揭示自交系鲫鱼的潜在性别决定基因和性别特异性标记。

IF 8 2区 生物学 Q1 BIOLOGY
Science China Life Sciences Pub Date : 2024-11-01 Epub Date: 2024-08-09 DOI:10.1007/s11427-023-2694-5
Kun Zhang, Xu Huang, Chongqing Wang, Xidan Xu, Xiaowei Xu, Xiaoping Dong, Qingwen Xiao, Jinhai Bai, Yue Zhou, Zhengkun Liu, Xinyi Deng, Yan Tang, Siyang Li, Enkui Hu, Wanjing Peng, Ling Xiong, Qinbo Qin, Shaojun Liu
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引用次数: 0

摘要

自四倍体鲫鱼(Carassius auratus)是一种稳定的遗传性自四倍体鱼类,由鲫鱼红变种(Carassius auratus red var., RCC, ♀)×大鳞鲫(Megalobrama amblycephala, BSB, ♂)杂交而成,含有四组RCC染色体。然而,该物种性别决定的分子机制在很大程度上仍然未知。目前,人们对该物种性别决定的分子机制和区分性别的特异性分子标记缺乏全面了解。本研究通过对 100 个个体(包括 50 个雌性个体和 50 个雄性个体)进行全基因组重测序,获得了 25,801,677 个 SNPs(单核苷酸多态性)和 6,210,306 个 Indels(插入缺失)。进一步鉴定确认候选染色体为 Chr46B,性别决定区位于 Chr46B:22,500,000-22,800,000 bp。根据候选性别决定区内的雄性特异性插入(26 bp),确定了一对性别特异性分子标记。此外,根据对候选性别决定区基因的筛选和 RT-qPCR 验证分析,ADAM10、AQP9 和 tc1a 被确定为候选性别决定基因。这些发现为研究鱼类的性别决定机制、性染色体的进化以及单性种群的发展奠定了坚实的基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Unveiling potential sex-determining genes and sex-specific markers in autotetraploid Carassius auratus.

Autotetraploid Carassius auratus is a stable hereditary autotetraploid fish resulting from the hybridization of Carassius auratus red var. (RCC, ♀) × Megalobrama amblycephala (BSB, ♂), containing four sets of RCC chromosomes. However, the molecular mechanism underlying the determination of sex in this species remains largely unknown. Currently, there lacks a full understanding of the molecular mechanisms governing sex determination and specific molecular markers to differentiate sex in this species. In this study, 25,801,677 SNPs (Single-nucleotide polymorphism) and 6,210,306 Indels (insertion-deletion) were obtained from whole-genome resequencing of 100 individuals (including 50 female and 50 male). Further identification confirmed the candidate chromosomes as Chr46B, with the sex-determining region located at Chr46B: 22,500,000-22,800,000 bp. Based on the male-specific insertion (26 bp) within the candidate sex-determining region, a pair of sex-specific molecular markers has been identified. In addition, based on the screening of candidate sex-determining region genes and RT-qPCR validation analysis, ADAM10, AQP9 and tc1a were identified as candidate sex-determining genes. These findings provide a robust foundation for investigating sex determination mechanisms in fish, the evolution of sex chromosomes, and the development of monosex populations.

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来源期刊
CiteScore
15.10
自引率
8.80%
发文量
2907
审稿时长
3.2 months
期刊介绍: Science China Life Sciences is a scholarly journal co-sponsored by the Chinese Academy of Sciences and the National Natural Science Foundation of China, and it is published by Science China Press. The journal is dedicated to publishing high-quality, original research findings in both basic and applied life science research.
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