{"title":"来自南非木质电线杆的一种新的 Paecilomyces。","authors":"C M Visagie, E M Cruywagen, T A Duong","doi":"10.3114/fuse.2024.13.10","DOIUrl":null,"url":null,"abstract":"<p><p>During a survey of fungi on electricity utility poles in South Africa, a diverse range of fungi were discovered. <i>Paecilomyces</i> was frequently isolated, with five species identified using ITS and β-tubulin (<i>BenA</i>) sequences. These were <i>P. brunneolus</i>, <i>P. dactylethromorphus</i>, <i>P. lecythidis</i>, <i>P. paravariotii</i> and a potential new species. The genomes of 30 of these strains were subsequently sequenced and used in a phylogenomic analysis with 45 previously published genomes of the genus. Phylogenetic analyses were also conducted using ITS, <i>BenA</i>, calmodulin (<i>CaM</i>), RNA polymerase II second largest subunit (<i>RPB2</i>), RNA polymerase II largest subunit (<i>RPB1</i>), the genes coding for the theta subunit of the TCP-1 chaperonin complex (<i>Cct8</i>), and for a putative ribosome biogenesis protein (<i>Tsr1</i>). Both phylogenomic and phylogenetic analyses supported the 15 <i>Paecilomyces</i> species currently accepted and confirmed the novelty of the new species, which we describe as <i>P. lignorum</i>. The latter is the sister species of <i>P. brunneolus</i> and belongs to a clade also containing <i>P. variotii</i> and <i>P. paravariotii</i>. Morphologically, the new species produces longer ellipsoidal conidia and grows more restricted on malt extract agar at 30 °C compared to its closest relatives. <b>Citation:</b> Visagie CM, Cruywagen EM, Duong TA (2024). A new <i>Paecilomyces</i> from wooden utility poles in South Africa. <i>Fungal Systematics and Evolution</i> <b>13</b>: 163-181. doi: 10.3114/fuse.2024.13.10.</p>","PeriodicalId":73121,"journal":{"name":"Fungal systematics and evolution","volume":"13 ","pages":"163-181"},"PeriodicalIF":0.0000,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11319801/pdf/","citationCount":"0","resultStr":"{\"title\":\"A new <i>Paecilomyces</i> from wooden utility poles in South Africa.\",\"authors\":\"C M Visagie, E M Cruywagen, T A Duong\",\"doi\":\"10.3114/fuse.2024.13.10\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>During a survey of fungi on electricity utility poles in South Africa, a diverse range of fungi were discovered. <i>Paecilomyces</i> was frequently isolated, with five species identified using ITS and β-tubulin (<i>BenA</i>) sequences. These were <i>P. brunneolus</i>, <i>P. dactylethromorphus</i>, <i>P. lecythidis</i>, <i>P. paravariotii</i> and a potential new species. The genomes of 30 of these strains were subsequently sequenced and used in a phylogenomic analysis with 45 previously published genomes of the genus. Phylogenetic analyses were also conducted using ITS, <i>BenA</i>, calmodulin (<i>CaM</i>), RNA polymerase II second largest subunit (<i>RPB2</i>), RNA polymerase II largest subunit (<i>RPB1</i>), the genes coding for the theta subunit of the TCP-1 chaperonin complex (<i>Cct8</i>), and for a putative ribosome biogenesis protein (<i>Tsr1</i>). Both phylogenomic and phylogenetic analyses supported the 15 <i>Paecilomyces</i> species currently accepted and confirmed the novelty of the new species, which we describe as <i>P. lignorum</i>. The latter is the sister species of <i>P. brunneolus</i> and belongs to a clade also containing <i>P. variotii</i> and <i>P. paravariotii</i>. Morphologically, the new species produces longer ellipsoidal conidia and grows more restricted on malt extract agar at 30 °C compared to its closest relatives. <b>Citation:</b> Visagie CM, Cruywagen EM, Duong TA (2024). A new <i>Paecilomyces</i> from wooden utility poles in South Africa. <i>Fungal Systematics and Evolution</i> <b>13</b>: 163-181. doi: 10.3114/fuse.2024.13.10.</p>\",\"PeriodicalId\":73121,\"journal\":{\"name\":\"Fungal systematics and evolution\",\"volume\":\"13 \",\"pages\":\"163-181\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11319801/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Fungal systematics and evolution\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3114/fuse.2024.13.10\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/6/7 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fungal systematics and evolution","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3114/fuse.2024.13.10","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/6/7 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
在对南非电线杆上的真菌进行调查期间,发现了多种真菌。通过 ITS 和 β-tubulin(BenA)序列确定了 5 个物种。这些菌种分别是布鲁内罗氏真菌(P. brunneolus)、半木质化真菌(P. dactylethromorphus)、lecythidis 真菌(P. lecythidis)、paravariotii 真菌和一个潜在的新菌种。随后对其中 30 个菌株的基因组进行了测序,并与之前发表的 45 个该属的基因组进行了系统发生组分析。系统进化分析还使用了 ITS、BenA、钙调蛋白(CaM)、RNA 聚合酶 II 第二大亚基(RPB2)、RNA 聚合酶 II 最大亚基(RPB1)、TCP-1 合子蛋白复合物(Cct8)θ 亚基的编码基因以及推测的核糖体生物生成蛋白(Tsr1)。系统发生组学和系统发生学分析都支持目前公认的 15 个 Paecilomyces 物种,并证实了新物种的新颖性,我们将其描述为 P. lignorum。后者是 P. brunneolus 的姊妹种,属于一个包含 P. variotii 和 P. paravariotii 的支系。从形态上看,与近缘种相比,新种能产生更长的椭圆形分生孢子,在 30 °C 的麦芽提取物琼脂上生长更受限制。引用:Visagie CM, Cruywagen EM, Duong TA (2024).一种产自南非木质电线杆的新 Paecilomyces。真菌系统学与进化 13: 163-181. doi: 10.3114/fuse.2024.13.10.
A new Paecilomyces from wooden utility poles in South Africa.
During a survey of fungi on electricity utility poles in South Africa, a diverse range of fungi were discovered. Paecilomyces was frequently isolated, with five species identified using ITS and β-tubulin (BenA) sequences. These were P. brunneolus, P. dactylethromorphus, P. lecythidis, P. paravariotii and a potential new species. The genomes of 30 of these strains were subsequently sequenced and used in a phylogenomic analysis with 45 previously published genomes of the genus. Phylogenetic analyses were also conducted using ITS, BenA, calmodulin (CaM), RNA polymerase II second largest subunit (RPB2), RNA polymerase II largest subunit (RPB1), the genes coding for the theta subunit of the TCP-1 chaperonin complex (Cct8), and for a putative ribosome biogenesis protein (Tsr1). Both phylogenomic and phylogenetic analyses supported the 15 Paecilomyces species currently accepted and confirmed the novelty of the new species, which we describe as P. lignorum. The latter is the sister species of P. brunneolus and belongs to a clade also containing P. variotii and P. paravariotii. Morphologically, the new species produces longer ellipsoidal conidia and grows more restricted on malt extract agar at 30 °C compared to its closest relatives. Citation: Visagie CM, Cruywagen EM, Duong TA (2024). A new Paecilomyces from wooden utility poles in South Africa. Fungal Systematics and Evolution13: 163-181. doi: 10.3114/fuse.2024.13.10.