探索细胞周期相关基因 CHMP4C 在前列腺癌中的临床和生物学意义。

IF 2.1 4区 医学 Q3 GENETICS & HEREDITY
Xi Xiao, Zonglin Li, Qingchao Li, Liangliang Qing, Yanan Wang, Fuxiang Ye, Yajia Dong, Xiaoyu Di, Jun Mi
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引用次数: 0

摘要

背景:前列腺癌(PCa)是影响男性健康的第二大高发恶性肿瘤,该疾病的演变过程给患者的治疗和预后管理带来了严峻的挑战。带电多囊体蛋白4 C(CHMP4C)通过调节细胞周期功能参与多种癌症的发展。然而,CHMP4C 在前列腺癌中的作用仍不清楚:在生物信息学方面,我们利用多个 PCa 数据集仔细研究了 CHMP4C 的表达。方法:在生物信息学方面,研究人员利用多个 PCa 数据集仔细研究了 CHMP4C 的表达,并采用生存分析和提名图法来探究 CHMP4C 的预后意义。基因组富集分析(Gene set enrichment analysis,GSEA)用于探究CHMP4C的功能意义。在细胞实验方面,利用 qRT-PCR 和 Western 印迹技术验证了 RNA 和蛋白质的表达水平。在建立了稳定敲除 CHMP4C 的细胞系后,我们采用了一系列检测方法,包括细胞计数试剂盒-8(CCK-8)、伤口愈合、Transwell 和流式细胞术,以确定 CHMP4C 对 PCa 细胞的增殖、迁移、侵袭和细胞周期功能的影响:结果:CHMP4C在PCa细胞和组织中的表达均呈上调趋势,CHMP4C表达水平升高的患者预后明显较差。利用 CHMP4C 和临床病理特征构建的提名图显示出了值得称道的预后预测能力。敲除 CHMP4C 能显著抑制 PCa 细胞(LNcaP 和 PC3)的增殖、迁移和侵袭。CHMP4C可影响PCa细胞周期的进展,其表达可能受小檗碱调控。PCa患者之间不同的CHMP4C表达可能会引起免疫细胞浸润和基因突变频率的改变:我们的研究结果表明,CHMP4C可能是PCa的预后生物标志物,为推进PCa的精准治疗提供了新的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Exploring the clinical and biological significance of the cell cycle-related gene CHMP4C in prostate cancer.

Background: Prostate cancer (PCa) stands as the second most prevalent malignancy impacting male health, and the disease's evolutionary course presents formidable challenges in the context of patient treatment and prognostic management. Charged multivesicular body protein 4 C (CHMP4C) participates in the development of several cancers by regulating cell cycle functions. However, the role of CHMP4C in prostate cancer remains unclear.

Methods: In terms of bioinformatics, multiple PCa datasets were employed to scrutinize the expression of CHMP4C. Survival analysis coupled with a nomogram approach was employed to probe into the prognostic significance of CHMP4C. Gene set enrichment analysis (GSEA) was conducted to interrogate the functional implications of CHMP4C. In terms of cellular experimentation, the verification of RNA and protein expression levels was executed through the utilization of qRT-PCR and Western blotting. Upon the establishment of a cell line featuring stable CHMP4C knockdown, a battery of assays, including Cell Counting Kit-8 (CCK-8), wound healing, Transwell, and flow cytometry, were employed to discern the impact of CHMP4C on the proliferation, migration, invasion, and cell cycle function of PCa cells.

Results: The expression of CHMP4C exhibited upregulation in both PCa cells and tissues, and patients demonstrating elevated CHMP4C expression levels experienced a notably inferior prognosis. The nomogram, constructed using CHMP4C along with clinicopathological features, demonstrated a commendable capacity for prognostic prediction. CHMP4C knockdown significantly inhibited the proliferation, migration, and invasion of PCa cells (LNcaP and PC3). CHMP4C could impact the advancement of the PCa cell cycle, and its expression might be regulated by berberine. Divergent CHMP4C expression among PCa patients could induce alterations in immune cell infiltration and gene mutation frequency.

Conclusions: Our findings suggest that CHMP4C might be a prognostic biomarker in PCa, potentially offering novel perspectives for the advancement of precision therapy for PCa.

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来源期刊
BMC Medical Genomics
BMC Medical Genomics 医学-遗传学
CiteScore
3.90
自引率
0.00%
发文量
243
审稿时长
3.5 months
期刊介绍: BMC Medical Genomics is an open access journal publishing original peer-reviewed research articles in all aspects of functional genomics, genome structure, genome-scale population genetics, epigenomics, proteomics, systems analysis, and pharmacogenomics in relation to human health and disease.
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