类风湿关节炎患者对甲氨蝶呤治疗差异反应的 T 细胞受体汇聚特征

IF 2.9 4区医学 Q3 IMMUNOLOGY

Immunological Investigations Pub Date : 2024-10-01 Epub Date: 2024-08-14 DOI:10.1080/08820139.2024.2381078

Taowa Zhao, Qian Zhang, Qinwen Wen, Shuyin Liu, Zitong Niu, Yang Qu, Yiting Wang, Qiaojiao Ding, Pengyao Wei, Lin Li, Tong Kong, Pan Fu, Sihua Qian, Kaizhe Wang, Xiudi Wu, Jianping Zheng

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引用次数: 0

摘要

背景：甲氨蝶呤（MTX）是治疗类风湿性关节炎（RA）的首选药物。然而，估计有 30% 至 50% 的 RA 患者对 MTX 无积极反应。虽然T细胞受体（TCR）对类风湿性关节炎的免疫反应至关重要，但它在区分MTX反应性中的作用尚未得到深入研究：本研究采用新一代测序技术分析了MTX治疗前RA患者外周血单核细胞中TCRβ链互补决定区序列。该研究旨在比较MTX应答组和非应答组的TCR序列特征：结果：研究发现，TRBV6-6 基因在 MTX 治疗反应方面存在显著差异（p = .003）。此外，在 VDJ 重排中，5'Jdels 位点的 "3 "核苷酸缺失数量也存在显著差异（p = .023）：这些研究结果表明，在RA患者中，MTX应答组和非应答组的TCR基因组具有不同的特征。这一发现为了解 RA 患者对 MTX 治疗的不同反应提供了新的视角。

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Characteristics of T-Cell Receptor Repertoire for Differential Response to Methotrexate Treatment for Rheumatoid Arthritis.

Background: Methotrexate (MTX) serves as the initial treatment for rheumatoid arthritis (RA). However, a substantial proportion of RA patients, estimated between 30% and 50%, do not respond positively to MTX. While the T-cell receptor (TCR) is crucial for the immune response during RA, its role in differentiating MTX responsiveness has not been thoroughly investigated.

Methods: This study used next-generation sequencing to analyze the TCR β-chain complementary determining region sequences in peripheral blood mononuclear cells obtained from RA patients before MTX treatment. This study aimed to compare the characteristics of the TCR repertoire between the MTX responder and non-responder groups.

Results: The study identified a significant difference in the TRBV6-6 gene (p = .003) concerning MTX treatment response. Additionally, a significant difference was found in the number of "3" nucleotide deletions at the 5'Jdels site (p = .023) in the VDJ rearrangement.

Conclusion: These findings suggest distinct TCR repertoire characteristics between MTX responder and non-responder groups among RA patients. This discovery offers new insights into understanding the variable responses of RA patients to MTX therapy.

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来源期刊

Immunological Investigations 医学-免疫学

CiteScore

5.50

自引率

7.10%

发文量

审稿时长

3 months

期刊介绍： Disseminating immunological developments on a worldwide basis, Immunological Investigations encompasses all facets of fundamental and applied immunology, including immunohematology and the study of allergies. This journal provides information presented in the form of original research articles and book reviews, giving a truly in-depth examination of the latest advances in molecular and cellular immunology.