用于大视场神经活动双色成像的轻型微型内窥镜

Q3 Physics and Astronomy

Results in Optics Pub Date : 2024-07-01 DOI:10.1016/j.rio.2024.100732

Loïc Tabourin , Frédéric Bretzner , Tigran Galstian

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引用次数: 0

摘要

我们介绍了一种微型 "便携式 "迷你内窥镜的设计和实验特性，这种内窥镜具有大视野（≈360rmmum），可使用两个光谱波段，分别适用于 mCherry（峰值发射波长为 610 nm）和 GCaMP6s（峰值发射波长为 513 nm）荧光团。植入物的直径非常小（0.5 毫米），总重量为 1.8 克，大小为 10×12×21mm3，装置的横向分辨率≈4.5μm。这种小巧轻便的系统可用于研究两种不同神经元群的神经活动。

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Lightweight mini-endoscope for two color imaging of neural activities with large field of view

We present the design and the experimental characterization of a miniature “portable” mini-endoscope with a large field-of-view ( $\approx 360 r m m u m$ ) that operates with two spectral bands adapted for mCherry (peak emission at 610 nm) and GCaMP6s (peak emission at 513 nm) fluorophores. The diameter of the implant is very small (0.5 mm), the total weight is 1.8 g, the size is $10 \times 12 \times 21 {mm}^{3}$ , and the lateral resolution of the device is $\approx 4.5 μ m$ . This compact and lightweight system allows investigation of the neural activity of two different neuronal populations.