用豆类蛋白分离物稳定的浓缩水包油型乳液中蛋白质和脂质氧化的时空评估

IF 6.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY
Mariska Brüls-Gill , Vincent J.P. Boerkamp , Johannes Hohlbein , John P.M. van Duynhoven
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引用次数: 0

摘要

用植物蛋白替代动物蛋白的趋势日益明显,这就要求人们更多地了解素食蛋黄酱的功能性和稳定性,尤其是它们对脂质和蛋白质氧化的敏感性。在这里,我们研究了用豆类((水解)大豆、豌豆和蚕豆)蛋白分离物(hSPI、SPI、PPI、FPI)稳定的乳液中脂质和蛋白质氧化的空间和时间动态。我们通过核磁共振和激光共聚焦扫描显微镜,使用对氧化敏感的荧光染料 BODIPY 665/676 评估了脂质的整体氧化情况和局部氧化情况。此外,我们还利用蛋白质自发荧光和荧光标记的自由基自旋捕获剂 CAMPO-AFDye 647 评估了局部蛋白质氧化情况。油滴中油的氧化受油相中存在的生育酚和通过蛋白质分离物引入的促氧化过渡金属的影响。与使用 SPI 和 FPI 制备的乳液相比,使用 PPI 和 hSPI 稳定的非剥离油乳液显示出更高水平的脂质氢过氧化物。我们将这一发现归因于 PPI 和 hSPI 中催化活性过渡金属的可用性更高。对于用 SPI 和 FPI 稳定的剥离油乳剂,在抗坏血酸存在的情况下,脂质氢过氧化物的浓度可以忽略不计,这表明抗坏血酸起到了抗氧化剂的作用。对于用 PPI 和 hSPI 制备的乳液,抗坏血酸只能部分抑制脂质过氧化氢的形成,这表明抗坏血酸起到了促氧化剂的作用。有趣的是,我们在所有乳液中都观察到了蛋白质-脂质聚集体。这些聚集体发生了快速而广泛的共氧化作用,这种作用也受到过渡金属和来自油相的生育酚的调节。我们的研究证明了时空成像技术在增进我们对植物蛋白稳定乳液氧化过程的了解方面所具有的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Spatiotemporal assessment of protein and lipid oxidation in concentrated oil-in-water emulsions stabilized with legume protein isolates

Spatiotemporal assessment of protein and lipid oxidation in concentrated oil-in-water emulsions stabilized with legume protein isolates

The growing trend of substituting animal-based proteins with plant-based proteins requires more understanding of the functionality and stability of vegan mayonnaises, especially regarding their susceptibility to lipid and protein oxidation. Here, we investigate the spatial and temporal dynamics of lipid and protein oxidation in emulsions stabilized with legume ((hydrolyzed) soy, pea, and faba bean) protein isolates (hSPI, SPI, PPI, FPI). We assessed lipid oxidation globally by NMR and locally by confocal laser scanning microscopy using the oxidation-sensitive fluorescent dye BODIPY 665/676. Further, we assessed local protein oxidation by employing protein autofluorescence and the fluorescently labeled radical spin-trap CAMPO-AFDye 647. Oxidation of oil in droplets was governed by the presence of tocopherols in the oil phase and pro-oxidant transition metals that were introduced via the protein isolates. Non-stripped oil emulsions stabilized with PPI and hSPI displayed higher levels of lipid hydroperoxides as compared to emulsions prepared with SPI and FPI. We attribute this finding to higher availability of catalytically active transition metals in PPI and hSPI. For stripped oil emulsions stabilized with SPI and FPI, lipid hydroperoxide concentrations were negligible in the presence of ascorbic acid, indicating that this agent acted as antioxidant. For the emulsions prepared with PPI and hSPI, lipid hydroperoxide formation was only partly inhibited by ascorbic acid, indicating a role as prooxidant. Interestingly, we observed protein-lipid aggregates in all emulsions. The aggregates underwent fast and extensive co-oxidation, which was also modulated by transition metals and tocopherols originating from the oil phase. Our study demonstrates the potential of spatiotemporal imaging techniques to enhance our understanding of the oxidation processes in emulsions stabilized with plant proteins.

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来源期刊
Current Research in Food Science
Current Research in Food Science Agricultural and Biological Sciences-Food Science
CiteScore
7.40
自引率
3.20%
发文量
232
审稿时长
84 days
期刊介绍: Current Research in Food Science is an international peer-reviewed journal dedicated to advancing the breadth of knowledge in the field of food science. It serves as a platform for publishing original research articles and short communications that encompass a wide array of topics, including food chemistry, physics, microbiology, nutrition, nutraceuticals, process and package engineering, materials science, food sustainability, and food security. By covering these diverse areas, the journal aims to provide a comprehensive source of the latest scientific findings and technological advancements that are shaping the future of the food industry. The journal's scope is designed to address the multidisciplinary nature of food science, reflecting its commitment to promoting innovation and ensuring the safety and quality of the food supply.
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