线粒体源性区室是一个多纤层结构域,可容纳膜货物和细胞膜。

IF 7.4 1区 生物学 Q1 CELL BIOLOGY
Journal of Cell Biology Pub Date : 2024-11-04 Epub Date: 2024-08-13 DOI:10.1083/jcb.202307035
Zachary N Wilson, Matt West, Alyssa M English, Greg Odorizzi, Adam L Hughes
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引用次数: 0

摘要

保持线粒体网络的健康对细胞的活力和寿命至关重要。为此,线粒体采用了多种膜重塑机制,包括形成线粒体衍生囊泡 (MDV) 和区室 (MDC),以选择性地移除细胞器的一部分。与表征明确的 MDVs 不同,MDCs 形成和组成的显著特征仍不清楚。在这里,我们利用电子断层扫描技术观察到,MDCs 以大型多纤毛结构域的形式形成,在 ER 线粒体与线粒体的接触部位产生从线粒体小管出现的同心球形隔室。对 MDC 生物发生过程的延时荧光显微镜观察发现,线粒体膜延伸部分反复伸长、凝聚和内陷,形成了这些包裹多层膜的区室。因此,MDCs 能强力封闭线粒体外膜的一部分,将膜货物固定在一个受保护的区域内,同时也将细胞质封闭在 MDC 内腔中。总之,我们的研究结果为 MDC 的形成提供了一个模型,并描述了将 MDC 与之前发现的其他线粒体结构和货物分类域区分开来的关键特征。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mitochondrial-derived compartments are multilamellar domains that encase membrane cargo and cytosol.

Preserving the health of the mitochondrial network is critical to cell viability and longevity. To do so, mitochondria employ several membrane remodeling mechanisms, including the formation of mitochondrial-derived vesicles (MDVs) and compartments (MDCs) to selectively remove portions of the organelle. In contrast to well-characterized MDVs, the distinguishing features of MDC formation and composition remain unclear. Here, we used electron tomography to observe that MDCs form as large, multilamellar domains that generate concentric spherical compartments emerging from mitochondrial tubules at ER-mitochondria contact sites. Time-lapse fluorescence microscopy of MDC biogenesis revealed that mitochondrial membrane extensions repeatedly elongate, coalesce, and invaginate to form these compartments that encase multiple layers of membrane. As such, MDCs strongly sequester portions of the outer mitochondrial membrane, securing membrane cargo into a protected domain, while also enclosing cytosolic material within the MDC lumen. Collectively, our results provide a model for MDC formation and describe key features that distinguish MDCs from other previously identified mitochondrial structures and cargo-sorting domains.

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来源期刊
Journal of Cell Biology
Journal of Cell Biology 生物-细胞生物学
CiteScore
12.60
自引率
2.60%
发文量
213
审稿时长
1 months
期刊介绍: The Journal of Cell Biology (JCB) is a comprehensive journal dedicated to publishing original discoveries across all realms of cell biology. We invite papers presenting novel cellular or molecular advancements in various domains of basic cell biology, along with applied cell biology research in diverse systems such as immunology, neurobiology, metabolism, virology, developmental biology, and plant biology. We enthusiastically welcome submissions showcasing significant findings of interest to cell biologists, irrespective of the experimental approach.
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