、WDY 和 PRY 在内的几个 Y 染色体基因在 fru 阳性神经元中优先表达,这表明它们在雄性特异性神经元群体中发挥作用。我们的工作不仅加深了人们对Y染色体在复杂交配行为中的作用的理解,而且为今后研究果蝇性二型和生殖策略的分子机制奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Y chromosome genes interplay with interval timing in regulating mating duration of male Drosophila melanogaster

We explore the understudied role of Y chromosome genes in modulating mating behaviors and interval timing in male fruit flies. Our findings reveal a significant impact of these genes on mating duration, a critical aspect of sexual selection and reproductive success. Through the use of XO males lacking a Y chromosome and RNA interference (RNAi) techniques to knockdown specific Y chromosome genes, we demonstrate that the Y chromosome and its genes WDY and CCY are essential for the generation of Longer-Mating-Duration (LMD) and Shorter-Mating-Duration (SMD) behaviors. Notably, the neuronal knockdown of Ppr-Y, a gene highly expressed in both neuronal and glial cells, leads to profound disruptions in courtship and mating behaviors without affecting fertility. Utilizing the fly SCope scRNA-seq data platform, we identified that several Y chromosome genes, including kl-3, kl-5, WDY, and PRY, are preferentially expressed in fru-positive neurons, suggesting a role in male-specific neuronal populations. Our work not only advances the understanding of the Y chromosome's contribution to complex mating behaviors but also sets the stage for future investigations into the molecular mechanisms underlying sexual dimorphism and reproductive strategies in Drosophila.

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来源期刊
Gene Reports
Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
3.30
自引率
7.70%
发文量
246
审稿时长
49 days
期刊介绍: Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.
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