游离液层数量对细菌和蛋白质粘附在注入液体的聚合物上的影响。

IF 1.6 4区 医学 Q4 BIOPHYSICS
Biointerphases Pub Date : 2024-07-01 DOI:10.1116/6.0003776
ChunKi Fong, Marissa Jeme Andersen, Emma Kunesh, Evan Leonard, Donovan Durand, Rachel Coombs, Ana Lidia Flores-Mireles, Caitlin Howell
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引用次数: 0

摘要

注入液体的聚合物具有排斥污物的能力,因此在生物医学(包括导管相关性尿路感染(CAUTIs))领域具有广阔的应用前景。然而,人们对表面覆盖的游离液体层的数量对蛋白质和细菌粘附性的影响还不甚了解。在此,我们探讨了灌注硅胶导管材料中游离硅胶液体层的数量如何影响与 CAUTI 相关的细菌和蛋白质的粘附。为了改变游离液体层的数量,我们用物理方法去除完全灌注导管材料中多余的液体,或者部分灌注导管材料。然后我们评估了其对细菌和宿主蛋白质粘附的影响。与对照组相比,从完全灌注的样品中物理去除游离液体层后,液体层的高度从 60 μm 降至检测限以下,硅胶液体流失到环境中的量减少了约 64%,但蛋白质纤维蛋白原的沉积或常见尿路病原体粪肠球菌的粘附却没有明显增加。部分输液的样本显示出更大的液体流失减少量:与完全输液的对照组相比,输液量达到最大容量 70%-80% 的样本显示出约 85% 的液体流失减少量。值得注意的是,输液量超过 70% 的样本中纤维蛋白原或粪肠球菌粘附量没有明显增加。这些研究结果表明,调整灌注聚合物中游离液体层的水平可影响其表面的蛋白质和细菌粘附性。此外,去除游离液层可有效减少这些聚合物的液体流失,同时保持其功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of free liquid layer quantity on bacteria and protein adhesion to liquid infused polymers.

Liquid-infused polymers are recognized for their ability to repel foulants, making them promising for biomedical applications including catheter-associated urinary tract infections (CAUTIs). However, the impact of the quantity of free liquid layer covering the surface on protein and bacterial adhesion is not well understood. Here, we explore how the amount of free silicone liquid layer in infused silicone catheter materials influences the adhesion of bacteria and proteins relevant to CAUTIs. To alter the quantity of the free liquid layer, we either physically removed excess liquid from fully infused catheter materials or partially infused them. We then evaluated the impact on bacterial and host protein adhesion. Physical removal of the free liquid layer from the fully infused samples reduced the height of the liquid layer from 60 μm to below detection limits and silicone liquid loss into the environment by approximately 64% compared to controls, without significantly increasing the deposition of protein fibrinogen or the adhesion of the common uropathogen Enterococcus faecalis. Partially infused samples showed even greater reductions in liquid loss: samples infused to 70%-80% of their maximum capacity exhibited about an 85% decrease in liquid loss compared to fully infused controls. Notably, samples with more than 70% infusion did not show significant increases in fibrinogen or E. faecalis adhesion. These findings suggest that adjusting the levels of the free liquid layer in infused polymers can influence protein and bacterial adhesion on their surfaces. Moreover, removing the free liquid layer can effectively reduce liquid loss from these polymers while maintaining their functionality.

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来源期刊
Biointerphases
Biointerphases 生物-材料科学:生物材料
自引率
0.00%
发文量
35
期刊介绍: Biointerphases emphasizes quantitative characterization of biomaterials and biological interfaces. As an interdisciplinary journal, a strong foundation of chemistry, physics, biology, engineering, theory, and/or modelling is incorporated into originated articles, reviews, and opinionated essays. In addition to regular submissions, the journal regularly features In Focus sections, targeted on specific topics and edited by experts in the field. Biointerphases is an international journal with excellence in scientific peer-review. Biointerphases is indexed in PubMed and the Science Citation Index (Clarivate Analytics). Accepted papers appear online immediately after proof processing and are uploaded to key citation sources daily. The journal is based on a mixed subscription and open-access model: Typically, authors can publish without any page charges but if the authors wish to publish open access, they can do so for a modest fee. Topics include: bio-surface modification nano-bio interface protein-surface interactions cell-surface interactions in vivo and in vitro systems biofilms / biofouling biosensors / biodiagnostics bio on a chip coatings interface spectroscopy biotribology / biorheology molecular recognition ambient diagnostic methods interface modelling adhesion phenomena.
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