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引用次数: 0
摘要
舒尼替尼(SUN)是通过靶向细胞膜上的受体酪氨酸激酶(RTK)治疗肾透明癌细胞的一线药物。然而,如何将舒尼替尼有效地输送到细胞膜上仍然是一个巨大的挑战。在这项研究中,我们制作了具有强表面SUN粘附性的精确结构DNA纳米带,从而实现了对肾透明癌细胞的RTK抑制。在我们的设计中,以精确组装的线性拓扑六螺旋束 DNA 折纸为框架,将带正电荷的壳聚糖吸附在 DNA 折纸表面,从而形成 DNA 纳米带。通过静电作用,SUN 被有效地载入 DNA 纳米带表面。我们发现,DNA 纳米带在血清中表现出卓越的稳定性。重要的是,携带 SUN 的 DNA 纳米带可以延长细胞膜保留时间,抑制 RTK,从而增强对 786-0 细胞的细胞毒性。综上所述,这项研究为在抗癌治疗中高效递送细胞膜受体抑制剂提供了一个前景广阔的候选平台。
DNA Origami-Constructed Nanotapes for Sunitinib Adsorption and Inhibition of Renal Clear Carcinoma Cells
Sunitinib (SUN) is a first-line drug for the treatment of renal clear carcinoma cells by targeting receptor tyrosine kinases (RTK) on the cell membrane. However, the effective delivery of SUN to the cell membrane remains a significant challenge. In this study, we fabricated precisely structured DNA nanotapes with strong surface SUN adhesion, enabling RTK inhibition of renal clear carcinoma cells. In our design, the precisely assembled linear topological six-helical-bundle DNA origami serves as the framework, and positively charged chitosan is adsorbed onto the DNA origami surface, thereby forming DNA nanotapes. The SUN was efficiently loaded onto the surface of the DNA nanotapes by electrostatic interaction. We found that DNA nanotapes exhibit excellent stability in serum. Importantly, DNA nanotapes carrying SUN can achieve prolonged cell membrane retention and inhibit RTK, thereby enhancing cytotoxicity toward 786-0 cells. Taken together, this study provides a promising candidate platform for the efficient delivery of cell membrane receptor inhibitors in anticancer therapy.
ACS OmegaChemical Engineering-General Chemical Engineering
CiteScore
6.60
自引率
4.90%
发文量
3945
审稿时长
2.4 months
期刊介绍:
ACS Omega is an open-access global publication for scientific articles that describe new findings in chemistry and interfacing areas of science, without any perceived evaluation of immediate impact.