人类 IgG 亚类能否区分确诊和未确诊的 SARS-CoV-2 感染?

IF 3.5 Q3 Biochemistry, Genetics and Molecular Biology
Mahmoud Mohamed Bahgat , Mohamed Hassan Nasraa , Rola Nadeem , Khaled Amer , Wael A. Hassan , Ahmed Abd EL-Raouf , Dina Nadeem Abd-Elshafy
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引用次数: 0

摘要

背景免疫球蛋白 G (IgG) 亚类在病毒感染的免疫反应中起着至关重要的作用。虽然总 IgG 水平通常能说明免疫反应的情况,但特定的 IgG 亚类能提供有关免疫反应性质和感染阶段的更详细信息。在此,我们探讨了总 IgG 亚类(t)和 SARS-CoV-2 特异性 IgG 亚类(s)在区分感染确诊病毒-qRT-PCR 阳性(IC;V-qRT-PCR-P)和感染未确诊病毒-qRT-PCR 未检查(IU;V-qRT-PCR-UC)埃及人方面的价值。结果 在 IU 受试者中,t-IgG2 和 4 的平均值均明显较高(SH),而在 IC 受试者中,s-IgG1 和 3 的平均值均为 SH。在性别方面,IU 女性的 tgG2 和 4 平均值均为 SH,而 IC 女性的 s-IgG1 平均值为 SH。IU 男性的 t-IgG4 平均值为 SH,而 IC 男性的 s-IgG1 和 3 平均值均为 SH。在 IU 人类中,t-IgG1 和 3 与症状分级(SG)之间存在显著的正相关(SPC)(r2 分别为 0.200 和 0.253)。此外,在 IU 女性中,s-IgG2 与 SG 之间也存在 SPC(r2 = 0.6782)。在 IU 男性中,t-IgG1 和 s-IgG2 与 SG 之间存在 SPC(r2 分别为 0.794 和 0.373)。结论尽管研究样本量较小,但我们的研究结果表明,一些总的和SARS-CoV-2特异性IgG亚类是区分确诊和未确诊SARS-CoV-2感染的补充性和性别特异性免疫标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Can human IgG subclasses distinguish between confirmed and unconfirmed SARS-CoV-2 infections?

Background

Immunoglobulin G (IgG) subclasses play a crucial role in the immune response to viral infections. While total IgG levels can generally provide an indication on the immune response, specific IgG subclasses can offer more detailed information about nature of the immune response and stage of the infection. Herein, we addressed the value of both total (t) and SARS-CoV-2-specific (s) IgG-subclasses in distinguishing between infection-confirmed virus-qRT-PCR-positive (IC; V-qRT-PCR-P) and infection-unconfirmed virus-qRT-PCR-unchecked (IU; V-qRT-PCR-UC) Egyptians.

Results

Both the t-IgG2 and 4 means were significantly higher (SH) among the IU subjects, whereas, the s-IgG1 and 3 means were SH among the IC ones. On the gender levels, both the t-IgG2 and 4 means were SH among the IU females, whereas, the mean of the s-IgG1 was SH among the IC females. The t-IgG4 mean was SH among the IU males, whereas, both means of the s-IgG1 and 3 were SH among the IC males. Significant positive correlations (SPC) were recorded between both the t-IgG1 and 3 with the symptom grades (SG) among the IU humans (r2 = 0.200 and 0.253, respectively). Also, SPC was noticed between the s-IgG2 and the SG among the IU females (r2 = 0.6782). SPC was recorded between both the t-IgG1 and the s-IgG2 with the SG among the IU males (r2 = 0.794 and 0.373, respectively). SPC was noticed between the t-IgG3 and the age among the IC males (r2 = 0.779).

Conclusion

Although the limitation of the small studied sample size, our results suggest some total and SARS-CoV-2-specific IgG-subclasses as both supplemental and gender-specific immune markers to distinguish between confirmed and unconfirmed SARS-CoV-2 infections.

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来源期刊
Journal of Genetic Engineering and Biotechnology
Journal of Genetic Engineering and Biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
5.70
自引率
5.70%
发文量
159
审稿时长
16 weeks
期刊介绍: Journal of genetic engineering and biotechnology is devoted to rapid publication of full-length research papers that leads to significant contribution in advancing knowledge in genetic engineering and biotechnology and provide novel perspectives in this research area. JGEB includes all major themes related to genetic engineering and recombinant DNA. The area of interest of JGEB includes but not restricted to: •Plant genetics •Animal genetics •Bacterial enzymes •Agricultural Biotechnology, •Biochemistry, •Biophysics, •Bioinformatics, •Environmental Biotechnology, •Industrial Biotechnology, •Microbial biotechnology, •Medical Biotechnology, •Bioenergy, Biosafety, •Biosecurity, •Bioethics, •GMOS, •Genomic, •Proteomic JGEB accepts
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