{"title":"不同浓度的纳米银颗粒与氢氧化钙结合对粪肠球菌生物膜的抗生物膜效应：一项体内外研究。","authors":"Sumaya Basudan, Alanoud Alqahtani, Fatemah Alrwais, Reem Almeaither, Sayed Auda, Hanan A Balto","doi":"10.1111/aej.12882","DOIUrl":null,"url":null,"abstract":"<p><p>This study aimed to evaluate the antibiofilm activity of different concentrations of silver nanoparticles (AgNPs) in combination with calcium hydroxide [Ca(OH)<sub>2</sub>] against Enterococcus faecalis biofilm. On an E. faecalis biofilm on dentin discs, the following medicaments were applied for 7 days (n = 13/group): 0.005% AgNPs+Ca(OH)<sub>2</sub>, 0.01% AgNPs+Ca(OH)<sub>2</sub>, 0.02% AgNPs + Ca(OH)<sub>2</sub>, Ca(OH)<sub>2</sub> and saline/control. Specimens were stained with LIVE/DEAD® BacLight™ dye and analysed with confocal laser scanning microscopy. Proportion of dead bacteria was calculated and analysed. There was a significant reduction in E. faecalis biofilm in all medicament groups (43.5%, 49.1%, 69.1%, 48.7%) respectively, compared with control group (2.54%) (p < 0.001). The 0.02% AgNPs + Ca(OH)<sub>2</sub> group demonstrated the most significantly superior antibiofilm effect, with no significant difference between remaining groups. In conclusion, combining 0.02% AgNPs enhanced the antibiofilm effect of Ca(OH)<sub>2</sub> on E. faecalis biofilm compared with lower AgNPs concentrations.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antibiofilm effect of different concentrations of silver nanoparticles combined with calcium hydroxide against Enterococcus faecalis biofilm: An ex vivo study.\",\"authors\":\"Sumaya Basudan, Alanoud Alqahtani, Fatemah Alrwais, Reem Almeaither, Sayed Auda, Hanan A Balto\",\"doi\":\"10.1111/aej.12882\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study aimed to evaluate the antibiofilm activity of different concentrations of silver nanoparticles (AgNPs) in combination with calcium hydroxide [Ca(OH)<sub>2</sub>] against Enterococcus faecalis biofilm. On an E. faecalis biofilm on dentin discs, the following medicaments were applied for 7 days (n = 13/group): 0.005% AgNPs+Ca(OH)<sub>2</sub>, 0.01% AgNPs+Ca(OH)<sub>2</sub>, 0.02% AgNPs + Ca(OH)<sub>2</sub>, Ca(OH)<sub>2</sub> and saline/control. Specimens were stained with LIVE/DEAD® BacLight™ dye and analysed with confocal laser scanning microscopy. Proportion of dead bacteria was calculated and analysed. There was a significant reduction in E. faecalis biofilm in all medicament groups (43.5%, 49.1%, 69.1%, 48.7%) respectively, compared with control group (2.54%) (p < 0.001). The 0.02% AgNPs + Ca(OH)<sub>2</sub> group demonstrated the most significantly superior antibiofilm effect, with no significant difference between remaining groups. In conclusion, combining 0.02% AgNPs enhanced the antibiofilm effect of Ca(OH)<sub>2</sub> on E. faecalis biofilm compared with lower AgNPs concentrations.</p>\",\"PeriodicalId\":55581,\"journal\":{\"name\":\"Australian Endodontic Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2024-08-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Australian Endodontic Journal\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1111/aej.12882\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Australian Endodontic Journal","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1111/aej.12882","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Antibiofilm effect of different concentrations of silver nanoparticles combined with calcium hydroxide against Enterococcus faecalis biofilm: An ex vivo study.
This study aimed to evaluate the antibiofilm activity of different concentrations of silver nanoparticles (AgNPs) in combination with calcium hydroxide [Ca(OH)2] against Enterococcus faecalis biofilm. On an E. faecalis biofilm on dentin discs, the following medicaments were applied for 7 days (n = 13/group): 0.005% AgNPs+Ca(OH)2, 0.01% AgNPs+Ca(OH)2, 0.02% AgNPs + Ca(OH)2, Ca(OH)2 and saline/control. Specimens were stained with LIVE/DEAD® BacLight™ dye and analysed with confocal laser scanning microscopy. Proportion of dead bacteria was calculated and analysed. There was a significant reduction in E. faecalis biofilm in all medicament groups (43.5%, 49.1%, 69.1%, 48.7%) respectively, compared with control group (2.54%) (p < 0.001). The 0.02% AgNPs + Ca(OH)2 group demonstrated the most significantly superior antibiofilm effect, with no significant difference between remaining groups. In conclusion, combining 0.02% AgNPs enhanced the antibiofilm effect of Ca(OH)2 on E. faecalis biofilm compared with lower AgNPs concentrations.
期刊介绍：
The Australian Endodontic Journal provides a forum for communication in the different fields that encompass endodontics for all specialists and dentists with an interest in the morphology, physiology, and pathology of the human tooth, in particular the dental pulp, root and peri-radicular tissues.
The Journal features regular clinical updates, research reports and case reports from authors worldwide, and also publishes meeting abstracts, society news and historical endodontic glimpses.
The Australian Endodontic Journal is a publication for dentists in general and specialist practice devoted solely to endodontics. It aims to promote communication in the different fields that encompass endodontics for those dentists who have a special interest in endodontics.
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