{"title":"毛细管微型样本中血小板计数的自动方法。","authors":"Caroline Vasard Boesen, Vibeke Staun Christensen, Klaus Rosenkilde Jensen, Anja Reinert Hansen, Claus Vinter Bødker Hviid","doi":"10.1111/ijlh.14354","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Introduction</h3>\n \n <p>We aimed to develop an automated, low-volume method for thrombocyte counting in capillary blood using the Sysmex predilution (PD) mode.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Microsamples were prepared by resuspension of 50 μL blood in 300 μL DCL CellPack. Thrombocyte counting was done in the impedance (PLT-I) and fluorescence (PLT-F) channels. The imprecision and bias was evaluated in >394 microsamples from adult blood. Preanalytical factors (skin-piercing, storage, and transportation in our pneumatic tube system) was assessed, and studies on pediatric microsamples were made for comparison. The improvement in analytical quality and turnaround time was examined.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>For PLT-F, the imprecision was 1.1%–3.7%, and the bias was 10.1% (95% CI: 8.8–11.3). After skin-piercing, the bias was 8.1% (95% CI: 5.6–10.6) and the imprecision 1.9% (95% CI: 1.3–2.5). Thrombocyte counts kept stable after 4 h at room temperature (94.8% [95% CI: 93.2–96.4]) and after pneumatic tube transportation [6.7% (95% CI: 4.8–8.6)]. The bias of the PD mode for pediatric microsamples was 13.0% (95% CI: −8.4–34.4) in the PLT-F channel. The automated method had a considerably lower imprecision than the existing manual thrombocyte counting method and reduced turnaround times.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>The automated microsample method offers a low-volume alternative for measurement of thrombocytes. The method appears useful also in pediatric samples.</p>\n </section>\n </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"46 6","pages":"1044-1051"},"PeriodicalIF":2.2000,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ijlh.14354","citationCount":"0","resultStr":"{\"title\":\"An automated method for thrombocyte counting in capillary microsamples\",\"authors\":\"Caroline Vasard Boesen, Vibeke Staun Christensen, Klaus Rosenkilde Jensen, Anja Reinert Hansen, Claus Vinter Bødker Hviid\",\"doi\":\"10.1111/ijlh.14354\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Introduction</h3>\\n \\n <p>We aimed to develop an automated, low-volume method for thrombocyte counting in capillary blood using the Sysmex predilution (PD) mode.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>Microsamples were prepared by resuspension of 50 μL blood in 300 μL DCL CellPack. Thrombocyte counting was done in the impedance (PLT-I) and fluorescence (PLT-F) channels. The imprecision and bias was evaluated in >394 microsamples from adult blood. Preanalytical factors (skin-piercing, storage, and transportation in our pneumatic tube system) was assessed, and studies on pediatric microsamples were made for comparison. The improvement in analytical quality and turnaround time was examined.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>For PLT-F, the imprecision was 1.1%–3.7%, and the bias was 10.1% (95% CI: 8.8–11.3). After skin-piercing, the bias was 8.1% (95% CI: 5.6–10.6) and the imprecision 1.9% (95% CI: 1.3–2.5). Thrombocyte counts kept stable after 4 h at room temperature (94.8% [95% CI: 93.2–96.4]) and after pneumatic tube transportation [6.7% (95% CI: 4.8–8.6)]. The bias of the PD mode for pediatric microsamples was 13.0% (95% CI: −8.4–34.4) in the PLT-F channel. The automated method had a considerably lower imprecision than the existing manual thrombocyte counting method and reduced turnaround times.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>The automated microsample method offers a low-volume alternative for measurement of thrombocytes. The method appears useful also in pediatric samples.</p>\\n </section>\\n </div>\",\"PeriodicalId\":14120,\"journal\":{\"name\":\"International Journal of Laboratory Hematology\",\"volume\":\"46 6\",\"pages\":\"1044-1051\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-08-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ijlh.14354\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Laboratory Hematology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/ijlh.14354\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"HEMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Laboratory Hematology","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/ijlh.14354","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"HEMATOLOGY","Score":null,"Total":0}
An automated method for thrombocyte counting in capillary microsamples
Introduction
We aimed to develop an automated, low-volume method for thrombocyte counting in capillary blood using the Sysmex predilution (PD) mode.
Methods
Microsamples were prepared by resuspension of 50 μL blood in 300 μL DCL CellPack. Thrombocyte counting was done in the impedance (PLT-I) and fluorescence (PLT-F) channels. The imprecision and bias was evaluated in >394 microsamples from adult blood. Preanalytical factors (skin-piercing, storage, and transportation in our pneumatic tube system) was assessed, and studies on pediatric microsamples were made for comparison. The improvement in analytical quality and turnaround time was examined.
Results
For PLT-F, the imprecision was 1.1%–3.7%, and the bias was 10.1% (95% CI: 8.8–11.3). After skin-piercing, the bias was 8.1% (95% CI: 5.6–10.6) and the imprecision 1.9% (95% CI: 1.3–2.5). Thrombocyte counts kept stable after 4 h at room temperature (94.8% [95% CI: 93.2–96.4]) and after pneumatic tube transportation [6.7% (95% CI: 4.8–8.6)]. The bias of the PD mode for pediatric microsamples was 13.0% (95% CI: −8.4–34.4) in the PLT-F channel. The automated method had a considerably lower imprecision than the existing manual thrombocyte counting method and reduced turnaround times.
Conclusion
The automated microsample method offers a low-volume alternative for measurement of thrombocytes. The method appears useful also in pediatric samples.
期刊介绍：
The International Journal of Laboratory Hematology provides a forum for the communication of new developments, research topics and the practice of laboratory haematology.
The journal publishes invited reviews, full length original articles, and correspondence.
The International Journal of Laboratory Hematology is the official journal of the International Society for Laboratory Hematology, which addresses the following sub-disciplines: cellular analysis, flow cytometry, haemostasis and thrombosis, molecular diagnostics, haematology informatics, haemoglobinopathies, point of care testing, standards and guidelines.
The journal was launched in 2006 as the successor to Clinical and Laboratory Hematology, which was first published in 1979. An active and positive editorial policy ensures that work of a high scientific standard is reported, in order to bridge the gap between practical and academic aspects of laboratory haematology.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
