动态三维细胞培养模型:应用于周围神经系统的组织工程的未来。

IF 6.7 1区 工程技术 Q1 CELL & TISSUE ENGINEERING
Journal of Tissue Engineering Pub Date : 2024-08-13 eCollection Date: 2024-01-01 DOI:10.1177/20417314241265916
William Choinière, Ève Petit, Vincent Monfette, Samuel Pelletier, Catherine Godbout-Lavoie, Marc-Antoine Lauzon
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引用次数: 0

摘要

外周神经系统(PNI)的外伤可导致瘫痪等严重后果。遗憾的是,目前的治疗方法很少能使功能得到令人满意的恢复。与外周神经系统损伤、工人残疾和患者满意度低相关的医疗费用高昂,促使人们寻求超越现有标准的替代解决方案。在治疗桥接缺损小于 30 毫米的损伤时,合成神经导管(NGC)的使用受到青睐。然而,要开发出这种前景广阔的治疗策略,还需要更忠实地模拟神经生理学的体外模型。由于缺乏具有三维环境和动态共培养等基本要素的临床比例模型,阻碍了这一领域的进展。本文的研究重点是开发一种外周神经系统(PNS)体外共培养模型,其中涉及使用微观结构复制神经地形的功能性生物材料。首先,研究了神经元衍生细胞系(N)和许旺细胞(SC)与一小段生物材料(5 毫米)接触的行为。随后使用荧光标记和存活检测进行的研究表明了共培养的协同效应。然后将这些优化参数应用于较长的生物材料(30 毫米),相当于临床使用的 NGC。结果表明,在临床规模的生物材料上维持 SC 和 N 长达 7 天的共培养是可能的,并能观察到一些功能。从长远来看,从这项工作中获得的知识将有助于更好地了解 PNS 再生过程,促进未来治疗方法的开发,同时减少对动物实验的依赖。该模型可用于药物筛选,也可用于个性化医学试验。最终,这项工作填补了目前研究中的一个重要空白,为研究和推进 PNS 损伤的治疗提供了一种变革性方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dynamic three-dimensional coculture model: The future of tissue engineering applied to the peripheral nervous system.

Traumatic injuries to the peripheral nervous system (PNI) can lead to severe consequences such as paralysis. Unfortunately, current treatments rarely allow for satisfactory functional recovery. The high healthcare costs associated with PNS injuries, worker disability, and low patient satisfaction press for alternative solutions that surpass current standards. For the treatment of injuries with a deficit of less than 30 mm to bridge, the use of synthetic nerve conduits (NGC) is favored. However, to develop such promising therapeutic strategies, in vitro models that more faithfully mimic nerve physiology are needed. The absence of a clinically scaled model with essential elements such as a three-dimension environment and dynamic coculture has hindered progress in this field. The presented research focuses on the development of an in vitro coculture model of the peripheral nervous system (PNS) involving the use of functional biomaterial which microstructure replicates nerve topography. Initially, the behavior of neuron-derived cell lines (N) and Schwann cells (SC) in contact with a short section of biomaterial (5 mm) was studied. Subsequent investigations, using fluorescent markers and survival assays, demonstrated the synergistic effects of coculture. These optimized parameters were then applied to longer biomaterials (30 mm), equivalent to clinically used NGC. The results obtained demonstrated the possibility of maintaining an extended coculture of SC and N over a 7-day period on a clinically scaled biomaterial, observing some functionality. In the long term, the knowledge gained from this work will contribute to a better understanding of the PNS regeneration process and promote the development of future therapeutic approaches while reducing reliance on animal experimentation. This model can be used for drug screening and adapted for personalized medicine trials. Ultimately, this work fills a critical gap in current research, providing a transformative approach to study and advance treatments for PNS injuries.

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来源期刊
Journal of Tissue Engineering
Journal of Tissue Engineering Engineering-Biomedical Engineering
CiteScore
11.60
自引率
4.90%
发文量
52
审稿时长
12 weeks
期刊介绍: The Journal of Tissue Engineering (JTE) is a peer-reviewed, open-access journal dedicated to scientific research in the field of tissue engineering and its clinical applications. Our journal encompasses a wide range of interests, from the fundamental aspects of stem cells and progenitor cells, including their expansion to viable numbers, to an in-depth understanding of their differentiation processes. Join us in exploring the latest advancements in tissue engineering and its clinical translation.
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